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Methods: PNS was got by extracting panax notognseng with general method of alcohol circumfluence, purified with organic solvents, and separated with D101 macroreticular resin. The hematolysis rate and the immunocompetence of biology of PNS were determined by hemolysis test and experiment of splenocyte proliferation respectively.

乙醇回流法提取PNS粗提物，经有机溶剂除杂后，通过大孔树脂柱层析分离纯化；溶血性试验测定PNS作用后红细胞的溶血率；脾淋巴细胞增殖反应测定PNS的生物免疫活性。

In this dissertation, several recombinant unlabeled as well as 15N- or 15N/13C-labeled proteins were expressed and purified in large quantities. We then carried out circular dichroism and heteronuclear multidimensional NMR experiments to gain insight into the structure-function relationships on these proteins, as described below.

在本篇论文里，我们表现并纯化出大量所欲探讨的重组蛋白质，利用碳-13与氮-15同位素来标定这些重组蛋白，以旋光光谱及异核多维核磁共振技术来探讨这些蛋白质的结构与其功能的相关性，内容如下

Then the fusion protein was primary purified by affinity chromatography. The GST tail of the fusion protein was cleaved by Thrombin. Nucleotide sequence analysis showed that the gdh gene of S.suis possesses the highly conserved motifs typical of family I hexameric GDHs.

猪链球菌2型谷氨酸脱氢酶编码基因克隆、序列分析及原核表达根据已发表的猪链球菌2型的gdh基因设计引物，扩增海安病人分离株Habb中的目的基因，并进行序列测定和分析；构建重组表达载体pGEX4T-2-gdh，在大肠杆菌中表达，并纯化重组蛋白。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的，我们设计了以下的实验步骤﹔（1）二次代谢物回收及检测样本处理：我们将发酵所得的培养上清液，利用疏水吸附性树酯Amberlite XAD-2吸附回收，并以甲醇溶离及真空减压浓缩脱水等方式处理，以取得提供抗氧化活性筛选之检测样本；（2）抗氧化活性检测及菌种筛选：以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用；经此筛选程序，我们选获了具有抗氧化物质高生产力的菌株，命名为AMBL-029C；（3）抗氧化物质的分离纯化：针对生产菌株的发酵回收处理液，以矽胶薄层色层分离法经物质层析纯化后，并以高效能液相层析法(High performance liquid chromatography；HPLC)分析物质可得一初级纯化物质，命名为AMBL-029C-TS；（4）抗氧化物质的物理化学性质分析：由酸碱转溶（acid-base fractionation）实验得知，此抗氧化物质属於中低极性的强酸性物质，对温度（37℃-100℃）及酸碱度（pH3.0-13.0）均表现出高稳定性；（5）在抗氧化机制探讨方面，我们针对数种不同的抗氧化机制进行探讨，即： 1。

This thesis tells that we have made progress to the pharmaceutical water system in Hebei Huari pharmaceutical Co. Ltd. We integrated theory and practice, obeyed the demand of U.S.P. and C.P. We improved the unreasonable thing in original purified water and water for injection producing design.

本次论文阐述了在对河北华日公司制药用水系统的工艺设计改进过程中，遵照美国药典及我国GMP的要求，结合理论与实践，对原有纯化水及注射用水制备设计中的不合理之处进行了相应的改进。

By using these antibodies, which were raised to immunoreact with total proteins of purified mitochondria from different organs of mung bean seedlings, we find that there were two hybridizable AOX bands in mitochondria in mung bean seedlings.

将此抗体与来自绿豆幼苗不同器官线粒体的总蛋白分别进行Western杂交，可观察到绿豆幼苗线粒体中具有两条清晰的AOX杂交带，它们的分子量分别为35kD和38kD。

Based on biological experiments, mango glycoside is used as raw material; under the condition with phenol and hydroiodic acid, carbon-carbon bonds are broken to produce the crude product of mango aglycone; the crude product is separated and purified through silica gel column chromatography so that the mango aglycone is separated; the yield rate reaches 12 percent, the purity is higher than 95 percent, and the reproduction performance is good; the IGTT of C57BL/6 normal mice is effectively improved with the norathyriol; and simultaneously the quantity of insulin secretion induced by glucose in vivo for the C57BL/6 normal mice can be improved with the norathyriol.

通过生物学实验证明，本发明所涉及的以芒果苷为原料，在苯酚和氢碘酸条件下，使得碳-碳键断裂，制备得到芒果苷元粗品，再通过硅胶柱层析分离纯化得到芒果苷元的方法使得其产率可达12％、纯度大于95％，且重现性好；norathyriol可使C57BL/6正常小鼠IGTT有明显改善；同时norathyriol可提高C57BL/6正常小鼠体内葡萄糖诱导的胰岛素分泌量。

Some parameters and properties of Centrin, after being purified, were predicted with GenePro, such as isoelectric point, net charge, amphipathy, hydropathy, a-helix potential, Calcium-binding site. All these attempts aim to provide some theoretical guidance for the succeeding experiential researches.

纯化后的Centrin先运用GenePro对它的一些参数及性质做了预测，诸如等电点、净电荷量、亲水亲脂性、α-螺旋分布及钙结合区氨基酸序列分布等，以期为后续实验研究起到理论性的指导作用。

Secondly, although protein sequence hydropathy prediction result showed ZmDWF1 is an integrated membrane protein, we still successfully expressed the full-length protein fused into the plasmid pET30a , then purified the 70 KD fusion proteins by Ni-NTA agarose affinity chromatography column.

其次，尽管蛋白氨基酸序列的疏水性分析表明，ZmDWF1是一种整合膜蛋白，我们依然将ZmDWF1全长序列克隆到原核表达载体pET30a中，并成功的在大肠杆菌BL21（DE3）中表达了这种预测的膜蛋白全长。

We inoculated the hybridoma into Intraperitoneal mice and hydroperitoneum was purified with salt precipitation.

取腹水经盐析法初次提纯HCMV McAbs。

Recall that, in the Kolmogorov axiomatization, measurable sets are the sets which have a probability or, in other words, the sets corresponding to yes/no questions that have a probabilistic answer.

延期开工的Kolmogorov宣布要衡量所有套职能有限许多坐标限制在于衡量子寅。换句话说，如果是/否的问题回答f可看的价值观念在许多最有限坐标，然后它有一个概率的答案。

The experimental results showed that the algorithm can filter singular points and remain fixity of the target edge.

实验表明：基于模糊理论的图像智能滤波方法比常规图像滤波方法运算量小，它既能去除奇异点，又能保持图像中目标的边缘不变性，具有很好的滤波效果。