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In order to develop antibacterial peptide biologics for prevention and control of endometritis of dairy cow,a pair of primers was designed and synthesized according to the sequence of bovine antibacterial peptide gene published in GenBank.The total RNA was purified from the dairy cow endometrial cells with the Total RNA Isolation Kit.

为研发防治乳牛子宫内膜炎的抗菌肽生物制剂，根据已发表的牛抗菌肽基因序列设计了1对引物，用试剂盒提取乳牛新鲜子宫内膜总RNA，经RT-PCR扩增，将克隆的抗菌肽BNBD5基因全长编码区的cDNA连接到原核表达载体pET30a中，经IPTG诱导原核表达，通过Western-blot检测融合蛋白BNBD5的表达水平。

The purified PAT was identified by Western blot and enzymic activity analysis.

首先通过Western杂交和酶活性分析鉴定了PAT蛋白的纯度和活性。

It has been reported that NDP kinase has weak interaction with Hsp70, which can increase the ATP hydrolysis activity, so we carefully examined the possibility of NDP kinase contamination in the experiment. The equimolar mixture of NDP kinase and Hsp70 were digested and then 44-kD fragment was purified.

为获得准确的结论排除NDP激酶可能对实验的影响，按照纯化Hsp70 44-kD片段的方法对等物质量NDP激酶和Hsp70混合物进行酶切、纯化，检测得到的44-kD片段的ATP-ADP转换活性，确定Hsp70 ATP-ADP转换活性是Hsp70自身固有的，位于其N端44-kD片段处。

Objective The polysaccharides from fruiting bodies of Hericium erinaceus were isolated, purified, and characterized.

目的对猴头菇子实体中的多糖进行分离、纯化和组成分析。

Optimum Extraction of Ursolic Acid in the Residues of Eriobotrya japonica by Orthogonal Test;2. After total saponins of Panax notoginseng were extracted,residues left was refined,separated,and purified.

通过对三七总皂苷提取后的药渣进行重新提取、分离与纯化得到了三七中另一有效成分———三七多糖，经含量测定可达50%，提高了三七的再开发利用价值。

Results The five purified ginkgolic acid monomers were GA13:0.,GA15:0.,GA15:1 ,GA17:1 and GA17:2 ,with a side chain of 13,15,17 alkyl or ethylenic radicals respectively on their benzene loop.

结果纯化的银杏酸5个同系物单体为GA13：0、GA15：1、GA15：0、GA17：1和GA17：2，其母核苯环上分别有1个13、15及17烷基或烯基的侧链。

And then we applied the purified protein exogenously to bacterial cells, and their survival rate was observed.

将此蛋白质作用於不同细菌，测试作用不同时间下的细菌存活率。

4 Mg of the purified protein were obtained from 1 liter of bacterial culture.The results showed that rhCaM could extracellularly stimulate the proliferation of suspension-cultured cells of Angelica dahueica in the low density of initial cell culture.

每1 L菌液经Phenyl-Sepharose CL-4B 疏水亲和层析法纯化可获CaM纯品3～4 mg.rhCaM对白芷细胞增殖作用影响的研究结果表明，rhCaM在低细胞密度培养条件下对白芷细胞有促进增殖作用，效果高于标准植物CaM。

Results Astrocyte of rat optic nerve was cultured and purified,which showed positive reaction to glial fibrillary acidic protein.

组织块原代培养，振荡法分离纯化视神经的星形胶质细胞，对细胞的损伤小、纯度高，是快速得到该细胞的理想途径。

Genome, and its expression products are located in the flagellar, cytoplasm and fertilization tubule. Cr. actin is partially purified from Cr. cells by the method of assembly-disassembly-circle of actin.

我们利用肌动蛋白本身所具有的聚合和解聚的性质，从衣藻细胞中部分纯化了衣藻肌动蛋白，纯化的肌动蛋白能和抗兔α-平滑肌肌动蛋白的单克隆抗体发生专一性很强的免疫交叉反应。

Recall that, in the Kolmogorov axiomatization, measurable sets are the sets which have a probability or, in other words, the sets corresponding to yes/no questions that have a probabilistic answer.

延期开工的Kolmogorov宣布要衡量所有套职能有限许多坐标限制在于衡量子寅。换句话说，如果是/否的问题回答f可看的价值观念在许多最有限坐标，然后它有一个概率的答案。

The experimental results showed that the algorithm can filter singular points and remain fixity of the target edge.

实验表明：基于模糊理论的图像智能滤波方法比常规图像滤波方法运算量小，它既能去除奇异点，又能保持图像中目标的边缘不变性，具有很好的滤波效果。