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The intramolecular disulphide bridge was formed with iodine before the product was finally purified to homogeneity by reversephase HPLC.

产物经Sephadex柱层析、透析和RP-HPLC进行分离、纯化,最后鉴定其化学均一性和生物活性。

Fresh algal extracts prepared in 0.5 M HCl were neutralised, purified on a SPE column (RP-18, Merck), lyophilised in the presence of 1 mM dithiothreitol to prevent the formation of disulphide bridges and stored at -/20℃.

食藻提取物中拟定0.5米盐酸被瓦解,纯化的固相萃取柱(RP-18, Merck),lyophilised,在场的1毫米二硫苏糖醇,以防止形成二硫键桥梁和储存在-/20℃。

The purified cTnⅠ was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting.

纯化的cTnⅠ采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和免疫印迹法进行鉴定。

Our dustless enclosed air-conditioned workshop was designed and improved at the request and under the direction of many customers from the USA, Germany and Spain, and is a rare dustless and purified plastic packing workshop at home.

车间我公司的无尘封闭空调车间,是应美国、德国、西班牙等多家客户的要求及指导下设计,不断改进完善而建立,是目前国内少有的塑料包材无尘净化生产车间。

Experiments show that water solution contained 0. 5 -1 % sodium sulfate and 1 - 2% acetic acid is treated by means of electrodialyzer of 200 × 400mm assembled with tri - membrances and 19% sodium sulfate and 20% acetic acid are recovered by concentrating and separating. The content of salt and acid in the purified water is less than 0. 01%, current efficiency greater than 85 %, flow length in 3 ~ 5m and power consumption about 40 ~ 50 kwh/t.

实验表明,含0.5~1%硫酸钠、1~2%乙酸的水溶液通过三对膜组装的200×400mm电渗析器,分别得到含19%硫酸钠、20%乙酸的浓缩液,外排水中盐和酸的含量小于0.01%,电流效率大于85%,流程长度3~5m,耗电量40~50kwh/t。

Flammulin was purified to electrophoretic neity, and its characteristics are discussed for the first time.

火菇素被首次提纯至电泳纯,并首次叙述了它的理化性质。

The HBsAg in culture supernatant was purified by the same method, and their chromatographic and electrophoretic profiles were compared.

采用相同条件纯化培养上清中的HBsAg,比较二者的层析图谱及纯化抗原的电泳图谱。

Using NaCl and Na2HPO4 in turn to elute bound alkaline phosphatase, a 65-fold purified enzymatic preparation was obtained in one step with a yield of 89%.

用NaCl和Na2HPO4进行阶段洗脱,可使酶的纯化倍数一步达到65倍,活力回收率达到89%。

Alpha-fetoproteinhas been separated and purified from 3~5 months embryon by using salting out, gel chromatography and affinity chromatography.

甲胎蛋白(Alpha-fetoprotein,AFP)可从胎儿组织、胎儿心血、胎儿肝脏、羊膜液、脐带血清、肝癌患者血清和腹水等多种材料中分离获得,分离纯化方法国内外已有不少报道[1~6]。

It is demonstrated that the ATR difference spectroscopy of emplastrum is in good agreement with that of pure drugs purified by supercritical fluids extraction technique.

由差谱所得到的谱图与超临界流体萃取技术制备的纯有效成分谱图基本一致。

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昨晚我没有看电视,因为电视机坏了。

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