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Ferritin from the liver of a salt water fish, Dasyatis akajei, herein called DALF, was isolated and purified by thermal denaturation, ammonium sulphate fractionation and ammonium reverse extraction, Sephacryl S-300 gel filtration and DEAE- cellulose anion exchange chromatography.

通过热变性处理、硫酸铵分级沉淀、排阻层析和离子交换层析等技术，分离纯化了魟鱼肝铁蛋白Liver ferritin of Dasyatis akaiei。

TLC analysis demonstrated that highly purified HDC protein was able to decarboxylate histidine to form histamine Fig.

薄层色谱分析表明，HDC的高度纯化的蛋白质能够decarboxylate组氨酸形成组胺（图2bii）。

There is also the message of a defiled people being further purified and blessed.

这里也有污秽的人民被洁净和赐福的信息。

METHODS: The model of tissue damaged submandibular gland in 10 rats was made by deligation. One week later, the gland tissue was obtained to harvest submandibular gland stem/progenitor cells by enzyme digestion in vitro. Following 10-14 days of primary culture, small round cells were collected, purified and subcultured for monoclonal culture.

10只大鼠采用结扎下颌下腺主导管、抑制腺体分泌来建立组织损伤模型，1周后切取腺体组织，酶消化法体外分离下颌下腺干/祖细胞，原代培养10~14 d后，挑取培养皿中形成的小的类圆形、类上皮细胞集落予以纯化，传代后进行单克隆培养。

The fungi was isolated and purified from polluted anthurium subculture medium, identified to be Deuteromycotina Hyphpmycetes Hyphomycetales Dematiaceae and trichoderma. The inhibition effect of the three kinds of chitosan on the fungi was conducted.

在污染的红掌继代培养基中提取污染菌并进行了分离及纯化，初步鉴定为为半知菌亚门，丝孢纲，丝孢目，淡色孢科，木霉属。

During the incubation of purified α-synuclein with fructose or glucose, it was observed that the protein intrinsic fluorescence at 308 nm decreased while the fluorescence of glycated derivant at 447 nm increased.

将纯化后的α-synuclein分别与果糖和葡萄糖孵育，通过内源荧光、非酶糖基化衍生物特征荧光、圆二色光谱以及电子显微镜等技术进行检测发现：α-synuclein 与还原糖共同孵育后，308 nm内源荧光强度明显降低，同时在447 nm产生一个非酶糖基化衍生物特征荧光。

By n-butanol extraction, ammonium sulfate fractionation and sodium chloride dialyzation, the partially purified enzyme was gained by means of gel filtration on sephadex G-150 column, and some properties of the enzyme were determined.

方法］以DFRKN-1为提酶材料，经正丁醇提取，硫酸按分级沉淀分离，透析获得酶的粗提取液，用SephadexG-150凝胶过滤柱层析纯化，获得纯化碱性磷酸酶，并测定纯化碱性磷酸酶的理化性质。

The purified TGF β1 monomer protein can form into active dimeric TGF β after refolding in vitro.

纯化的TGFβ1单体蛋白通过体外再折叠可以形成具有生物活性的TGFβ1双体分子。

The purified BMMSCs with ability of multiplication could be obtained and induced directionally into endothelium like cells in vitro.

结论体外可以获得足够量的较为单一、具有较强增殖能力的间充质干细胞，而间充质干细胞可以在体外定向分化为具有内皮血管细胞特性的细胞。

The water for transfusion injection is produced by using multi-effect water distillatory, namely to adopt repeatedly heat exchange to distill purified water, and to meet the standard of injection water.

大输液注射用水采用多效蒸馏水机生产，即通过多次热交换对纯化水进行蒸馏，以达到注射用水水质标准。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。