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In this case the sound, which emerges beyond the framework of frequency band, which corresponds to human voice, is filtered, and user hears against the background music purified and improved speech of collocutor.

在这种情况下，健全的，其中出现超越的框架内，频带，相当于人类的声音，过滤，用户听到反对的背景音乐，纯化和改进的讲话collocutor 。

Firstly, MiCy, Citrine, mKO were inser into expression vectors of pRSETB, and then MiCy, Citrine,mKO were expressed in E.coli and purified by using Ni-NTA collum chromatography.

首先，构建了含有六个组氨酸标签的三种荧光蛋白MiCy、Citrine和mKO，而后在大肠杆菌中表达了MiCy、mKO和Citrine三种蛋白，并用金属鳌合亲和层析进一步纯化，纯化产物使用特异性酶去除His-tag。

Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.

本论文在上述研究成果的基础上，选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型，进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制，探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制；以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制；以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响，从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。

By a method using fraction precipitation of ammonium sulphate, ion exchange, modified hydrorylapatite and gel filtration chromatography, we separated and purified two kinds of phycoerythrin(R-PE and r-PE) from Corallina officinalis . The purity standard(ratio of ODs65/OD2go) of R-PE reaches to 11, which is the highest purity comparing with the reported purity of phycoerythrin.

经盐析、离子交换层析、羟基磷灰石吸附层析和凝胶过滤层析等分离纯化方法，从珊瑚藻中分离纯化出两种藻红蛋白，其中R-PE纯度OD_(565／OD_(280)高达11左右，是目前国内外报道的藻红蛋白中纯度最高。

Using SPDP as a heterobifunctional crosslinker, R-PE isolated from Corallina officinalis and sheep anti-rabbit IgG purified by ion exchange chromatography as conjugated reagent, we prepared the phycoerythrin fluorprobes by two-step binding protocol.

以双功能试剂SPDP为交联剂，从珊瑚藻中纯化的R-PE和购买的离子交福建农林人学博1：学位论文2二换纯化的羊抗兔抗体为标记材料，利用两步标记法获得藻红蛋白抡疫荧光探针。

Methods: The olfactory nerve layer and glomerular layer was isolated from the olfactory bulb of three-day old rats, Enzyme digestion method was used to isolate cells and the cells were purified by differential adhesion method. Freshly isolated OECs were plated onto poly-L-lysine-coated tissue culture and then incubated for 2 days. The purity of OECs was identified by immunocytochemistry of NGFRp75 and S100 and Hoechst33342 counterstain method.

从新生SD大鼠(3d)嗅球中迅速分离嗅神经层和嗅颗粒层，采用酶消化法分离细胞，差速贴壁法纯化细胞，接种于多聚赖氨酸包被的培养板内培养2d，采用NGFR p75和S100蛋白双标免疫组化、以Hoechst33342复染鉴定OECs的纯度。

By use of AB-8 macroporous resin and with 10％ gardenia yellow as adsorptive solution, selecting proper adsorptive and desorptive conditions, crocin and geniposide can be separated effectively, the purified gardenia yellow whose colority increases from 10 to 320 and OD ratio decreases from 2. 31 to less than 0.4 answers for the standard of gardenia yellow of high quality.

采用AB—8大孔树脂，以10％的栀子黄溶液上柱，选择合适的吸附、解吸条件，栀子黄的色价可从10提高至320，OD值比率也从2.31降至0.4以下，藏花素和栀子甙均达到了有效地分离。

Results:The cytolytic activity of fresh NK cells and purified NK cells from LAK cells was highly increased,but that of CD3AK cells was lowered.The cytotxicity of LAK cells was not affected.

结果：外周血新鲜NK细胞和纯化NK细胞对K562的杀伤率明显提高，CD3AK细胞的杀伤力明显降低，LAK细胞的杀伤无明显变化。

In this research, the isolates of TuMV from Shandong were separated and purified. Their biological, serological, cytopathological and particle morphological properties were studied.

本研究分离纯化了芜菁花叶病毒的山东分离物，对其生物学、血清学、细胞病理学和粒子形态学特征进行了研究。

Cytophaga sp was selected for heparinase production because it was more productive. Three heparinases, named HⅠ, HⅡ and H Ⅲ were purified from the crude cell extract by means of batch DEAE-Cellulose negative adsorption, Hydroxylaptide adsorption and releasing, SP-Sepharose cation exchange chromatography, Source 30S cation exchange chromatography, Poros 20HS cation exchange perfusion chromatography and Sephacryl S200HR gel permeation chromatography.

利用超声破碎、DEAE-纤维素反吸附去杂、羟基磷灰石批处理吸附层析、SP-Sepharose离子交换层析、Source 30S离子交换层析、Porous20HS离子交换灌注层析及Sephacryl S200HR凝胶排阻层析，从噬纤维菌中纯化出三种新的肝素酶。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。