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Acid phosphatase from Branchiostoma beleheri was isolated and purified by ammonium sulfate fractionation,Sephadex G-75 gel filtration and CM-cellulose chromatography under acid condition.

从厦门文昌鱼Branchiostoma belcheri分离提纯酸性磷酯酶(EC3.1，3.2)，进一步经Sephadex G-75和羧甲基纤维素(CM-52)柱层析纯化，获得聚丙烯酰胺凝胶电泳单一纯酶制剂，比活力为351μm/min mg。

The MTT assay was used to detect the viability of cells. Flow cytometry and laser confocal microscopy were used to identiyfy the cell cycle and apoptotic morphology after cells treated with LAO. RESULTS: An L-amino acid oxidase was successfully purified from the venom of bungarus fasciatus.

结果： 通过两步层析法从金环蛇毒中分离得到L-氨基酸氧化酶，L－氨基酸氧化酶的表观分子量通过SDS-PAGE测定约为60 kD，通过凝胶过滤测定分子量为70 kD.L－氨基酸氧化酶作用于HUVCE细胞12 h后，细胞的生长明显受到抑制，L－氨基酸氧化酶作用的半数抑制量为2.8 mg/L。

Copper-zinc superoxide dismutase was purified from Canarium album by ultrasonic, precipitation with ammoniurn sulfate,Sephadex G-100 filtration and DE-52 chromatography.

采用超声波破碎、硫酸铵分级沉淀、Sephadex G-100和DE-52柱层析，从橄榄中分离纯化Cu，Zn—SOD，并对其部分性质进行分析鉴定。

Water Purified, Aloe Vera, Vegetable Glycerin, Hi-olec Canola Oil, Geranium, Stearic Acid, Caprylic/Capric Triglycerides , Cetyl Esters, Camellia Oil, Vitamin C, Vitamin A, Our Proprietary Essential Oil Blend, Our Proprietary Broad Spectrum Preservative System* organic or sustainable

净化水，芦荟，蔬菜甘油，高olec菜籽油，天竺葵，硬脂酸，辛酸/癸甘油三酯，烷基酯，山茶花油，维生素C，维生素A，我们独有的精油配方，我们的专有广谱防腐剂系统*

Objectives To improve the purity and safety of TAT immunoglobulin F2 purified by caprylic acid.

目的 为了更进一步提高F2的纯度和抗毒素的安全性。

The results indicated that the purity and immunoactivity varied according to different purification methods. IgG purified by caprylic acid precipitation plus ammonium sulfate precipitation is the best for further use with 98.05%purity and 1800 ratio activity.

结果表明，不同方法纯化的抗体其纯度和免疫学活性具有一定程度的差别，其中经辛酸+饱和硫酸铵沉淀纯化的抗体为最佳，凝胶扫描纯度为98.05%，比活性近1 800，为纯化前的6.8倍。

Purified water, euphorbia cerifera wax, glyceryl stearate, ammonium acrylates copolymer, butylene glycol, copernica cerifera wax, stearic acid, sucrose acetate isobutyrate, acrylates copolymer, bentonite, tromethamine, hexylene glycol, silk amino acids, cholesterol, glycine, synthetic wax, ethylhexyl glycerin, alanine, PEG-9 dimethicone, nylon-6, lecithin, silica, simethicone, caprylyl glycol, xanthan gum, propylene glycol, isostearic acid, sodium laureth-12 slfate, ceteareth-20, polyvinyl alcohol, sodium chloride, sodium acetate, disodium EDTA, benzyl alcohol, chlorphenesin, phenoxyethanol, methylparaben, ethylparaben, propylparaben, butylparaben, isobutylparaben, may contain /- mica, iron oxides (CI 77491, CI 77492, CI 77499), titanium dioxide (CI 77891), ferric ferrocyanide (CI 77150), ultramarines (CI 77007), blue 1 lake (CI 42090), bronze powder (CI 77400), aluminum powder (CI 77000), bismuth oxychloride (CI 77163), carmine (CI 75740), chromium hydroxide green (CI 77289), chromium oxide greens (CI 77288), yellow 5 lake CI 19140

水，小烛树蜡，硬脂酸甘油酯，丙烯酸铵共聚物，1，3-丁二醇，巴西棕榈蜡，硬脂酸，异丁酸醋酸蔗糖酯，丙烯酸酯共聚物，蒙脱土，二吡喃乙酸乙酯胺，已烯醇，丝氨酸，胆固醇，甘油，合成蜡，异丁基甘油，丙胺酸，PEG-9二甲基硅油，尼龙-6，卵磷酯，硅粉，硅氧烷，癸酰基乙二醇，汉生胶，丙二醇，异硬脂酸，月桂基醚-12硫酸钠，十六十八醇醚-20，聚乙烯醇，氯化钠，醋酸钠，EDTA二钠，苯氧乙醇，氯代苯酚，卞醇，尼泊金甲酯，尼泊金乙酯，尼泊金丙酯，尼泊金丁酯，尼泊金异丁酯，[可能还含有云母，铁黑(CI 77491， CI 77492， CI 77499)，钛白粉(CI 77891)，氰化亚铁(CI 77150)，靛蓝(CI 77007)，蓝1号(CI 42090)，青铜粉(CI 77400)，铝粉(CI 77000)，氯氧化铋(CI 77163)，洋红色素(CI 75740)，氢氧化铬绿(CI 77289)，氧化铬绿(CI 77288)，黄5号(CI 19140)]文章来自网络，不代表本网站立场，版权归原作者所有，转载请注明出处！

This experiment with dilute alkali swelling on collagen extraction and separation, then purified by gel from collagen, and then glutaraldehyde, carbodiimide, has three types of di-isocyanate cross-linking agent on the plastic recombinant protein molecules cross-linked, the final test of infrared spectroscopy analysis of the results of their cross-linking to determine the extent of the effectiveness of cross-linking cross-linking agent to have an initial understanding.

本实验用稀碱溶胀法对胶原蛋白进行提取与分离，接下来用凝胶层析纯化所得胶原蛋白，然后用戊二醛、碳化二亚胺、己二异氰酸酯三种交联剂对胶原蛋白分子进行重组交联，最后通过对红外光谱测试结果的分析，对其交联程度进行判断，对交联剂的交联功效有一个初步的了解。，谢谢。

Ion-exchange chromatography with DE-52 was used for the purification of the antigens. The purified products were characterized by Western-blotting. Result For the pollen extract of Caryota ochlandra, 30 protein bands were detected in SDS-PAGE.

收集过敏病人血清，采用免疫印迹(Western-blotting)法鉴定其变应原成分，通过离子交换层析对花粉变应原进行初步纯化和免疫印迹鉴定。

The physical and chemical properties of the purified MPH were studied. In the crude extracts, the specific activities of MPH for methyl parathion and fenitrothion were higher than those of OPH from F.sp, while the catalytical activity for parathion was lower than that of OPH.

我们根据Pseudomonas sp WBC-3的生长特性及产酶情况，以阳离子交换层析纯化胞内粗蛋白中，使酶的比活提高了49.1倍，得到了均一的目的蛋白MPH，SDS-PAGE验证为单一的条带，。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。