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Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖；(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制：采用SLP试剂盒对提取物定性检测，应用动态浊度法鲎试验测定提取物中内毒素含量，采用改良LOWRY试剂检测提取物中蛋白的含量；(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件：青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

In addition a simple process was also developed to obtain purified betulin using chloroform / NaOH two-phase solvent extraction.

另外还建立了一种在碱性条件下用氯仿/水溶液混合溶剂来一步纯化白桦树皮中的白桦酯醇的方法。

In this thesis betulin was purified by recrystallization with chloroform and methanol mixture, and orthogonal design had been taken to optimize the conditions of recrystallization including the volume and ratio of chloroform and methanol mixtures.

在白桦酯醇的纯化方面目前比较成熟的方法是氯仿+甲醇混合溶剂重结晶来纯化白桦酯醇，本文通过正交设计，得到最优化的溶剂比例和用量。

Purified MBP-Ce-ArsA-1 fusion protein was biochemically characterized, and its properties were compared with those of E.

elegans中具有两个ArsA蛋白的同源体，我们发现藉由RNA干扰技术抑制Ce-ArsA蛋白的产生，将会造成C。

The crude protein was obtained by extracting the leaves of Blechnum orientale with PBS and precipitating with (NH4)2SO4. The crude protein was purified by Sephadex G－100 filtration after treatment through active carbon column.

乌毛蕨的叶片经PBS抽提和硫酸铵沉淀，得到的粗蛋白经活性碳柱处理后，在分子筛层析柱Sephadex G-100上纯化得到一种凝集素（Blechnum Orientale Lectin，简称BOL）。

Terpenoids were separated and purified from Bletilla striata by extraction and chromatography.

采用萃取和色谱法从白芨中分离和纯化了该萜类化合物。

Two types of protists Bodo and Gymnodinium were isolated, purified, and structurally confirmed.

用微量分离法分离出两类主要的原生生物。

It is the first time to clone the BiVP gene from the bumblebee, Bombus ignitus and express it in Spodoptera frugiperda Sf-9 cell. The purified recombinant protein fulfilled the needs of further studying the bioactivity of this protein.

首次在红光熊蜂中克隆到蜂毒蛋白酶基因，在昆虫杆状病毒表达系统中进行了表达，并对表达产物进行了纯化，为深入研究BiVP的生物活性奠定了基础。

Methods The BiVP gene was screened by the ESTs technology and cloned by PCR method from the cDNA library of the bumblebee, Bombus ignitus. It was reconstructed to form the baculovirus transfervector pBac1-BiVP and expressed in Sf-9 insect cell. The recombinant BiVP was purified by the fast protein liquid chromatography system.

利用ESTs从红光熊蜂cDNA文库中获得BiVP基因，以PCR技术扩增出BiVP的完整cDNA，并构建病毒表达载体PBac1-BiVP，通过昆虫杆状病毒表达系统，在Sf-9昆虫细胞中进行表达，表达产物用快速蛋白液相色谱系统进行纯化。

Zhuhai Boyang Technology Co., Ltd is a professional antistatic, purified products supplier. We own a team of experienced engineers and skilled production workers. With our professional skill and honesty, we offer you a complete good service. We honor contracts and maintain commercial integrity.

珠海博扬电子科技有限公司是一家经营防静电、净化产品的专业生产供应商，拥有一批经验丰富的技术人才、高素质的管理人才和营销精英，以专业的技术水平和诚信的经营态度竭诚为广大客户提供全面、优质的服务。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。