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After cultivating in liquid medium for 5 days, an extra cellularβ-mannanase from Athelia rolfsii strain CBS191.62 was purified 15.1-fold toelectrophoretic homogeneity by ammonium sulfate precipitation, DEAESepharose Fast Flow chromatography, Hydroxylapatite chromatography andrefrigeration crystal.

产β-甘露聚糖酶的Athelia rolfsii菌株CBS 191.62，发酵培养5d，发酵液离心去菌体，上清经硫酸铵沉淀，琼脂糖凝胶层析、羟基磷灰石层析和冷冻干燥结晶等步骤，β-甘露聚糖酶的比活提高了15.1倍，获得凝胶电泳均一的蛋白样品。

Purified recombinant protein rCCR5 isobtained successfully, which can be used as a candidateantigen for CCR5 autovaccine to challenge HIV1infection.

获得了大量纯化的rCCR5重组蛋白，可作为研制CCR5自体蛋白疫苗以阻断HIV1感染的候选抗原蛋白。

purified recombinant protein rccr5 is obtained successfully, which can be used as a candidate antigen for ccr5 autovaccine to challenge hiv 1 infection.

获得了大量纯化的rccr5重组蛋白，可作为研制ccr5自体蛋白疫苗以阻断hiv 1感染的候选抗原蛋白。

Coding sequence of pig adiponectin was digested with restriction endonuclease Aval, 267bp fragment of N terminus and 372bp fragment ofC terminus were purified and ligated.

用限制性内切酶Ava Ⅰ处理脂联素编码区，回收N端267bp和C端372bp片段，连接，然后进行PCR扩增，从而得到竞争性模板。

GC were applied to study the component sugars of a polysaccharideisolated and purified from aweto.

采用气相色谱法研究自虫草菌中分离、化的多糖的单糖组成。

Employing capillary-pipette washing method,the unialgal strain and axenic strain of Microcystis aeruginosa from Songhua Lake were isolated and purified,and effects of temperature,illuminance,nitrogen,phosphorus,and iron on their growth were investigated.

用毛细滴管洗净法从松花湖分离纯化出铜绿微囊藻的单藻株和无菌株，研究了温度、照度、N、P、Fe各种因素对单藻株和无菌株生长的影响。

Here, we presented a method to bacterially express the major structural protein L1 of Human Papillomavirus type 18 (HPV18) as soluble form. We found that the purified L1 could self-assemble to virus-like particles.

利用大肠杆菌表达系统可溶性表达人乳头瘤病毒18型(HPV18)L1蛋白，经过纯化和重组装过程获得HPV18病毒样颗粒，研究其免疫原性和诱发中和抗体生成的水平。

Methods TGGP was purified from Balanophora japonica Makino by n-hexane and ethyl acetate extraction and column chromatography.

用萃取和柱层析方法分离提纯TGGP，用酶联免疫测定法、天然聚丙烯酰胺凝胶电泳、分子排阻高效液相色谱法检测其抑制HIV gp41六螺旋束结构形成的作用，用细胞－细胞融合方法检测TGGP抑制HIV进入靶细胞的活性。

To understand the sanitary status of barreled purified water, to Guarantee the quality and safety of water supply.

目的 了解无锡市桶装饮用纯净水卫生状况，以保证供水的质量和安全。

Trypsin inhibitors, the major root storage protein of sweet potato, were purified from different cultivars of Tainong 57 and Tainong 65 of sweet potato Ipomoea batatas

中文摘要胰蛋白酶抑制因子，为甘薯块根中主要储藏性蛋白质。本研究以trypsin-Sepharose 4B 亲和管柱层析方法，从台农57号甘薯块根和台农65号甘薯 Ipomoea batatas

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。