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Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

ABSTRACT To study the antibacterial constituents from the fermentation broth of endophytic fungus Penicillium sp. 0935030 from mangrove plant Acrostichum aureurm. The separation procedure was guided by antibacterial bioassay. Seven compounds were isolated and purified by column chromatography, and their structures were identified as cyclo (1), cyclo (2), 1(2Furanyl)1,2ethanediol (3),(3β,4β,22β)olean12ene3,22,23triol (4), mannitol (5), uridine (6), and liquiritigenin (7) by physicochemical and spectral data.

为研究红树林植物卤蕨内生真菌Penicillium sp.0935030发酵物中的抗菌活性成分，采用活性追踪的方法，用多种色谱技术对化合物进行分离纯化，通过理化性质和光谱数据鉴定出7个化合物，分别为：环(1)，环(2)，1呋喃基1，2乙二醇(3)，(3β，4β，22β)12烯3，22，23三羟基齐墩果烷(4)，甘露醇(5)，尿苷(6)和甘草素(7)。

Seven major bacterial isolates were obtained and purified. Their 16S rRNA genes were amplified by PCR for sequencing and their identities were determined with homology comparisons. Five of the seven isolates belong to Actinomycetales including Kocuria KD139, Rhodococcus KD140, Rhodococcus KD142, Arthrobacter KD230, and Arthrobacter KD232; one is classified as Bacillus d KD178; and another one as Stenotrophomonas KD237. The phylogenetic tree was also constructed based on the analysis of 16S rRNA gene sequences.

通过对分离菌株的16S rRNA基因序列进行分析，发现其中5株细菌分别属于放线菌目的考克氏菌属(KD139)、红球菌属(KD140和KD142)和节杆菌属(KD230和KD232)， 1株细菌属于杆菌目的芽胞杆菌d属(KD178)，另外1株细菌属于黄色单孢菌目的寡食单胞菌属(KD237)；同时我们构建了系统进化树，确定分离菌株的相对进化地位。

The fusion protein was purified through Glutathione-Sepharose 4B affinitive chromatography and GST part was cut off with thrombin.

然后经Glutathione-Sepharose 4B亲和纯化后，用凝血酶切除GST部分。

AIM: To investigate the characteristics of a novel anti-platelet protein, agacutegrin, which was purified from Agkistrodon acutus.

目的： 分离纯化尖吻蝮蛇毒中抗血小板聚集活性蛋白agacutegrin，并研究其生化性质。

Polyhedrin and virion, which were isolated and purified from Agrotis setegum nuclear polyhedrosis virus, are administered to 3-instar Heliothis armigera larvae with Heliothis armigera nuclear polyhedrosis virus. Both polyhedrin and virion could increase mortality of H. armigera larvae, shorten half mortality time and decrease half mortality concentration.

对黄地老虎核型多角体病毒的多角体蛋白和病毒粒子组分进行分离纯化，分别与棉铃虫核型多角体病毒混合感染三龄棉铃虫幼虫，发现此二种组分均能够提高棉铃虫幼虫的死亡率，缩短半致死时间，降低半致死浓度。

RNA was extracted from SIV which was purified from infected allantoic fluid, and expected band was observed via RT-PCR.

从纯化的病毒中提取RNA，进行RT-PCR，可扩增出预期的条带。

Methods Viruses were passed in embryonated hen eggs, and virion RNA was extracted from allantoic fluid and reverse transcribed to synthesize cDNA. cDNA was amplified by PCR and the PCR product was purified with a purification kit.

方法病毒在鸡胚中传代，从收获的尿囊液中提取病毒粒RNA，通过逆转录合成cDNA.cDNA通过PCR进行扩增，接着PCR产物用纯化试剂盒进行纯化。

Firstly , the V4 stock was purified three times by plaque formation. It's difficult to see clear plaques in CEF cells due to the avirulent characteristic , so we treated the V4 stock with trypsin and then allantoic Fluid and Mg2+ were added in the layer gel. In this case, we could see small plaques occasionally, but not stably.

本实验首先对实验室保存的V4株进行了三轮的蚀斑纯化，由于V4株属无毒株，其在CEF细胞中很难形成可见的蚀斑，因此我们采用了用胰酶处理V4病毒，并在上层胶中添加尿囊液及Mg2+，这样可以偶尔产生可见的蚀斑，但实验结果重复性很差。

The avian influenza virus RNA was directly extracted and purified from chicken em-bryo allantoic fluid. According to published gene sequence,a pair of specific primers to the nucleoprotein gene of AIV was designed and synthesized. After that,the NP cDNA was amplified by RT-PCR .Then the complete NP gene was sequenced.

直接从鸡胚尿囊液中提取H9N2亚型禽流感病毒的RNA，并根据已发表的A型流感病毒株的核苷酸序列，设计了1对特异引物，采用RT-PCR技术成功地扩增了AIV的NP基因；将NPcDNA克隆后进行了序列测定。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。