查询词典 purified

Objective To study the diagnostic value of tuberculosis protein chip,acid-fast smear and tuberculin purified protein derivative in pulmonary tuberculosis.

目的探讨结核蛋白芯片、痰涂片抗酸染色及结核菌素皮试单独和联合检测对肺结核患者临床诊断的意义。

Objective To explore the response intensity to tuberculin skin test of purified protein derivative in different populations and the long-term effect of isoniazid chemoprophylaxis on strong positive reactors.

目的探讨不同的人群对结核菌素皮肤试验的反应强度以及对强阳性反应者采用异烟肼化学预防的远期效果。

Methods Balb/c mice were immunized peritoneally by purified protein from tuberculin ? derivatives.

用PPD免疫Balb/c小鼠，胸腺切片用免疫组织化学法处理，光镜下观察。

OBJECTIE: Tuberculin skin testing with purified protein deriatie is part of tuberculosis T screening in patients receiing infliximab.

目的：用精制结核菌素进行结核菌素皮肤试验是接受infliximab患者进行结核病检查的一部分。

He answered him,＂ For two thousand three hundred evenings and mornings; then the sanctuary shall be purified.

那说话的回答他说：＂要延长二千三百昼夜，以后圣所再要恢复原状。

The virus was purified by ultracentrifuge.

根据AIV基因组特点设计1条通用引物T-1，反转录获取cDNA。

The virus was purified by ultracentrifuge. According to the NS gene sequence published by Genbank, One primer T-1 was designed to amplify the cDNA by reverse transcript.

本实验用接种鸡胚的病毒增殖方法获得了A/Chicken/Mudanjiang/0823/00(H9N2)分离株的大量增殖，增殖病毒经差速离心进行纯化，经超迷离心浓缩。

Methods The HPV16E7 gene was cut to three parts, amplified by PCR, and connected individually with BPVL1 on plasmid PUC. With PVL1393 as a transfect vector, the BPVL1/HPV16E7 recombinants were transfected to baculovirus which subsequently expressed the chimeric BPVL1/HPV16E7 protein in the SF－9 cells. The recombinant proteins were then purified by centrifuge, sonication, sucrose and CsCl ultracentrifuge, and were identified by SDS-PAGE, Western blot, ECL and TEM.

HPV16E7基因分3段经PCR扩增后分别克隆入连有BPVL1的质粒PUC形成BPVL1-HPV16E7；以质粒PVL1393为载体将BPVL1-HPV16E7基因转染杆状病毒并在昆虫细胞中进行表达；用超声粉碎和蔗糖超离、氯化铯梯度离心等方法纯化以及免疫印迹、ECL、透射电镜等方法鉴定表达产物。

Adenovirus 5第二军医大学博士学位论文英文摘要胸心外科专业(WAd5) and PDC-MLC-VEGF were transferred to the adenoviral packaging cell HEK 293cell by lipofectamine 2000 mediated gene transfer method to pack the virus; The desired Advectors were purified by density gradient ultracentrifuge and titrated in 293 cells afteridentified.

Ad.mlc-hVEGF165 体外转染心肌细胞的实验研究分离培养乳鼠心肌细胞，在同一 MOI 值下，Ad.mlc-hVEGF165 和 Ad.hVEGF165分别转染心肌细胞，以 RT-PCR 法和 Western 印迹分析法分别检测转染后心肌细胞内VEGF mRNA 的转录及 VEGF 蛋白的表达，以 ELISA 法检测转染后心肌细胞分泌的VEGF 蛋白质，MTT 等方法观察其对促内皮细胞增殖作用。

To study the effect on the content of cholesterol,triglyceride and high density lipopotein in serum,the soluble dietary fibers extracted from soybean residue and soybean hull with phosphate buffer and purified with the ultrafilter was fed to Kun Ming Species mouse.

研究了以磷酸盐缓冲溶液提取-超滤纯化法从大豆渣、大豆皮中提取的大豆膳食纤维对血清胆固醇、甘三酯水平和高密度脂蛋白水平的影响。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。