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Multiwall carbon nanotubes attached on conventional AFM silicon tip using an optical microscope with two independent translation tables. Purified nanotubes are aligned on the conductive tape, and attached on the AFM tip.

首先将碳纳米管粘附在导电的胶带上，然后用涂胶的针尖与其接触将碳纳米管粘附到针尖上，最后运用电蚀的方法优化碳纳米管针尖的长度，以达到高分辨率的要求。

Methods The phage displayed antigens of paragonimus were used to examine the sera of patients with paragonimiasis,schistosomiasis,trichinosis and healthy people,and their sensitivity and specificity were acquired;The templates of the mimic antigen were purified to determine the sequence and the sequence homology was analyzed by Blast.

方法建立肺吸虫噬菌体模拟抗原的ELISA，对肺吸虫病人、日本血吸虫病人、旋毛虫病人及健康者血清进行检测，分析其敏感性和特异性；提纯噬菌体模拟肽的DNA模板进行序列测定，以Blast软件分析其序列同源性。

Triose phosphate isomerase was prepared and purified from chicken breast muscle. The equilibrium unfolding of TIM induced by guanidine hydrochloride was investigated by following the changes in intrinsic fluorescence, circular dichroism and second-derivative spectroscopy.

从鸡胸肌中纯化出磷酸丙糖异构酶(triose phosphate isomerase， TIM)，通过蛋白质内源荧光，圆二色性，紫外吸收二阶导数光谱等多种研究溶液构象的方法，对TIM被盐酸胍和热变性过程进行了详细的研究。

Methods The antimicrobial peptide of M. domestica pupa were produced largely by infection and injury, which were isolated and purified by trituration, centrifugalization and column chromatography of AKTA purifier. Then the antimicrobial peptides with anti-Toxoplasma activity were selected by haemacytometry and MTT colorimetric method.

通过损伤加感染的方法诱导家蝇蛹大量表达抗菌肽，然后经过研磨、离心和AKTA purifier层析等步骤，将家蝇蛹抗菌肽进行分离纯化，采用血细胞计数法和MTT比色法筛选对弓形虫速殖子有抑制作用的抗菌肽。

Methods The antimicrobial peptides of housefly larvae were induced largely by infection and injury, which were isolated and purified by trituration, centrifugalization and column chromatography. Then the antimicrobial peptides with anti-Toxoplasma activity were sieved by methyl thiazolyl tetrazolium colorimetric method and haemacytometry. The DNA contents of T.gondii tachyzoites were detected by flow cytometry.

通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，分离纯化并筛选出抗弓形虫作用的抗菌肽，采用流式细胞术分析其对弓形虫速殖子DNA含量的影响。

objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.

作者单位：山西医科大学寄生虫教研室，太原 030001；化学生物学与分子工程教育部重点实验室山西大学生物技术研究所目的从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，将家蝇幼虫抗菌肽进行分离纯化，采用四甲基偶氮噻唑蓝比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经resource s阳离子柱和superdex g75凝胶柱层析后，筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽。结论家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽，而且不止一种，但其效果有所不同。

The infection rate was significantly decreased if we upregulate Attacin expression by E.coli injection prior to trypanosome feeding.Attacin gene was cloned and expressed in Drosophilla S2 cells. Purified protein shows strong activity against E.coli, as well as trypanosome. But tsetse symbiont Sodalis, a gram negative bacterium, was quite resistant to Attacin.

克隆Attacin基因并在果蝇S2细胞中表达，对纯化的Attacin蛋白的研究表明它除了对大肠杆菌有很强的活性外，对锥虫也有较强的活性，而同为格兰氏阴性菌的采采蝇共生菌Sodalis则在试验中表现了较强的抗性。

objective to study a modified method for detecting inhibitory activities of trypsin inhibitors purified from momordica charantia seeds.

目的用一种改进的测定方法，测定从苦瓜籽中提取分离的胰蛋白酶抑制剂的抑制活性。

The experiment extract The trypsin used the way of the trypsinogen activated by self, then, according to the characteristic of hydrolysis determinated the enzyme activity. at last,use the method of salting out purified The trypsin.

本实验采取胰蛋白酶原自身激活的方法提取胰蛋白酶，再根据胰蛋白酶的水解特性，采用酪氨酸测定酶活，最后根据盐析的原理，提纯胰蛋白酶。

METHODS Using immunohistochemistry method, we observed the Fos expression in the brain of purified protein derivative of tuberculin immunized mice at different time points.

用免疫组织化学方法观察纯化的结核菌素衍生蛋白免疫刺激后不同时间点小鼠脑内的 Fos蛋白表达。

The X-ray diffract was used to analyze the crystal structure of the composite. Results show that after the talc was treated with blend coupling agent, the crystal structure of talc changed, and effect Intercalation phenomenon.

通过X射线衍射分析可知，用混合偶联剂对滑石粉进行预处理后，滑石粉晶体结构发生改变，产生了插层现象，使PP／滑石粉复合材料为纳米复合材料。

An authentic Maui wedding might require the services of a 'kahu', which is a Hawaiian minister.

毛伊可能需要一个真实婚礼服务'kahu'，这是夏威夷部长。