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Shark Hepatic Stimulator Substance was extracted and purified from healthy Squalus mitsukurii.

我们从健康长吻角鲨肝脏中分离纯化得到了鲨鱼肝刺激物质(Shark HepaticStimulator Substance，sHSS)。

Methods: Rat MSC was isolated and purified in vitro,labelled by Hoechst33342, stereotaxis imbed into CA4 of rat hippocampus.

近来研究发现，MSC可以分化为神经元样细胞、胶质样细胞等［1］，植入体内后对脑和脊髓的损伤性疾病有治疗作用［2］，为MSC替代治疗神经系统疾病提供了实验基础。

Methods: Rat MSCs was isolated and purified in vitro, labelled by Hoechst33342, stereotaxis imbed into CA4 of rat hippocampus. To kill rat and recipe brain after 1,2,4 weeks to be made to frozen section, amd to observe Hoechst33342-marked cells under fluorescence microscope.

分离大鼠MSCs体外培养、纯化，Hoechst33342标记后立体定向植入大鼠海马CA4区，1、2、4周后处死大鼠取脑，制作冷冻切片；用荧光显微镜观察Hoechst3342阳性细胞。

Results Eleven compounds were purified and their structures were identified, they were identified as β-sitosterol, stigmasterol, nonacosanyl caffeate, daucosterol, betulinic acid, vanillic acid, inositol methyl ether, sucrose, soyasaponin Ⅰ, kaikasaponin Ⅲ, and dehy-drosoyasaponin Ⅰ.

结果 分得11个已知化合物，经鉴定为β-谷甾醇、豆甾醇、咖啡酸二十九醇酯、胡萝卜苷、白桦酸、香草酸、肌醇甲醚、蔗糖、soyasaponin Ⅰ、kaikasaponin Ⅲ和dehy-drosoyasaponin Ⅰ。

The structure and morphology of CNTs which have been purified and not yet, were characterized by TEM, SEM, XRD, Raman, TG-DTA, BET, IR. The results indicated that when the stokehole reaction temperature was 1170℃,the decompose temperature of Ferrocene was 150℃,he volatilize temperature of Benzene was 50 ℃, hydric flow rate was 270ml/min, the concentration of thiophene was l% and the reaction time was 20 minutes, the CNTs with single-walled and multi-walled were prepared.

结果表明：炉膛反应温度1170℃、二茂铁的分解温度150℃、苯的挥发温度50℃、氢气的流量270ml／min、噻吩体积分数1.00／100ml苯、反应时间20min时，可以得到碳纳米管，既有单壁管又有多壁管，单壁管的内径分布在0.88～1.15nm之间，多壁管的直径分布在40～120nm之间；纯化后的碳纳米管比表面积增加，管壁光滑，而且产生了大量的含氧官能团，有利于在水溶液中的分散。

The structure and morphology of CNTs which have been purified and not yet, were characterized by TEM, SEM, XRD, Raman, TG-DTA, BET, IR. The results indicated that when the stokehole reaction temperature was 1170℃,the decompose temperature of Ferrocene was 150℃,he volatilize temperature of Benzene was 50 ℃, hydric flow rate was 270ml/min, the concentration of thiophene was 1% and the reaction time was 20 minutes, the CNTs with single-walled and multi-walled were prepared. The diameters of the single-walled CNTs are in the range of 0.08~1.15nm, and that of the multi-walled CNTs are in the range of 40~120nm.

结果表明：炉膛反应温度1170℃、二茂铁的分解温度150℃、苯的挥发温度50℃、氢气的流量270ml/min、吩体积分数1.00/100ml苯、反应时间20min时，可以得到碳纳米管，既有单壁管又有多壁管，单壁管的内径分布在0.881.15nm之间，多壁管的直径分布在40120nm之间；纯化后的碳纳米管比表面积增加，管壁光滑，而且产生了大量的含氧官能团，有利于在水溶液中的分散。

Samples of bacterium taken from Huguangyan Maar Lake are isolated and purified using spread plate method and streak plate technique. The partial characteristics is analyzed morphologically and physiologically.

采用涂布法、划线分离法以及形态学和生理生化特性分析，对湛江湖光岩微生物进行了分离纯化及部分性质研究；同时用平板菌落计数法对其异养细菌、真菌和弧菌等的数量进行初步统计。

A low-molecular xylanase produced by Streptomyces sp. Z18 was purified and characterized in this study.

研究了链霉菌Z18中一种低分子质量木聚糖酶的纯化和性质。

Objective: to obtain the high purified and active nerve growth factor from mouse submaxillary glands.

目的：制备具有生物学活性的神经生长因子。

Methods: The NGF purified from mouse submaxillary glands was packed with liposome and prepared into freeze dying agent.

将纯化得到的小鼠颌下腺的NGF用脂质体包裹后分别制成冻干剂型。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。