查询词典 purified

On the condition of the above, the extraction percentage of decolorized and purified tea saponin can be as high as about 89%.

经过上述条件下的脱色和纯化最终得到的茶皂素质量分数可高达约89%。

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

The third aspect studied the stability of the semifinished and purified pigment under different circumstances.

超声法提取枣皮红色素的最佳工艺参数为：超声时间25min，超声功率300W，提取剂的浓度0.9%，料液比1：25，提取级数三级，枣皮红色素的提取率达94.08%。

Purified specific antigen was used in serodiagnosis of tuberculosis and the sensitivity was 89.7%, while specificity was 95.7%.

提纯的特异性抗原成份用于结核病的诊断，其敏感性为89.7%，特异性为95.7%。

The inocula of oocysts were collected from the droppings by purifying with Sheather's sugar flotation method. Two days-old chickens were inoculated by purified inocula of 10,000 oocysts, and vaccinated with one dose of MDV (CVI988 strain) simultaneously.

籍由Sheather's sugar flotation的方法所纯化隐孢子虫的成熟卵囊，经品接种每只10，000卵囊於1日龄之小鸡，并同时皮下接种1剂量的MD疫苗(CV1988株)，於接种后第7天和第12天抽血分离血清，与得自MD病鸡羽毛囊的MD病毒沉降抗原作用，经由琼脂沉？

Initial adherenced cells mostly are round or short fusiform. Division growth of adherenced cells was shown 72 hours after fully changing suspension(.2)Flow cytometry analysis of the adherenced MSCs indicated good homogenicity of passage cells. More than 95% CD34- and CD44+ demonstrated the cells we get were purified MSCs.

2对贴壁生长的MSCs进行流式细胞仪检测，提示传代的细胞均一性较好，表面抗原CD34表达阴性，CD44表达阳性的细胞均在95%以上，说明得到的是比较纯化的MSCs。

And say to him,'Wouldst thou that thou shouldst be purified?

你对他说：'你愿意成为纯洁的人吗？

The purified proteins were separated with 17 cm IPG strips and stained with silver nitrogen, and six protein spots were chosen for mass spectrometry analyses.

随机挖取凝胶上6个蛋白点进行质谱分析，均能获得理想的质谱峰图。

Purified protein samples were separated by 2-DE with 17 cm IPG strips, and the protein spots were detected by fast silver staining. Six protein spots were randomly excised from the silver stained gel for primary analysis by MALDI-TOF MS. Four proteins were identified, and they were 1-aminocyclopropane-1-carboxylate synthase 2, ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit, F11O4.2 and Rubisco small subunit.

根据预实验结果，选用17 cm IPG预制干胶条对纯化的蛋白样品进行2-DE实验，采用快速银染法进行染色，随机挖取银染胶上6个蛋白点进行MALDI-TOF MS分析和数据库检索，鉴定了3个蛋白，它们是：1-氨基环丙烷-1羧酸合酶2，核酮糖-1，5-二磷酸羧化酶/加氧酶大亚基和F11O4.2。

Waste water which had been purified by ion-exchange resins was recovered for chemicals in silvering solutions.

废水经离子交换树脂净化后回收使用，节约了镀银液所需的化工原料。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。