查询词典 purified

In this paper, the application of purified endo-polygalacturonase on elucidating of the chemical structure of pectin was discussed.

探讨了果胶内切酶在果胶化学结构解析上的实际应用。

In addition, the purified IgY was digested with pepsin and the fragment with specific antigen binding was produced.

另外，IgY经胃蛋白酶（pH4.0，37℃）酶解后，得到片断抗体Fab。

The catalytic domain of dipeptidyⅠpeptidase of FAPα is expressed, purified and renatured successfully, establishing a foundation of further research of FAPα.

成功表达、纯化和复性了FAPα二肽酶催化核心区域FAPαc，为深入开展FAPα的研究奠定基础。

Purified by petroleum ether instead of benzene, the results had no obvious difference.

与用苯作为洗脱剂方法测定结果比较，两者无显著性差异。

The organochlorine pesticide were extracted with acetone and petroleum ether, and purified with sulfuric acid.

用丙酮提取样品中残留的有机氯农药，石油醚萃取，萃取液以浓硫酸净化。

Methods Core b1od mononuclear cells were isolated by Ficoll, Cd34(superscript +) cells were purified from mononuclear cells by MACS and then cultured in a complete medium containing rhIL-4, GM-CSF, rhSCF, rhFlt-3 Ligand, TGF-β1 and TNF-α. After 14d of incubation, the morphology of the cells was observed, the phonotype was examined and the IL-12 level in the medium was measured.

Ficoll分离脐血单个核细胞，免疫微磁珠法分离纯化CD34细胞，以rhIL-4、GM-CSF、rhSCF、rhFlt-3 Ligand、TGF-β1，及TNF-α体外诱导培养14d，进行形态学观察、表型检测及上清液IL-12测定。

E. Coli polynucleotide phosphorylase was isolated and purified with a purification factor of 68 times.

本文的第一部分报导了从大肠杆菌中分离多核苷酸■酸化酶的工作。

E.Coli polynucleotide phosphorylase was isolated and purified with a purification factor of 68 times.

本文的第一部分报导了从大肠桿菌中分离多核苷酸砱酸化酶的工作。

Methods The chemical constituents were isolated and purified by silica gel column chromatography and identified on the basis of their physiochemical and spectral data.

方法利用硅胶色谱分离纯化马银花根的化学成分，根据化合物的理化性质和光谱数据鉴定化合物的结构。

Total RNA was isolated from cultured human pigmental cells and mRNA was reversly transcribed into cDNA. Then PCR was used to amplify the TRP -2 coding region. The PCR product was cloned into pUC19 plasmid and sequenced, then subcloned into vector pGEX-4T-1. The TRP-2 protein was expressed hi E coli of DH5 a as fusion protein with glutathioneS-transferase induced by IPTG. The fusion protein was purified by glutathione resin column.

我们通过RT—PCR的方法扩增TRP—2编码基因，克隆至pUC19载体并测序，再亚克隆至GST融合表达载体pGEX—4T—1，转化大肠杆菌，IPTG诱导表达了GST/TRP—2融合蛋白，并分析鉴定表达蛋白的可溶性，最后第四军医大学硕士学位论文一用谷氨酚胺树脂柱纯化表达的蛋白。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。