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The upward mice were inoculated with 2.0×105 oocysts, which were kept at the same time and with the same methods, in 4 ℃, 2.5 % potassium dichromate. 2 days after infection, mice feces were collected and oocyst were isolated and purified used saturated sucrose flotation.

以上各组每只小鼠感染保存时间和方法均相同的微小隐孢子虫卵囊的剂量均为2.0×105个，感染后第3天开始收集鼠粪，收集的鼠粪均用饱和蔗糖漂浮法分离纯化卵囊。

Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21（DE3）. GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA，经逆转录-聚合酶链式反应扩增DOC-1的CDS序列，再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中，构建融合表达载体pGEX-4T-1-DOC-1，经酶切、测序鉴定后，用该重组质粒转化E.coliBL21，用IPTG诱导表达，Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

Escherichia coli 4-fluorotryptophan-substituted arginyl-tRNA synthetase was biosynthetically prepared and purified from a tryptophan auxotroph, which could overproduce this enzyme.

利用色氨酸营养缺陷型菌株在大肠杆菌精氨酰tRNA合成酶中成功地掺入了4-氟-色氨酸。

At first, the PRV as antigen was purified by overspeed centrifugal method.

试验所用的第一抗体为兔抗伪狂犬病毒鄂A株的IgG。

The main impurity involved in the synthesis of erythromycin oxime was separated and purified, which was identified as the very compound formed in the acidic hydrolysis of erythromycin A, i.

分离和鉴定了在合成红霉素肟的过程中产生的一种主要杂质，通过 IR，UV，MS，1 H NMR和 1 3C NMR确证它和红霉素在酸性水解时生成的红霉素 A 8，9-脱水- 6，9-半缩酮为同一化合物。

For this reason, the improving measures, washing entirely equipment, using the diameter under 0.21μm filter, applying ozonizer, had been made so as to the purified water up to standard.

根据以上原因，提出了生产企业应加强设备清洗，使用小于0.2μm孔径的过滤装置，安装臭氧灭菌设备等改进措施，以便使纯净水卫生达到国标规定。

After the recombinant plasmid pAd-VP linearized with PacI transferred into HEK-293A cell,the recombinant virus was isolated and purified in HEK-293A cells by three times plaque purification.

RT-PCR 检测证明目的基因在 mRNA 水平上可有效表达；应用 O 型口蹄疫病毒标准阳性血清进行间接荧光抗体试验，在 rAd-VP 感染的 HEK-293A 细胞的胞质可见清晰荧光。

Pocine interferon α mature protein genes were amplified by reverse transcription polymerase chain reaction, and the recombinant replication-defective human adenovius serotype 5 plasmid pAd-poIFN-α was constructed. When the recombinant plasmid pAd-poIFN-α was linearized with PacI, and then transferred into HEK-293A cells, the virus plaque was isolated and purified in HEK-293A cells by three of plaque purification passages.

本研究利用RT-PCR方法扩增猪α干扰素成熟蛋白基因后构建了重组腺病毒质粒pAd-poIFN-α，经PacI酶切后转染HEK-293A细胞，3次噬斑纯化后获得了重组腺病毒rAd-poIFN-α。

Acquisition of high-quality chromosome suspension: The chromosome suspension released from homogenization suited for flow karyotypic analysis and sorting after fixed in 2% paraformaldehyde for 20 minutes and purified through 10% and 30% sucrose concentration gradient.

高质量染色体悬液制备结果：同步化的根尖细胞经2%多聚甲醛固定20 min，机械匀浆释放染色体并结合10%及30%蔗糖浓度梯度纯化释放的染色体悬液。

Paraxylene is a petrochemical used to make purified terephthalic acid, a raw material for producing polyester film, packaging resin and fabrics.

二甲苯是一种石化产品，用来制造精对苯二甲酸，原料生产聚酯薄膜，包装树脂和织物。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失