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A single chain protein with an apparent molecular weight of 33 kDa was purified from skin of Bombina maxima by a combination of ion exchange and gel filtration chromatography steps. N-terminal amino acid sequence determination indicated that it shares 70%, 64% and 56% identity with those of annexin Ⅱ from the African claw toad, red jungle fowl and human, respectively.

通过阴离子交换、凝胶过滤和阳离子交换层析，从大蹼铃蟾皮肤中纯化到一个表观分子量为33kDa的单链蛋白。N-末端序列比较分析显示，该蛋白与来自非洲爪蟾、红色原鸡和人膜联蛋白Ⅱ的N-末端序列相同的氨基酸分别占70％、64％和56％。

The knotweed is highly purified to remove 99% of the emodin, a natural substance that can act as a laxative in some sensitive people.

对於某些敏感的人来说，虎杖的作用就像天然的通便剂。

Western blotting results showed that the expressed protein had similar immunogenicity with the natural α-BGT protein purified from the venom of Krait Bungarus spp..

经Western blot分析，在大约8 kDa处出现明显的目的带，与预计蛋白分子量大小一致，说明表达产物与天然α-银环蛇毒素具有相似的免疫原性。

When lactide was purified byethyl acetate for 4 times, it was pure enough to polymerize to be PLA.

以乙酸乙酯作为重结晶溶剂，当重结晶次数达到四次后，其纯度已达到丙交酯聚合的基本要求。

Lactoglobulin was purified from lyophilized whey with Sephacryl S 200 gel filtration after casein removed from skimmed milk by adjusting pH to its isoelectric point.

用调pH至等电点的方法将脱脂牛乳中的酪蛋白除去，乳清用SephacrylS－200凝胶过滤，得到较纯的β－乳球蛋白。

First of all, this study will be multi-walled carbon nanotubes by chemical oxidation process purified so it can be modified to deal with than the purification of purity before the modified 5 wt%, more than 1.89 times the surface area to increase the surface potential decrease of about 10 ~ 20 mV and the surface functional base (-COOH and-OH) to increase 1.45 times, and then the control technology of electroless preparation parameters include: analysis of plating time, plating analysis of temperature, metal ions in solution than the (Fe2+/ Ni2+), bath pH and the added value of dispersion Agent and other research towards Fe-Ni particles to increase the iron content and Fe-Ni particles spread in the MWCNT upper fixed targets,the results found that when the parameters for the preparation of 50 ℃, pH10, do not add dispersant, metal ions than the solution (Fe2 +/ Ni2 +) 7, the highest iron content can be Fe = 40.55 at%(flat iron content 111.17 mg / g) of nanocomposites Fe-Ni/CNT, then for a series of the nature of its analysis, the final evaluation Fe-Ni/CNT nanocomposites were processed on the effectiveness of the application of mixed pollutants, the results showed that in 120 minutes at the same time when Adsorption of heavy metal ions lead nitrate 10 ppm, selenium heavy metal ion degradation of 1 ppm and orange azo dye AO7 50 ppm standard of effluents, and after a total Fe-Ni nano-particles to SEM / EDS analysis of more than 50% still remaining.

本研究首先将多壁奈米碳管以化学氧化法做纯化改质处理能使其纯度较纯化改质前提高5 wt％、比表面积提高1.89倍、表面电位下降约10~20 mV以及表面官能基提高1.45倍，再控制无电镀技术之制备参数包括：析镀时间、析镀温度，镀液中金属离子比(Fe2+/Ni2+)、镀液pH值及是否添加分散剂等，研究朝提高Fe-Ni粒子的含铁量与Fe-Ni粒子散布固定在MWCNT上等目标进行，研究结果发现当制备参数为50 ℃、pH10、不添加分散剂、镀液金属离子比(Fe2+/Ni2+)7时，可得到最高铁含量Fe = 40.55 at％(单位铁含量111.17 mg/g)之奈米复合材料Fe-Ni/CNT，接著对其作一系列性质分析，最后评估奈米复合材料Fe-Ni/CNT对共处理混合污染物之应用效益，结果显示其在120分钟时能够同时吸附重金属硝酸铅离子10 ppm、重金属硒酸根离子1 ppm及降解偶氮橘色染料AO7 50 ppm达放流水标准，且共处理后奈米Fe-Ni粒子以SEM/EDS分析仍剩余50 %以上。

In order to provide basis for prevention and cure of this disease, leaf blight pathogens were separated and purified, its biological characteristics were analyzed, and factors such as temperature, acidity, light and medium which influence its growth were studied.

为给此病害的防治提供依据，对该病菌的生物学特性进行了分析，分离纯化了油茶叶枯病原菌，并对影响其生长的温度、酸碱度、光照和培养基进行了试验研究。

In this study,PGCs were isolated from White Leghorn chickenembryos,purified and cultured in vitro.

本研究以白来航鸡为材料，对胚胎期的PGCs进行分离、纯化、培养。

Methods The constituents in the ethanol extraction of the aerial parts of Lespedeza virgate were isolated and purified using column chromatography, and the isolated compounds were identified according to their physicochemical and spectral data.

对细梗胡枝子乙醇提取液的正丁醇部分进行反复分离纯化，根据光谱数据和理化性质确定化合物结构。

The vaccine contains two main protective antigens, leucocytosis promoting factor and filamentous haemagglutinin that are purified from culture supernatant of the Chinese Strain G.

采用百日咳杆菌C.S中国株经培养、盐析、浸提、组分分离以及脱毒等工艺，制备出含有LPF和FHA两种主要免疫原的无细胞百日咳菌苗。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。