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During the isolation of metabolites, we also purified two phase II endogenous metabolites, indoxyl sulfate (IS, 6) and indole-3-acetylglycine (IAA-Gly, 7). from rats' urine.

在分离代谢产物的过程中，我们从大鼠的尿中分离得到2个大鼠内源性Ⅱ相代谢物indoxyl sulfate(IS，6)和indole-3-acetylglycine(IAA-Gly，7)。

The P1 recombinant protein extracted and purified with B-PER GST Fusion Protein purification Kit was used as an antigen, and thallus component of full Mp was used as an antigen control to establish indirect ELISA experimental method to test and determine IgG antibody of serum samples from 40 normal blood donors and 51 clinical serum of partly certain and partly uncertain Mp infector.

以GST融合蛋白层析柱提取、纯化Mp P1重组蛋白做抗原，以全肺炎支原体菌体成分做抗原对照，建立间接ELISA实验方法，检测40份正常献血者血清标本和51份疑似MP感染临床血清标本的IgG抗体。

The purified substance may be emptied into solvent as suitable proportion to dissolve selectively for gold composition in order to achieve infuser liquid of gold-bearing.

通过预处理后的净化物料，由于干扰素已告排除，其中金质成分所占尚存物料总量的比例相应提高，可根据净化物料情况，以适当比例注入溶剂，对金质成分作选择性溶解，以获取含液态金的浸出液。

A purified resin prepared from the flowering tops of the female cannabis plant and smoked or chewed as a narcotic or an intoxicant.

印度大麻麻醉剂由雌性大麻的开花顶端提炼出来的纯丁香，作为一种麻醉剂或毒品点燃或咀嚼

PAO polyclonal antibody was prepared by using recombinant human PAO protein which was purified by polyacrylamide gel electrophoresis as antigen to intradermally inoculate and immunize rabbit.

用制备性聚丙烯酰胺凝胶电泳分离纯化的重组人PAO蛋白，皮内接种日本大耳白兔以制备多克隆抗体。

IPTG induces the expression protein of GST-pUL49j and the induced product was purified electroeluting.The PAGE mortar contained GST-pUL49j was used as antigen, injected intradermally to immunize male New Zealand white rabbit to get polyclonal antiserum.

以含蛋白GST-pUL49j的PAGE胶泥做为抗原，皮内多点免疫注射雄性新西兰兔制备多克隆抗血清，ELISA测定多克隆抗血清效价，Western blot检测多克隆抗血清的特异性。

The optimum temperature and pH for inulin hydrolysis with the immobilized partially purified inulinase were 60℃ and 5.0,respectively.

以菊糖为底物，固定化酶反应的最适温度为60℃，最适反应pH为5.0。

Results show that 4-oxo-β-ionone is the major product with yield of 53.5% under the conditions as follows: reaction temperature 45℃, reaction time 24 h, the molar ratio of n: n(NaClO3): n 20: 100: 3, and pH value of solution in 1-3; on the other hand, 5,6-epoxy-β-ionone is major product with yield of 25.4% when reaction temperature is 40℃, reaction time is 24 h, the molar ratio of n: n(NaClO3): n is 20: 120: 5, and pH values of solution is 3. The excessive β-ionone can be recovered by vacuum distillation for reusing again. 4-oxo-β-ionone and 5,6-epoxy-3-ionone were purified by recrystallization and silica gel column chromatograph respectively with the purity over 98%.

研究结果表明：氯酸钠氧化β-紫罗兰酮的反应主要生成4-氧代－β-紫罗兰酮和5，6-环氧－β-紫罗兰酮，经重结晶和硅胶柱层析分离，纯度高于98%；4-氧代－β-紫罗兰酮的有利合成条件是反应温度为45℃，反应时间为24h，溶液的pH值为1~3，反应物配比n：n(NaClO3)：n为20：100：3，最佳收率为53.5%；5，6-环氧－β-紫罗兰酮有利的合成条件是反应温度为40℃，反应时间为24h，溶液的pH值为3，反应物配比n：n(NaClO3)：n为20：120：5，其收率为25.4%；未反应的β-紫罗兰酮经减压蒸馏回收后可重复使用。

The antibiotic substance was isolated and purified primary using the methods of solvent extraction and Thin Layer Chromatography. The results showed that the antibiotic substance contained four components, and the component Ⅰ and Ⅱ had bioactivity against Cmm.

通过乙酸乙酯抽提、旋转蒸发制备的活性物质粗提液经薄层层析分析后，共分离出四个组份，其中仅组份Ⅰ与组份Ⅱ对番茄溃疡病菌表现抑制作用；紫外及可见光区的吸收峰分别为：253 nm、424 nm和443 nm。

Since isolated and purified from Jack bean in 1919, concanavalin A has been used widely especially as an polyclonal mitogen. ConA can stimulate T lymphocytes blast, induce protein-tyrosine phosphorylation in human neutrophils and increase resistance of mice to peritoneal infection by Serratia marcescens.

ConA从1919年被分离纯化以来得到了广泛的应用，是一种有效的多克隆有丝分裂原，可刺激T淋巴细胞增殖及中性粒细胞酪氨酸磷酸化，增强小鼠对白色念珠菌的吞噬功能、对粘质沙雷菌的抵抗力。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。