查询词典 purified

The invention provides compositions and methods, for reducing one or more symptoms of an autoimmune disease, inflammatory disease and/or transplant rejection, by the administration to a subject in need thereof a pharmaceutically effective amount purified compound of any one of Formulae A-E.

本发明是相关于药物组成物及相关方法，确切地说，是相关于通过对病体的不同需要按配方A-E中的一种对病体给药以一定量纯化的组成物，来减弱一种或多种炎性疾病，自体免疫性疾病，和移植排斥引发的一种或多种症状。

He says of them that, during the generation, they were filled and purified by the Holy Ghost, and freed from sexual concupiscence.

他说，他们说，在一代人，他们被填补和纯化的圣灵，并摆脱性concupiscence 。

Immunoglobulinof ToMV antiserum was purified and conjugated with horseradish peroxidase by sodium periodate. A DAS-ELISA method for ToMV detection was developed using the conjugant.

从制备的番茄花叶病毒(ToMV-S1)抗血清中提纯IgG，并用过碘酸钠法进行辣根过氧化物酶标记，建立了检测ToMV的DAS-ELISA系统。

Specific primers was designed based on conserver sequence of the other animals,firstly,total DNA was extracted from blood of Mongolian sheep and the BMP15 DNA was amplified by PCR,The purified-PCR.product was cloned into PMD18-T vector.

根据其他物种BMP15基因的保守序列设计特异性引物，扩增蒙古羊的全长DNA序列。从蒙古羊的血液中提取总DNA，采用PCR技术扩增出蒙古羊BMP15DNA序列。

Our new Sam's Choice Purified Drinking Water bottle will be the lightest possible gram weight for 20 ounces," says Cott's Ross."

我们的新山姆的选择饮用纯净水每瓶将是最轻的克重量为20盎司，说："科特的罗斯。"

It is extracted mainly from bauxite by electrolysis of a molten mixture of purified bauxite and cryolite.

它主要是由萃取铁铝氧化物得到，而铁铝氧化物是电解含有熔溶之纯铁铝氧化物和冰晶石的混合物而得。

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

By cur common efforts, we will make a clean and purified environment for life and work.

通过共同努力，我们将创造一个清洁、美丽的生活、工作环境。

Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.

首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I，并成功地在原核细胞E.coli进行了重组表达，筛选出稳定表达的工程菌株DH5α，摸索了稳定表达的条件，摸索了包涵体的洗涤、变性和复性条件，摸索出了复性蛋白Src I的纯化工艺，纯化过程简单，经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化，就可获得了高纯度的重组蛋白样品(纯度达99％以上)，该纯化方法适合重组蛋白的大量纯化，为Src I的性质、结构和功能研究奠定了良好的基础。

High-quality stainless steel without toxic substances is used for the pipelines of purified water. There is not dead angle or blind tube in the design and installation. The performance of the equipments and the quality of water produced are consistent with process water requirements.

纯化水所用的管道采用优质不锈钢、无毒、耐腐蚀的材质，设计和安装无死角、肓管，设备运行和产出水质符合工艺用水要求。

John Robbins lives with his family in Santa Cruz, California.

约翰罗宾和家人居住在加利福尼亚州的圣克鲁斯县。

The international background is mainly the economic ties among different nations tented to be widen and deepen at the peace and development times.

国际背景主要是和平与发展时代条件下的世界各国经济贸易联系的日益加深。