查询词典 purified

However, commercially available CPB purified from porcine pancreas was very expensive, and was not totally free of other proteases, such as trypsin and chymotrypsin.

到目前为止，商业途径获得的羧肽酶B 主要从猪胰脏中提取，使得产品价格昂贵，同时也不可避免地混杂有其它蛋白酶，如胰蛋白酶，胰凝乳蛋白酶。

Our laboratory also found a gene like Drosophila intersex in the testis of Cyprinus carpio. According to the significant function played by Drosophila ix in sex-determination, we are interested in the function of this gene in carp. To study the functions of cIX further, I cloned the full-length cDNA of cIX. The recombinant protein is produced and purified to serve as the antigen to induce antibody.

本实验室同样也在鲤鱼的睪丸中发现了类似Drosophila intersex的基因，鉴於intersex在果蝇中所扮演性别决定的重要功能，对於此基因在鲤鱼中所担任的功能而感到兴趣，为了进一步探讨cIX的功能，本论文完成cIX全长cDNA，设计并建构cIX的重组DNA，再利用纯化之重组蛋白诱导抗体。

Results The purified coagulase, with a relative molecular mass of about 350000, reached a purify of more than 97% and contained 17 kinds of amino acids.

用双向免疫扩散试验和T淋巴细胞增殖试验检测其生物学特性。

The present experiment was conducted to determine the optimum dietary zinc requirement of juvenile cobia with zinc methionine as zinc sources, by using a purified diet based on casein-gelatin as the protein sources.

利用酪蛋白和明胶为蛋白源的精制饲料，以有机态的蛋氨酸锌做为锌源，来研究军曹鱼幼鱼对锌的最适需要量。

The basal diet was supplemented with 0, 20, 40, 80, 160 and 320 mg zinc kg(superscript -1) respectively and juvenile cobia(initial weight 7.15±0.85 g) were fed six purified diet containing graded levels of zinc(13.2, 36.6, 52.5, 92.3, 171.2 and 328.2 mg/kg), respectively.

分别向基础词料中添加锌0， 20， 40， 80， 160， 320 mg/kg，制成6种含有不同锌水平的精制饲料(13.2， 36.6， 52.5， 92.3， 171.2， 328.2 mg/kg)，饲养鱼初始体重为(7.15±0.85)g，实验持续8周。

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

It is isolated and purified from a kind of Chinese tropical plants: Coleus forskohlii After many years' research, we found that FSK88 had broad-spectrum effects of anti-cancer and only slightly toxic effects.

研究表明它具有诱导多种肿瘤细胞向正常细胞逆转分化或调亡的作用，而且毒副作用极微。本文以人胃癌细胞BGC-823为体外培养实验模型。

Coli. The expressed protein was purified and used to generate antibodies in rabbits.

在HaSNPV感染的HzAM1细胞中研究了这个基因的转录和表达特征。

Efficient air inlet may be used as terminal high efficient filter equipment to fix in the ceiling of the purified room ,which is characterized by low cost, good seal , common use and easy repair .

高效送风口可作为终端高效过滤装置直接安装在洁净室顶棚等处，具有投资少、密封性能可靠、通用性强、安装方便、维护简单等特点。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失