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Actomyosin were purified from human heart, and further isolated into CMLC with urea degradation and ammonium sulfate precipitation, CMLCⅠ and CMLCⅡ were purified later from this CMLC by the improved PAGE method, both CMLCⅠ and CMLCⅡ were without any protein contamination.

从人心肌提取肌动球蛋白，用尿素降解法和硫酸铵沉淀法从肌动球蛋白中分离、纯化CMLC，应用改进的聚丙烯酰胺凝胶电泳法从总CMLC中分离CMLCⅠ和CMLC Ⅱ，二者均不含有任何杂蛋白。

Glycolate oxidase (EC 1. 1. 3. 1, GO) was purified by 137. 4 fold from leaves of Brassica campestris L. ssp. chinensis var. utilis and the specific activity attained 130.9 U/mg. protein. Chromatography of purified GO through Sephadex G-200 showed two symmetric peaks of both protein and activity, which overlapped fully with each other. Further with electrophoresis on agrose plate, a protein spot and an activity spot were detected in electrophoretogram after respective staining, and the spot of protein covered that of activity, which indicated that the purified enzyme was of a high quality.

从菜心叶片中纯化了乙醇酸氧化酶，比活性达130.9 U／mg，纯化倍数为137.4倍；纯化酶经Sephadex G-200凝胶过滤显现单一而对称的蛋白吸收峰和活性峰，且两者完全重叠；进一步经pH 8.3缓冲系统的琼脂糖平板电泳、染色，各在负极端出现一个活性斑点和蛋白染色斑点，且两者的位置完全对应；表明纯化的菜心叶片乙醇酸氧化酶已达到较高的纯度。

After six rounds of selection in medium containing mycophenolic acid, xanthine and hypoxanthine, we obtained purified recombinant viruses.

提取purified-rHVT感染细胞总DNA电转化大肠杆菌DH10B感受态细胞，在氯霉素抗性平板上筛选阳性克隆，并用酶切和PCR方法对其进行鉴定。

The method comprises steps as follows:(1) Chinese herbal medicines are taken and added with purified water for soaking, with the material-to-water ratio of 1:10;(2) the Chinese herbal medicines with the purified water are placed in a microwave heating system; the power of the microwave and the radiation extraction time are controlled;(3) leaching liquor is poured out and put into a centrifuge for centrifugation to obtain the Chinese herbal medicine water extract;(4) polyethersulfone ultrafiltration film is arranged in an ultrafilter; the Chinese herbal medicine water extract is poured into the ultrafilter and placed in a normal pressure type microwave heating system; and (5) the power of the microwave and the radiation extraction time are controlled and the pressure of nitrogen is regulated so as to obtain the purified Chinese herbal medicine water extract.

本发明公开了一种利用微波辐射改善中草药水提液膜超滤过程的方法，该方法由以下步骤完成：(1)取中草药材以1∶10的料液比加入纯净水，浸泡；(2)置于微波加热系统，控制微波功率和辐射提取时间；(3)倾出浸提液；然后用离心机离心得中草药水提液；(4)超滤器内置有聚醚砜超滤膜，将中草药水提液倒入超滤器并置于常压式微波加热系统；(5)控制微波功率和辐射提取时间，调节氮气压力，得到纯化的中草药水提液。

The suppressive rates to tumor in carboplatin group,white realgar group,middle dose purified realgar group, high dose purified realgar group and purified realgar combined with white arsenic group was 34.3%、35.8%、37.0%、33.1%、37.8%respectively.

精制雄黄低、中、高剂量组、卡铂组、白砒组、白砒+雄黄组肿瘤细胞的凋亡率与模型组比较，细胞凋亡率上升，具有显著性差异(P＜0.05)。3。

Iii. Process Water cont'd Type 3 Water - Purified Water (e.g., USP Purified Water), in Europe the EMEA refers to this a Highly Purified Water - Most difficult to control microbial levels, because no preservative chlorine is present in the water -Less expensive to produce than Water for Injection Type 4 Water - The most critical quality level -Commonly used in final formulation for parenteral applications -Must satisfy the Water for Injection specification as laid out in compendia such as the USP iii.

工艺水 第3类型水-纯化水，在欧洲，EMEA称为高纯水。-控制微生物水平最困难，因为水中没有防腐的氯。-与注射用水比较，生产成本较低第4类型水-最高质量要求-通常用于非肠道用药的最后制剂中-必须符合诸如美国药典这类法定文本中描述的注射用水的规格

Two relevant sites of enzymatic digestion were added to the mTNF-α by PCR. The mTNF-α was linked to the 3'end of m/〓 in pGEX4T-1 vector. The prokaryotic expression vector pGEX4T-1m/〓-mTNF-α was constructed successfully. After induction and expression by IPTG, the expression of two kinds of fusion protein is 15% and 12% of total bacteria proteins respectively. The anti-HCC bifunctional antibodies m/〓-mTNF-α were identified by electrophoresis after the inclusion bodies were purified, denature, renature, re-purified, digested by thrombin and further purified.

采用PCR的方法在mTNF-α的两端加上所需要的酶切位点，将之连接在m／〓的3'端，构建原核表达载体pGEX4T-1 m／〓-mTNF-α，通过IPTG的诱导表达之后，两种融合蛋白的表达量分别占细菌总蛋白的15％、12％，表达产物经包涵体的纯化→变性→复性→纯化→凝血酶酶切→进一步纯化后，可以得到纯度为电泳纯的m／〓-mTNF-α抗肝癌双功能抗体。

Water samples were extracted and purified and collected by solid-phase extraction LC-18 column. Soil samples were extracted by ethyl acetate/petroleum ether (2:1, by vol) and purified by sulphonation. Wheat sample was extracted by acetonitrile/water (4:1, by vol) and purified and collected by SPE- Florisil column.

水样直接用LC-18固相萃取小柱分离、净化和富集；采用乙酸乙酯／石油醚（体积比2：1）振荡提取，磺化法净化，测定土壤中氯氟吡氧乙酸残留量；以乙腈／水（体积比4：1）为提取剂，氟罗里硅土固相萃取小柱分离、净化，测定小麦样品中氯氟吡氧乙酸的残留量。

Dorsalis by salting out, ion exchange chromatography, and gel filtration chromatography. By salting out the egg homogenate, SDS-PAGE analysis revealed that the Vns were mainly precipitated in a 50~70% ammonium sulfate solution. The salted-out proteins were further purified by ion exchange chromatography, and the result showed that Vns were mainly eluted with a 0.3~0.5 M sodium chloride gradient. Finally, the elutes of ion exchange chromatography were refined by running them through gel filtration chromatography to obtain purified Vns. SDS-PAGE separated the 48- and 51-kDa purified Vns, and used them as antigens to raise polyclonal antibodies.

利用硫酸铵盐析法初步分离果实蝇卵中的蛋白质，以SDS-PAGE蛋白质电泳分析得知，卵黄蛋白主要在硫酸铵溶液浓度为50~70%时被沉淀出；盐析沉淀所得之卵黄蛋白，再以离子交换层析法进一步纯化显示，卵黄蛋白主要会在0.3~0.5 M氯化钠的浓度范围间被析出来；再将析出液浓缩后，经凝胶过滤层析法做最后的纯化，即获得纯化之卵黄蛋白。

Methods The purified general bacteria in broth culture for46hourswerepreserved in glycerol-physiological saline,the purified streptococcus and Neisseria scraped from the agar culture were preserved in fresh sheep-blood which was added suitable glycerol-physiological saline,the purified fungi were cultivated in half-solid culture;-20℃general bacteria and fungi,-80℃streptococcus and Neisseria.

将普通细菌的菌种纯化后接种于普通肉汤管，4～6h增菌后，加入适量甘油-生理盐水保存液，-20℃冰箱保存；链球菌属和奈瑟氏菌属的菌株纯化后，用接种环取菌置于新鲜脱纤维绵羊血中，加入适量保存液，-80℃低温冰箱保存；真菌菌种纯化后接种于带硅胶塞的半固体培养基，25℃培养24h后，-20℃冰箱保存。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐