查询词典 purification

Eimeria tenella ; purification ; primary chicken kidney cells ; culture

柔嫩艾美球虫；提纯；鸡原代肾细胞；体外培养

After separation and purification of the expression products by FPLC,the electrophoric patterns of the various eluted peaks are not different with the culture products on SDS-PAGE.

产物经SDS-PAGE、免疫印迹试验与FPLC分离等分析，均表明该工程菌的共表达产物为AchR α-亚基结构域(相对分子质量为13×103)及其与GroES的络合物(23×103)。

Optimum purification condition is elute with 60% methanol with a flow rate of 0.5ml/min .

采用SOURCE 5RPC预装柱检测收集到的洗脱液白藜芦醇纯度可达80.34％。

Further purification was performed on a column of Q-sepharose FF, and the 50mM elute contained the active DM650 with a purity of about 95%.

复性后的蛋白用Q-Sepharose FF离子交换层析进行纯化，50mM NaCl洗脱峰中含有目的蛋白，纯度可达95％。

For purification of BS-63 protein, Ni-NTA resin was used to absorb and elute it from denatured inclusion bodies, based on the principle of chelate.

利用〓和6×Histag螯合的原理，使用Ni-NTA树脂对变性溶解的包含体进行吸附、洗脱。

By the partial purification of Arthrobacter nicotinovorus D-97 endocellular enzymes and the results of enzymatic reaction of enzymes of partition collection, it was found that enzymes related to trehalose synthesis were consisted of Trehalose-forming Enzyme and Trehalose-releasing Enzyme . Trehalose-forming Enzyme could convert maltooligosaccharides into non-reducing maltooligosyl trehalose by intramolecular transglycosylation, and Trehalose-releasing Enzyme could hydrolyze α-1, 4 linkage between the trehalose residue and the maltooligosaccharide residue to release trehalose and maltooligosaccharides with two glucose monomer lesser.

将食尼古丁节杆菌D-97胞内酶部分纯化并对各收集组分进行酶反应，发现其海藻糖合成酶系由海藻糖生成酶(Trehalose-forming Enzyme，TFE)和海藻糖释放酶(Trehalose-releasingEnzyme，TRE)组成，前者分子内转糖基将直链麦芽寡糖转化为没有还原性的麦芽寡糖基海藻糖，后者专一切割麦芽寡糖基海藻糖分子上麦芽寡糖基与海藻糖连接的α-1，4糖苷键，从而释放出海藻糖和减少了两个葡萄糖单体的麦芽寡糖。

These have confirmed the mechanism of NFPLtreating and preventing endometritis to be as follows:(1) bacteria-inhibitingaction,directly extinguishing pathogenic organism;(2) uterus-exciting,promoting purification and involution of the uterus;(3) regulating balance ofreproductive endocrine hormone,accelerating resolution of the inflammation.

以上结果证实，新促孕液防治子宫内膜炎的机理主要在于：(1)抑菌作用，直接杀灭子宫内病原微生物；(2)兴奋子宫作用，促进子宫的净化、复原；(3)调节生殖内分泌激素的平衡，加速炎症的消退。

Erythrina trypsin inhibitor is a Kunitz-type trypsin inhibitor, which inhibits both trypsin and tissue-type plasminogen activator. Immobilized ETI is an effective ligand for the affinity purification of these enzymes.

刺桐胰蛋白酶抑制剂是一种Kunitz型胰蛋白酶抑制剂，能够抑制胰蛋白酶及组织型纤溶酶原激活剂的活性，因此ETI可以作为配体与固定相偶联用于亲和层析。

So it has economic signification that extraction and purification of chlorogenic acid from abundant leaves of Eucommia Ulmoides Oliv.

因此，从丰富的杜仲叶资源中提取分离出较高含量的绿原酸提取物，具有重要的意义。

The separation and purification techniques of chlorogenic acid and flavonoids in Chinese medicine Eucommia ulmoide oliver were tested.

试验研究了杜仲绿原酸、总黄酮的分离纯化工艺。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失