查询词典 purification

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

Finally, 4, 4'-diaminostilbene-2, 2'-disulfonic acid with high quality was synthesized from DNS after purification.

研究结果表明，适宜的重结晶温度约为85℃，优化pH值为9~10，以重结晶后所得精制DNS为原料可制备高品质4，4'-二氨基二苯乙烯－2，2'-二磺酸。

The active form of NGF is a dimer in which two monomer is linked by 3 disulphide bonds. This form of dimer makes the purification of NGF difficulty because the protein expressed in E.

在蛋白纯化过程中这种由二硫键形成的空间结构往往是蛋白纯化过程中复性的难点，尤其是在大肠杆菌表达生物蛋白时，所表达的蛋白往往以无活性的单体形式存在于细胞内。

The efforts of different purification steps were checked by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate.

各步分离提纯的效果用SDS-PAGE证实。

The process industrial synthsis of tert-dodecyl mercaptan from dodecylene and hydrogen sulfide with aluminium trichloride as catalyst was introduced, which consists of four units including the preparation of catalyst, purification of raw dodecylene, additive reaction and separation of reaction product.

介绍了以无水三氯化铝络合物为催化剂，十二烯和硫化氢直接加成法生产叔十二烷基硫醇的工业试验工艺。

Factory covers an area of 45,000 square meters, fixed assets 3,000 million, with 100,000 purification workshop 10000 square meters, the main products have the use of disposable infusion set, blood transfusion, and doser, the use of disposable sterile syringes, injection needles, intravenous infusion needles and other products, annual production capacity of super-400000000, is the domestic production of disposable medical equipment for large-scale backbone enterprises.

工厂占地面积45000平方米，固定资产3000余万元，拥有十万级净化车间10000余平方米，主要产品有一次性使用输液器、输血器、加药器、一次性使用无菌注射器、注射针、静脉输液针等系列产品，年生产能力超四亿支，是国内生产一次性医疗器材的大型骨干企业。

And purification of street perpendicular to a section called dyehouse street alley, there are several special flavored barbecue main course grilled ribs, ribs tender, delicious, very biting head, although many are baked barbecue ribs, but this is my home favorite.

与纯化街垂直有条小街叫染房胡同，有几家烧烤特有味，主菜是烤熟筋，筋嫩，味美，很有咬头，虽然很多烧烤都烤筋，但这家的是我最爱吃的。

Freeing itself from the sensuous world by purification, the human soul ascends by successive steps through the various degrees of the metaphysical order, until it unites itself in a confused and unconscious contemplation to the One, and sinks into it: it is the state of ecstasis.

解放自己从感性世界的纯化，人的灵魂ascends历届步骤，通过不同程度的形而上的秩序，直到它本身的团结在一个混乱和昏迷沉思向一，和汇到它：它是国

Experimental results were as follows:①Temperature restricted the purification efficiency of Eichhornia crassipes and its root-zone microbes.

污水净化中，凤眼莲根系与微生物两者互相依赖，互相促进，在污水降解中表现出良好的协同作用[1]。

Eichhornia crassipes and its root-zone microbes coordinated in pollution purification.

研究了凤眼莲净化污水时，根区微生物的动态变化。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失