查询词典 purification

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The best extraction and purification technology of Coprinus comatus polysaccharide were optimized with the orthogonal design.

本文研究了提取温度、时间、次数和固液比等因素对提取得率的影响，用正交试验优化。

In this paper, modern isolation and analysis methods were used to study on the extraction, purification、characteristics and bioactivities of Coprinus comatus polysaccharides, systematically. The research offers some scientific basis for comprehensive utilization of Coprinus comatus.

本论文以毛头鬼伞子实体为原料，利用现代分离分析手段，对毛头鬼伞粗多糖的提取纯化工艺、理化性质、化学结构以及毛头鬼伞多糖的体外免疫活性进行了较系统的研究。

The results show that:the purification efficiency of cyanide and sulphur increases remarkably with the increase of the electrical motive force and current of the corroding couple and the increase of the area of ...

结果表明，氰、硫的去除率随着腐蚀电池的电动势、电流以及电极面积的增大而提高。由此，为选择高效的电极材料，提供了依据。

5 Are targets of judgment, which the OT sees as purification of God's people and world so that his creative and covenantal purposes are fulfilled:"The earth shall be full of the knowledge of the Lord as the waters cover the sea" Isa.

，是指标的判断，其中城市旅游局认为，作为净化上帝的人与世界，使他的创作和covenantal目的是满足：&地球应充满对知识的主作为水域涵盖海&（以赛亚书11时09分）。

AIM: To study the technological parameters of the purification process of total flavonoids of Crataegus leaves with HPD-600 macroreticular resin.

目的：研究HPD-600大孔树脂纯化山楂叶总黄酮的工艺条件及参数。

METHODS: Using orthogonal experiments,the adsorptive characteristics and elutive parameters of the process were studied by taking the elutive effects and purification ratio of total flavonoids of Crataegus leaves.

采用正交实验法，以固形物中总黄酮含量为考察指标，对影响黄酮提取工艺的因素进行了研究。

Keywords HPD-600macroreticular resin;total flavonoids of Crataegus leaves;separation and purification

HPD-600大孔树脂；山楂叶总黄酮；分离纯化；正交实验

HPD-600macroreticular resin ; total flavonoids of Crataegus leaves ; separation and purification

HPD-600大孔树脂；山楂叶总黄酮；分离纯化；正交实验

After the separation and purification, the crystallin were heated at 60℃ for 30 minutes.

与对照组相比较，晶状体蛋白热凝聚值呈浓度依赖性增加（P〈O.001）。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失