查询词典 purification

The optimum condition was determined via orthogonal experiments, and the result shows that the technology of bile extraction and purification adopts 10L chromato bar with 3L bile, gel silica as fixed phase, the mixed chloroform and isopropanol as mobile phase, gradient elution with mobile phase (the mobile phase ratio in extraction of cholalic acid and glycocholic acid is 4:1 , and then becomes 3:2 in extraction of glycocholic acid and glycocholic acid), and eluting velocity of 3～5mL/s .

采用正交试验筛选了柱层析最佳条件，以容积为10L的层析柱进行分离纯化，固定相为硅胶，流动相为氯仿和正丁醇混合溶剂，一次装样量3000mL胆汁，流动相采用梯度洗脱，先按4：1比例分离胆酸和甘氨胆酸，然后按3：2比例分离甘氨胆酸和牛磺胆酸，流动相洗脱速度为3～5mL/s。

OBJECTIVE: After purification and amplification in vitro, the bone marrow mesenchymal stem cells were induced into chondrocytes, and then implanted them at the bottom of nano-hydroxyapatie/chitosan scaffold for combined culture. The aim was to investigate the feasibility of them used in tissue-engineered chondral composite graft.

目的：将体外提纯、扩增的骨髓间充质干细胞诱导成软骨细胞，将其接种于穿&靴&的纳米羟基磷灰石/壳聚糖支架的底部上联合培养，探索其用于组织工程软骨复合体的可行性。

The extraction and purification methods and the chromatographic conditions were studied.

研究了五种磺胺类药物残留的提取、净化和色谱条件。

ResultsThe AB-8 resin was founded to be suitable for separation and purification of chromone glycoside.

大孔树脂吸附分离技术可有效富集中药活性部位，是提高中药制剂水平的一种有效方法［2～4］。

High-speed countercurrent chromatography is reported for the preparative isolation and purification of a chromone from aloe vera. The crude extract was obtained by a series of pretreatment of aloe vera leaves and extracted from decolorizing active carbon with methanol.

以芦荟全叶为原料，经过一系列的预处理手段，从脱色剂活性炭中获得芦荟色酮粗提物，再经过溶剂分配和富集后采用高速逆流色谱对其中的色酮成分进行分离纯化。

MethodsThe resin suitable for separation of chromone glycoside was determined by static absorption experiment on seven different kinds of resins, and further more, the absorption and elution of chromone glycoside on the selected resin were studied by fixed bed absorption experiment. ResultsThe AB-8 resin was founded to be suitable for separation and purification of chromone glycoside.

方法采用静态吸附实验考察D101，AB8，S8，X5等7种大孔吸附树脂对升麻苷和5O甲基维斯阿米醇苷两种色原酮苷的吸附效果，筛选出吸附性能较好的树脂；对所筛选出的树脂，进一步采用固定床吸附法分离玉屏风提取物中色原酮苷，探讨主要参数对色原酮苷固定床穿透曲线及洗脱曲线的影响。

A polyclonal antibody against vitellin was prepared in male rabbits and the specific antibody against vitellin was made through absorbtion by male worm homogenate. After purification, HRP was used to mark antibody, then ELISA was set up to detect vitellin content in the Chrysopa septempunctata fat-body, hemolymph and ovary.

提取了大草蛉的卵黄蛋白，免疫家兔，并将所得抗血清经雄虫匀浆液吸收，获得抗卵黄蛋白的特异抗体，经纯化后，用辣根过氧化物酶标记抗体，建立了ELISA法测定大草蛉脂肪体、血淋巴和卵巢中卵黄蛋白含量的方法。

METHODS:(1) Ethanol extraction and ethyl acetate purification were used to extract isofiavones from Cicer arietium L.. Orthogonal design was used to optimize the better Ethanol extraction conditions.

(1)以新疆地产鹰嘴豆为原料，经脱脂，乙醇提取，乙酸乙酯萃取，得到总异黄酮，采用正交试验优选出最佳工艺。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。