查询词典 purification

To discover the method of enrichment and purification of total terpenoid components from Alisma Orientalis with macroporous resin.

探讨用大孔树脂富集纯化泽泻总萜醇类成分的方法。

The results showed that the resin AB-8 is the best in character of enrichment and purification of total terpenoid components from Alisma Orientalis. The optimal parameters were radius: height 1:10, the ratio of condensing 1:1, the speed of loading and eluting 10ml/min, the amount ratio of raw herb and resin 4.6:1, 70% alcohol as the elution solvent, the elution volume 4BV.

结果：以AB-8型树脂的富集纯化性能最佳，最优组合：半径、柱高比为1：10；药液浓缩比为1：1；药液上样与洗脱速度为10ml/min；药材与树脂的用量比为4.6：1；洗脱溶媒为70%乙醇；洗脱液量为4倍床体积。

Methods Viruses were passed in embryonated hen eggs, and virion RNA was extracted from allantoic fluid and reverse transcribed to synthesize cDNA. cDNA was amplified by PCR and the PCR product was purified with a purification kit.

方法病毒在鸡胚中传代，从收获的尿囊液中提取病毒粒RNA，通过逆转录合成cDNA.cDNA通过PCR进行扩增，接着PCR产物用纯化试剂盒进行纯化。

More information about their structure and function relationship will be meaningful in understanding the molecular mechanism of ion pump and conformation alteration during ion transport and sensory transduction. In this paper, we report screening strains from samples collected from Ebi lake and Altun moutain Aqik lake, Xinjiang Province, and got 60 clonies from them. We choose 8 of them for further investigation and confirmed that they all belong to the Halobacteriaceae genus and through comparasion of several minute diffrerence medium we had the optimal medium for their growth.. Through this procedure we had established a set of methods for screening clonies from wild environment.and isolation and purification of these archaerhodopsin. Furthermore we used PCR method to identify their DNA sequence.

我们从新疆艾比湖和海拔4250米的阿尔金山阿其克库勒湖中采集到水样、土样和冰样，从中分离出60个单菌落，挑选了8个单菌落进行活化和扩大培养，确证了它们属于嗜盐菌属；确定了它们的最适生长条件；建立了一套完整的从野生环境样品中筛选嗜盐菌菌株的完整方法；摸索出了提取它们中的古紫质蛋白的方法；进行了初步PCR鉴定，为后续研究奠定了良好基础。

Objective: To investigate the antigenic components of Amaranthus spinosus pollen, and to lay theoretical base for the purification of Amaranthus spinosus pollen allergens and the standardization of allergenic vaccine.

目的：分析刺苋花粉的抗原成分以及刺苋花粉特异性变应原组分，为刺苋花粉变应原的纯化和标准化变应原疫苗的研制提供理论依据。

Purpose:In this paper, the source of abundant, inexpensive Amorphophallus konjac as raw material, Purification oligo-glucomannan research methods and conditions were studied.

目的：以来源丰富、价格低廉的魔芋为原料，研究葡甘露寡糖的分离纯化方法及条件。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

The method takes microcrystalline cellulose as affinity column chromatographic medium of Beta-glucosaccharase; under low temperature and anacid condition, the sample of Beta-glucosaccharase is loaded and specifically adsorbed to the microcrystalline column, and then the concentration of NaCl is increased to elute the enzyme, so as to achieve high-efficient and economic separation and purification.

本方法以微晶纤维素作为β-葡萄糖苷酶的亲和柱层析介质，在低温和微酸性条件下，将β-葡萄糖苷酶上样并特异性吸附于微晶纤维素柱，然后再通过增加NaCl浓度的方法将该酶洗脱，从而达到其高效而经济分离纯化的目的。

Results showed that benthic mollusks had some purification effect on CODcr,NH3-N,TP and chl-a of eutrophic river water ,and the effect of Anodonta woodiana was better than that of Bellamya aeruginosa.

文章研究了底栖软体动物富营养化河水中COD、NH3-N、TP、叶绿素a的净化效果。

The thesis is concerned with the fermentation of yeast Candida arborea AS2.566, separation and purification of L-tryptophan and synthesis of 15N labeled anthranilic acid.

本文以发酵法生产L-色氨酸为背景，重点研究了由假丝酵母AS2.566获得L-色氨酸的发酵条件、色氨酸的分离纯化工艺以及邻氨基苯甲酸的合成过程。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失