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Methods: The residual lymphoblast percentage of bone marrow was evaluated on day 14 during a four-drug induction in 21 children with ALL. Telomerase activity of bone marrow cell was determined by telomere repeat amplification protocol -silver stain technique at differential clinical stages.

以多药联合化疗方案进行诱导缓解和缓解后治疗,以诱导治疗第14天的骨髓原始淋巴细胞比例作为治疗反应敏感性指标,以端粒重复序列扩增-银染色技术检测端粒酶活性作为微小残留病白血病指标。

In order to detect the expression of N5, N10-methylene-tetra hydrofolate reductase gene in Epstein-Barr virus transformed human B lymphoblast, as well as the cDNA sequence, human peripheral blood was transformed by EBV to build B lymphoblast cell line. Total RNA was isolated from the cell culture. Variant segments of MTHFR cDNA were amplified using RT-PCR protocol and analysed by polyacrylamide gel electrophoresis.

为检测用EB病毒转化建立的永生性人B淋巴母细胞株中N5,N10-亚甲基四氢叶酸还原酶基因的表达及其cDNA序列,用EB病毒转化人外周血B淋巴细胞,建立永生性细胞株后,从培养细胞中提取总RNA,用RT-PCR法扩增MTHFR基因cDNA的不同片段,进行PAGE电泳分析和cDNA序列测定。

Methods The donor rat spleen was continuously perfused with WZD to select the best protocol. Then the lymphopoiesis was examined with mixed lymphocyte culture and the splenic cells were observed microscopically and immunohistochemically.

以WZD溶液持续灌注移植脾,筛选最佳方案,并观察灌注后混合淋巴细胞培养(mixed lymphocyte culture,MLC)淋巴细胞增殖情况及脾细胞的超微结构,以及灌注后移植脾细胞的免疫组化情况。

To increase average throughput and decrease end-to-end delay of underwater acoustic networks,the original MACAW protocol is improved according to the low quality and high delay characteristics of underwater acoustic channel.

为提高水声通信网络吞吐量并降低端对端时延,针对水声信道低质量和高延迟的特点,对MACAW协议作出了改进。

Therefore, it becomes the main subject for the research of routing protocol in MANET.

因此,对该环境下路由协议的研究成为了移动自组网中研究的主要课题。

This paper mainly discusses the influence of network performance under internal attacks in MANET and security routing protocol.

本文主要研究移动自组网络中内部攻击对网络性能的影响以及安全路由协议。

In order to provide QoS in a MANET, the MAC protocol should be chosen or designed carefully.

为了在随意网路上提供传输服务品质的保证,我们必须要谨慎的选择或设计媒介存取控制协定。

Therefore,designing for routing protocol of MANET faces big challenges.

因此 ,设计MANET的路由协议很富挑战性。

A component that provides functions to a client for manipulation of message data for a particular messaging protocol.

一个为客户端提供的处理特定协议功能的组件。

Aiming at the limitation that IPSec multicast based on GDOI key management protocol is only applied to single-source multicast (one-to-many communication), this thesis proposes a scheme of multi-source multicast (many-to-many communication) of IPSec.

分析了基于GDOI组播密钥管理协议的IPSec安全多播目前只适用于单源多播的应用局限性,基于对IPSec协议及多播安全通信的研究,提出了一种IPSec多源多播的解决方案。

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