查询词典 proteins

Methods The total proteins of untreated and INH-treated mc^2 155 cells were separated by ampholyte pH gradient-based two-dimensional gel electrophoresis (2-DE). Tire differential expression proteins were analyzed by using image analysis software. Results Proteins of control group arid INH-treated group contained 159 and 221 protein spots, respectively.

观察不同浓度的异烟肼处理前后耻垢分枝杆菌生长曲线，确定药物处理SM细胞的最佳时间和浓度，并提取在最佳处理时间和浓度条件下的SM细胞总蛋白，采用载体两性电解质pH梯度双向凝胶电泳技术分离异烟肼处理前后耻垢分枝杆菌mc^2 155细胞的总蛋白质，用PDuest 7.3.1图像分析软件比较分析，以识别差异表达的蛋白质。

The yolk proteins were collected through the anatomization of the maiden eggs and the eggs in different embryo development stages. The yolk proteins were separated by two-dimensional electrophoresis and followed the image analysis. Over 203 protein spots were found to exist in the yolk protein of silkworm eggs. Some different patterns of yolk protein spots were found in silkworm of various varieties. While, little variation of yolk proteins was detected during different development stages of any silkworm variety.

采用直接解剖蚕卵获取卵黄蛋白，结合蛋白质双向电泳和图像分析，对家蚕处女蛾卵及不同胚胎发育阶段蚕卵的卵黄蛋白质进行了研究，发现家蚕卵黄蛋白有 203 个蛋白质斑点；不同家蚕品种之间卵黄蛋白的组成存在一些差异；同一品种不同发育时期胚胎卵黄蛋白的组成基本保持不变。

The results show that all the hmgr genes from pigs, cattle and chickens are astable enzyme proteins with the same gene sequence characteristics and encoding protein and physical and chemical properties; all the amino acid sequences have a high degree of homology, containing two conservative HMG-CoA binding motifs and two NADPH binding motifs; all the amino acid sequences, containing signal peptide, are secreted proteins; they are all hydrophobic transmembrane proteins with five transmembrane domains; random coil and α-helix are the main structural elements in the HMGR polypeptide chain; all the predictions of three-dimensional structure are V- shaped; there are more closer evolution relationship between the HMGR gene from pigs and cattle.

结果表明：猪、牛和鸡HMGR基因序列特点及编码蛋白质理化性质基本相同，均属不稳定类酶蛋白；保守结构域氨基酸序列具有高度的同源性，均含有保守的2个β-羟基-β-甲基戊二酸单酰辅酶A结合基序和2个还原性烟酰胺腺嘌呤二核苷酸结合基序；均有信号肽，属分泌型蛋白质；属于跨膜的疏水性蛋白，均有5个跨膜结构域；无规则卷曲和α-螺旋是HMGR多肽链中的主要结构元件；三维结构预测都呈"V"字形；猪和牛HMGR基因的进化关系最近。

Results①Make clear of the general principal during the morphologicaldevelopment of the C57BL/6 mouse inner ear and reconstructed the 3D modelof E13 inner ear;②The mature time of many histological structuresassociated closely with the hearing setout of C57BL/6 mouse were madeclear as basilar membrane in D10, scala vestibular and tympanicD4, Corti\'s tunnel D12, inner sulcus D10, tectorial membrane D10, haircells D12, spiral ganglion neurons D12 and neurofilament in cochlear axisD12-D14.③Outlined the time table and the patterns of the expressionsof those proteins associated with the inner ear development which includedproneural committed proteins, neuronal differential proteins,neurotrophic factors and neuron special proteins.

结果①HE常规染色大体观上了解了C57BL／6小鼠内耳形态发育E8-Da期间的一般规律并且获得了E13的三维立体图像；②获得了各种听功能密切相关的结构的成熟时间点，基底膜(D10)、鼓阶前庭阶(D4)、螺旋器隧道(D12)、内螺旋沟(D10)、盖膜(D10)、毛细胞(D12)、螺旋神经元(D12)和蜗轴纤维(D12-D14)；③完成了前神经决定蛋白、神经分化蛋白、神经营养因子和神经元特异性蛋白等内耳发育相关的蛋白的表达时限表并详细观察了其在内耳各个部位表达随发育变化的特点：④Calbindin在E16以后的螺旋神经元没有表达，但是在scarpa神经元持续表达。

A total of 78 differential proteins were identified, among these proteins, 42 proteins are over-expressed in gastric adenocarcinoma tissues and 36 proteins are down-expressed.

共筛选出78个差异表达蛋白，其中42个蛋白质在胃癌组织中表达上调，36个蛋白质下调。

The average degradation percentage in sarcoplasmic proteins, myofibrillar proteins and stroma pronteins were ordinally 62.07%, 46.15% and 8.56%, so that substances coming from degradation of sarcoplasmic proteins and myofibrillar proteins notabily effected qualities of jinhua hams.

在主成分分析中，总氮、肌原纤维蛋白、pH值、肌浆蛋白、PeN、浙江大学硕士学位论文NPN和核酸态氮对金华火腿理化特性的解释能力可达81 。38%，反映了金华火腿理化特性变异的绝大部分信息。

The identified proteins are involved in a variety of cellular process including several zinc finger proteins relevant to transcription regulation, such as zinc finger 198, 263, 14, 224, zf6, novel protein similar to transcriptional represser CTCF, and kruppel-like zinc finger protein; two members of the ADAMs (a disintegrin and metalloprotease domain) family; two members of integrin family; several proteins involved in the signal transduction, cell-cycle control, chromatin remodeling and transcription repression; and also some proteins of cell skeleton and some with unknown functions.

鉴定出 的蛋白包括多个与转录调控相关的锌指蛋白，如锌指蛋白198、263、14、224、zf6、转录抑制因子cTcF样蛋白、幻肚pple样锌指蛋白等：两个含金属蛋白酶结构域和整合素结合结构域的家族成员ADAM28和ADAM17；两个整合素家族成员pZ整合素和含十个EGF样结构域的整合素；与细胞信号转导通路有关的蛋白；与细胞周期调控有关的蛋白；与染色体重塑和基因转录抑制有关的蛋白；细胞骨架蛋白以及其他功能未明的蛋白等。

UPP can degrade intraeellular proteins with high effect and selectivity, especially for cell cycle regulated proteins, oncoproteins, tumor suppressor proteins, and denaturalized proteins.

当泛素-蛋白酶体系统对靶蛋白的降解功能失常时，可以引起癌蛋白聚集、抑癌蛋白异常降解、突变细胞凋亡受阻和增殖加速，从而导致肿瘤的发生。

He analysis of the category and function of TCB stress inducible proteins showed that different groups of proteins were induced by 5 mg L^(-1) TCB stress. They are detoxification enzymes (including esterase, aldo/keto reductases, and glutathione S-transferase), cell wall compound metabolism related enzymes (including UDP-glucose protein transglucosylase and GDP-mannose 3,5-epimerase 1), phytohormone metabolism and regulation related enzymes or proteins (including aci-reductone dioxygenase 4, beta-glucosidase, two members of pathogenesis-related proteins from family 10), primary and secondary metabolism regulative enzymes (including translational elongation factor Tu, cytosolic orthophosphate dikinase, triosephosphate isomerases, alanine aminotransferase, and isoflavone reductase).

CB胁迫能诱导根系内不同类型蛋白质的表达，它们是：解毒酶（包括脂酶、醛／酮还原酶、谷胱甘肽－硫转移酶），细胞壁物质代谢相关酶（包括蛋白转葡萄糖基酶、GDP-甘露糖－3，5-异构酶1），激素代谢或调节相关酶（包括酸式－还原酮二加氧酶、β-葡萄糖苷酶、病程相关蛋白家族10中的2个不同蛋白），原初或次生代谢相关酶（包括转录延长因子、细胞质磷酸丙酮酸双激酶、磷酸丙糖异构酶、丙氨酸氨基转移酶和异黄酮还原酶）。

In order to identify the proteins which are responsible for the better fruit set in heat-tolerant variety than in heat-sensitive one, we used two dimensional electrophoresis systems to separate isolated proteins from developing anther and looked for the proteins up-/down-regulated in heat-tolerant variety. In doing so, two selected cultivars, CL5915, the heat-tolerant, and L4783, the heat-sensitive, were grown at the same time in a phytotron at both 30/25℃ and 25/20℃. The proteins isolated from developing anthers of heat-tolerant variety grown in 30/25℃, were subtracted to remove the common genetic background.

因此为了鉴定出在高温逆境下与番茄著果率提升有关之蛋白质体，我们利用亚蔬中心所提供耐热番茄(Solanum lycopersicum， formerly Lycopersicon esculentum)品系CL5915与不耐热品系L4783，藉由控制二品系间生长温度之差异（热逆境30/25℃与一般生长环境25/20℃），在雄蕊发育早期利用蛋白质体学技术分析可溶性蛋白质，比较并扣除共有之遗传背景之蛋白质后，将二品系间具有差异的可溶性蛋白质筛选出来，并进一步利用LC-ESI MS/MS进行蛋白质身份鉴定。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。