查询词典 proteins

We initiated a genetic screen for Drosophila olfactory genes by means of SG18.1-Gal4/UAS system: The result validates our screen as an rapid, effective approach for recovering genes controlling glomerular map patterning; From a misexpression screen of 1,515 P{GS} lines, we identified 23 genes that, when forcibly expressed in the olfactory receptor neurons, disrupted the stereotyped anatomy of the Drosophila antennal lobes; These genes, which have not been shown previously to control olfactory map development, encode novel proteins as well as proteins with known roles in axonal outgrowth and cytoskeletal remodeling.

本文首次利用SG18.1-Gal4／UAS基因筛选系统对果蝇嗅觉基因进行了筛选和鉴定：结果表明，所采用的方法可以快速、有效地分离到果蝇嗅觉相关基因；从1515株果蝇P{GS}品系分离到86株突变体，其中40株有明显表型，从中鉴定了23个基因，这些基因在ORN强迫表达时可以损坏果蝇嗅叶的固有结构；在筛选中发现的一些调节嗅觉图谱发育的基因可以编码新蛋白或编码参与轴突生长和细胞骨架重塑的蛋白；为了便于研究，根据基因的功能和所编码蛋白的特点，我们对分离到的基因进行了分类，即参与轴突引导和突触产生、调节转录、调节细胞骨架和功能未确定或未知的基因；并通过对嗅觉感觉器的检测发现，除了调节转录的基因外，其他基因对触觉器的数量和分布影响不明显。

For their treatment experiment, the researchers relied on a class of proteins, bone morphogenic proteins, that cause neural stem-cell-like clusters to lose their stem cell properties, which in turn stops their ability to diide.

针对它们的治疗试验，研究者依赖一类骨形态发生蛋白。这种蛋白引起神经母细胞样簇失去母细胞的特性，从而终止了它们的分化能力。

Wingless proteins and bone morphogenic proteins appear to play a central role in the ''plus'' variant of inflammatory bone remodeling.

Wingless蛋白和骨形成蛋白在&plus&型炎性骨重塑中似乎起着核心作用。

TGF-β s are multifunctional cytokines with a broad range of biological activities. TGF-β s belong to a family of related proteins, which demonstrate various degrees of amino acid sequence homology; this protein family is called the TGF-β superfamily. Other members of this family include the bone morphogenetic proteins, inhibin, activin and Mullerian substance.

TGF-β类生长因子是一类具有广泛生物学活性和重要临床应用价值的细胞因子，包括多种蛋白因子，它们在氨基酸结构上具有不同程度的同源性，共同构成了TGF-β超家族，包括BMPs、活化素、抑制素和缪勒氏物质等。

Recently, a variety of regulatory proteins including DNA methyltransferases, methyl-CpG binding proteins, histone-modifying enzymes, chromatin remodeling factors, and their multimolecular complexes have been identified.

最近，包括DNA转甲基酶，CpG甲基化结合蛋白质，组蛋白修饰酶，染色质重构因子，和它们的多分子复合物等各种调控蛋白质被识别。

There was no identical proteins in the database we searched, but it shared high sequence homology with PNZIP from Pharbitis nil and two other open reading frames of unknown function from Synechocystis spp and Porphyra purpurea. These together defined a new family of evolutionarily conserved leucine zipper proteins, which might play a role as a transcription factor in the regulation of gene expression.

GenBank数据库搜寻结果显示，AT103未与任何已知功能基因有同源性，但与裂叶牵牛PNZIP、集胞藻的一个ORF、紫菜的一个ORF，构成了一个在进化上非常保守的含有亮氨酸拉链结构域的新家族，极有可能是一新的核基因转录因子。

Working with global clients, Selexis offers contract development and maintenance of high-performance mammalian cell lines used in the expression of pharmaceutically relevant proteins (i.e. membrane receptors, MAbs, plasma proteins, rProteins in general).

与全球客户，提供合同Selexis发展和维护高性能的哺乳动物细胞系中使用的表达pharmaceutically相关的蛋白质（即膜受体，单克隆抗体，血浆蛋白， rProteins一般）。

Western blotting show that benzyl isothiocyanate and phenethyl isothiocyanate treatment gradually decreased the level of anti-apoptotic proteins, but increased the level of pro-apoptotic proteins.

接著，由西方点墨法的结果发现苯甲基异硫氰酸酯和苯乙基异硫氰酸酯会造成人类前列腺癌细胞株DU-145凋亡蛋白的增加和Caspase活性的上升。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Pokeweed antiviral protein, a 29-kD protein isolated from Phytolacca americana, inhibits translation by catalytically removing a specific adenine residue from the large rRNA of the 60s subunit of eukaryotic ribosomes. In addition to its ribosome-inactivating ability, PAP has potent antiviral activity against many plants and animal viruses.

美洲商陆抗病毒蛋白(pokeweed antiviral proteins，PAPs)，是在美洲商陆的不同组织或不同生长阶段生成的一类具功能的单链核糖体失活蛋白(ribosome-inactivating proteins，RIP)，是天然的广谱抗病毒生物活性物资，能抗多种植物和动物病毒。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。