查询词典 proteins

Hspa5 belongs to a member of the Hsp70 family. It plays important roles in facilitating fold and assembly of newly synthesized proteins and their translocation across the endoplasmic reticulum membrane as a molecular chaperone, helping refold stress-denatured soluble proteins and regulating signal transduction pathways by modulating the stability and activity of protein kinases and transcriptional factors.

Hspa5为Hsp70家族的成员，它可以作为分子伴侣促进新合成蛋白质的折叠、组装及其跨过内质网膜的转运；使应激变性的可溶性蛋白质重新折叠；通过调节蛋白激酶或转录因子的活性而控制信号转导途径。

Methods Three different resolvable solutions were adopted to take out the proteins of MOLT-4 cells as the example, and then the proteins were separated by two dimensional polyacrylamide gel electrophoresis.

以MOLT-4细胞为例，采用3种溶解性能不同的裂解液提取细胞中的蛋白质组，并分别进行双向聚丙烯酰胺凝胶电泳。

Both 47- and 56-kDa proteins are considered as the major surface proteins of Ot, which has a potential importance in development of subunit vaccine against scrub typhus.

研究证明，这两种外膜蛋白均存在菌体表面并具有良好的免疫原性，已成为恙虫病东方体亚单位疫苗的重要候选分子。58kDa蛋白是恙虫病东方体的热休克蛋白，属于Hsp60家族。

The thesis consists of six chapters. In the first, the technologies of atomic force microscopy and of the measurement elasticity of biomolecules were introduced. In the second, the validity of VSPFM was confirmed by lift mode atomic force microscopy. In this chapter, the height of DNA was measured by lift mode atomic force microscopy, which demonstrated that the method of height measurement of biomolecules by VSPFM was correct and established the foundation of the method of measurement elasticity of biomolecules by vibrating mode scanning polarization force microscopy. In the third chapter, detailed work has been illustrated on the foundation of the method of measurement elasticity of biomolecules by VSPFM. And the compressive elasticity of DNA was measured. In fourth and fifth chapters, the method was applied in the measurement elasticity of proteins. Two proteins elasticity, fibre-like protein α-synuclein and global protein IgG, were measured by VSPFM, through which the method wound its way to the application of biomolecules. In last chapter, the final part of the thesis was a summary. A conclusion of the thesis and a self-comment on my work as a PhD candidate have been made, and expectation about the further works has been addressed.

本论文共分为六章，第一章，引言部分主要介绍了原子力显微镜技术及生物大分子弹性测量技术；第二章主要是VSPFM方法的正确性论证，介绍抬高模式原理，利用抬高模式原子力显微镜对DNA的高度进行测量，论证振动模式扫描极化力显微镜测量生物大分子的高度的正确性以及准确性，从而为振动模式扫描极化力显微镜测量生物大分子的弹性方法的建立奠定基础；第三章以脱氧核糖核酸为例详细介绍了振动模式极化力显微镜测量生物大分子弹性的方法的建立，对DNA的压弹性进行了初步的测量和分析；第四章和第五章介绍了振动模式扫描极化力显微镜在蛋白质弹性测量中的应用：α-synuclein和IgG分别是纤维状蛋白和球状颗粒蛋白，通过振动模式扫描极化力显微镜测量这两种蛋白质的弹性，摸索振动模式扫描极化力显微镜在蛋白质弹性测量中的应用；第六章对全文进行了总结，在对论文的工作进行归纳和自我评价之后，还对进一步的工作进行了展望。

Mainly using protein - film voltammetric technique, we have achieved direct electrochemical response of lots of proteins such as hemoglobin, myoglobin, cytocrome c, cytocrome b5, horseradish peroxiase, GOD ,Vitreoscillia sp. haemoglobin, etc., at a series of protein membrane modified electrodes, for instance, protein/kieselgubr, protein/polyethylene glycol, protein/polyethyleneimine, protein/phosphatidylcholine, protein/DNA, protein/montmorillonite/polyvinyl alcohol, protein/silver nanoparticle/ cysteamine membrane modified electrode, etc., which are prepared by casting, incorperating or layer by layer self-assembly methods. Electrochemical behavior of these proteins in the films have been studied, and their structural and conformational changes have also been examined with spectrometric techniques.

三年来，在基金委的支持下，我们以蛋白膜伏安法这一新技术作为主要的研究手段，并结合其他生物、化学技术，研究了血红蛋白、肌红蛋白、细胞色素C、细胞色素b5、辣根过氧化物酶、葡萄糖氧化酶以及好氧革兰式阴性菌透明颤菌中的血红素类蛋白等多种蛋白质在以涂布和自组装方法制成的蛋白质/硅藻土膜、蛋白质/聚乙二醇膜、蛋白质/卵磷脂膜、蛋白质/聚乙烯亚胺膜、蛋白质/蒙托石/聚乙烯醇自组装膜以及蛋白质/银胶/半胱胺自组装膜等修饰电极上的直接电化学行为，结合各种光谱技术，对蛋白质在膜中的构象进行表征，探索其电子传递机理。

EST similarity analysis was finished by comparing sequences with BLASTx searches in NCBI. The results showed that they are proteins involved in biological processes such as energy metabolism and RNA molecular level regulation. Some of them are enzymes and structural proteins in the cell. This study provides useful information for future studies on molecular mechanism of filament coloring and for breeding new silkworm variety that spin color cocoons.

通过BLAST比对和对所得表达序列标签的同源分析表明，这些基因主要涉及能量代谢因子、RNA分子水平相关调节因子、酶类、结构蛋白等，由此初步探明了参与黄血基因协同作用的相关基因表达蛋白的种类和数量，可为进一步研究蚕茧着色的分子机制及通过遗传选择培育天然有色茧品种提供基础信息。

"While preious research has demonstrated that arious types of stem cells can turn into cells that express the proteins consistent with smooth muscle, this is the first study that shows that the cells we generated hae the same functional properties as smooth muscle, as well as express the same proteins," said Jeffrey Ross, Ph.D., research associate at the Stem Cell Institute.

"以前的研究已经证实多种不同类型的干细胞可以被转化成表达与平滑肌细胞相同蛋白的细胞，这个实验首次显示，我们得到的细胞由于平滑肌相同的特性，表达一样的蛋白，"干细胞研究所的研究员Jeffrey Ross博士说。

About 20 members of importin β superfamily proteins are responsible for signal-dependent transport of nuclear proteins in mammalian cells.

在哺乳动物细胞有20个蛋白质负责依赖于信号的蛋白质在细胞核质间的穿梭。

The regulation of gene expression is to have relations to the assembly of the DNA binding proteins which bind to DNA to initiate the transcription. Some of these proteins have the ability to promote bending or looping of DNA. The FIS protein regulates gene expression in Escherichia coli by this property of bending DNA. There are many researches to discover the mechanism of FIS protein binds to DNA. However the experiment data only show the supposititious results and figure out the model of FIS-DNA complex structure. This research uses molecular dynamics simulation to compute the detail of the interaction between FIS and DNA. We observed FIS prominently affects the DNA bending by the type of local bending. Analyzing the DNA structure properties, roll and slide, also demonstrates the local bending type and region. The hydrogen bonds between FIS and DNA are analyzed to compare with the experiment data. Finally we compute the binding free energy between FIS and DNA to estimate the result of simulation and compare with the value of λ-repressor-DNA complex.

基因的表现与结合於DNA促使DNA转录作用起始的DNA结合蛋白组装有关，一些这类的蛋白质具有促使DNA扭曲或将DNA形成环状的能力，FIS蛋白质即是藉由扭曲DNA的特性调控大肠杆菌基因的表现，目前已有很多的研究发现FIS与DNA结合的机转，然而这些实验的数据仅能显示推测性的结果，进而利用这些结果推论出FIS与DNA聚合体结构的模型，本研究利用分子动态模拟的方式计算FIS与DNA交互作用的细节，我们观察到FIS主要影响DNA的扭曲方式为DNA区域扭曲的类型，并且分析DNA结构特性roll与slide也指出扭曲方式为区域扭曲与其扭曲的区域，我们也分析FIS与DNA之间的氢键，并且与实验的资料作比较，最后我们计算FIS与DNA之间的结合自由能评估模拟的结果，并且计算出的值和λ-repressor与DNA聚合体结构的实验值作比较。

As high activities in CS reflecting aerobic nature of the tissue, the present dataset suggests that the swim bladder muscles should involve with sound production. Proteomics analyzes identified 14 proteins from the swim bladder muscle, one of them are related to energy production. Two-dimensional gels show some different spots from these three type muscles. These results help us to find some different proteins in these three types of muscles of this fish.

以蛋白质二维电泳分析鱼鳔附属肌肉，共鉴定出14种蛋白质，其中一种与能量相关的蛋白质与体侧肌的蛋白质表现不同，而此蛋白质表现与尾部红肌相似，此结果可望帮助我们找到鱼鳔附属肌肉与其他不同类型肌肉的相异之处。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。