查询词典 protein

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

BST16 was also proved to encode gene of PSII lOkD protein by protein prosite and conserve domain analysis. The protein encoded by BST16 contained a conserve domain PsbR of PSII lOkD protein from 48 to 140 and its carboxy terminal had an ADH_SHORT prosite of Short-chain dehydrogenases/reductases family signature from 94 to 122, which showed the protein encoded by BST16 would be a reductases.

对该基因可读框架编码的蛋白进行功能位点和结构功能域的分析，同样证明了该基因为PSⅡ 10kD蛋白编码基因，其氨基酸摘要序列 48刁位含有一个保守的光合系统＊ 10kD蛋白结构域 PSbR，在蛋白的猿基端有一个保守的短链脱氢酶／还原酶家族特征序列ADHSHORT（Short－chain dehydrogenases／reductases family signature），位于94－122位，说明了该蛋白可能具有还原酶活性。

After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对，结果显示，PPV-SC1 NS1与其他的PPV NS1的同源性较高，仅存在个别的差异，分别是第39位A→G，第153位T→C，第175位A→G，第1117位A→C，第1535位A→C；同源搜索比较表明，PPV-SC1与PPV NS1同源性可达98％、99％，与其他的细小病毒NS1基因也存在很大的保守性；密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性；PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段，用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区，返回的结果并没有得到有显著意义的跨膜区的存在；根据基于motif数据库的结构域预测，PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP／GTP结合位点，该蛋白还存在16个蛋白激酶C磷酸化位点，21个酪蛋白激酶2磷酸化位点，3个cAMP-／cGMP依赖蛋白激酶磷酸化位点，PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域，推测NS1可能与POX_D5有类似的功能。

So we understand to once and actually usually take place of the bad factory house adds plant egg white(soybean egg white etc. corn egg white) in the origin should be the milk powder of animality egg white, this is already the first floor to cheat and commit crime, dishonest at least, but the widespread phenomenon in the in view of the fact industry, and have no obvious side effect to the human body, therefore is connive;But the protein content in the original inferior product very low, unexpectedly meet more unscrupulous plant egg white raw material to provide a company, add three gather cyanotype An, result in as a result currently result;Here our for the time being imagining is the beperhaps havior of the supplier, perhaps is three deer companies have intention to add, the basic reason for add lies in a current Kai surname settle nitrogen protein measurement method can test an always organic nitrogen content, rather than the nitrogen content in the particular protein, therefore, the method blemish was fume by the cupidity the exploitation that the milk powder manufactories of heart speculate, make the false and inferior product deceive to reach to mark, because they know three chlorine cyanotype An toxicities are again very small, disguise a protein content to reach a mark in the examination, but they didn't thought of that the toxicity is pimping three chlorine cyanotype An exactly will bring serious Bi to baby kid to wet system stone calculus, we even can guess, other adults of three deers use a milk powder in must also imply in great quantities similar chemistry product, just the adult will not get instant results of is endanger by body.

那么我们了解一下，实际上经常发生的不良厂家在本应该是动物性蛋白的奶粉里添加植物蛋白，这已经是第一层欺骗和犯罪，至少是不诚实了，但由于是业内的普遍现象，且对人体无明显副作用，因此被默许；而本来劣质产品中蛋白质含量就很低，没想到遇到更缺德的植物蛋白原料提供商，添加三聚氰胺，结果造成目前后果；这里我们姑且想象也许是供应商的行为，也许是三鹿公司有意添加，添加的根本原因在于现行的凯氏定氮蛋白质测定方法只能测试总有机氮含量，而非特定的蛋白质中氮含量，因此，方法缺陷被利欲熏心的奶粉制造商们所投机利用，使伪劣产品蒙混达标，因为他们知道三氯氰胺毒性很小，又能在检测中伪装蛋白质含量达标，但他们就没有想到毒性很小的三氯氰胺却恰恰会对婴幼儿造成严重的泌尿系统结石，我们甚至可以猜测，其它三鹿的成人用奶粉中一定也含有大量类似化学制剂，只是成人不会立竿见影的受到身体危害。

The female salivary gland proteins were influenced by JH Ⅲ. Treatment on d2 after feeding, 10μg dose increased SG protein level and restrained 136kD protein gene expression. Treatment on d0 after engorgement, 10μg, 50μg and 100μg doses significantly increased the d2 SG protein and 100μg dose made 35kD protein gene expression, 200μg treatment made the 136kD and 192kD proteins absent. This is the first found that JHs regulate arthropoda SG activity.

保幼激素对雌蜱唾液腺蛋白及成分有影响，吸血后2天处理，10 μg剂量使唾液腺蛋白含量明显增加，抑制136kD蛋白的基因表达；饱血当天处理，10μg、50μg和100μg显著提高饱血后2天唾液腺蛋白含量，100μg使35kD蛋白表达，200μg使136kD和192kD的蛋白缺矢；饱血当天处理，1μg和10μg剂量均能显著提高饱血后4天雌蜱唾液腺蛋白含量，高剂量（100μg）抑制136kD蛋白的表达，该结果首次报道了保幼激素对节肢动物唾液腺的调控作用。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

Increasing of ionic strength screened the repulsive forces between protein-chitosan, protein-protein at pH 7.4. As a result, the amount of adsorbates was increased. However, higher concentration of supporting electrolyte induced the decrease in BSA adsorption for chitosan chains tended to curling to be conglobation, which resulted in fewer sites to contact with protein.

离子强度的增加，屏蔽了蛋白质与壳聚糖表面、蛋白质-蛋白质分子之间的静电斥力，使得BSA的吸附量增加；而当盐浓度大于0.1 mol/L之后，由于壳聚糖分子链的刚性变小，容易成团，与蛋白质接触面积减小，导致吸附量下降。

Tata and protein powder into the stainless steel basin, with eggbeater whipping to a bubble-like protein was rough and white colors, white sugar into the next and continue whipping until soft peaks form protein was (that is, the cream of the protein does not stand Feng Jian Sag) and rigid foam.

蛋白和塔塔粉纳入不锈钢盆里，用打蛋器搅打至蛋白呈粗泡沫状且颜色发白时，下入白糖，继续搅打至蛋白呈软峰状（即蛋白膏的峰尖挺立不下垂）并硬性发泡。3。

It was found that there were 36 distinct differences in 184 protein spots. In-gel digestion and liquid chromatography mass spectrometry C LC-MS were made to analyze the protein SSP2801, and Mascot software was applied to identify the protein. The results showed the protein was large subunit of Rubisco, and its content in yellow mutant decreased distinctly, only about 26% of the comparison. Maybe it has relation to etiolation.

切耿其中差异最为明显的SSP2801位点蛋白进行胶内酶切、LC-MS分析，并应用Mascot软件检索鉴定，结果表明该蛋白组分为RUBP羧化酶的大亚基，其在突变体中含量明显减少，仅为对照的26％，表明突变体叶色黄化与RUBP羧化酶大亚基含量减少也有关系。

Congregation obtained after chitosan k and a treatments showed more tightness than sediments obtained without chitosan treatment, sediments after chitosan b and c treatments were network in structure. Mixed systems of pectin, protein and polyphenol were applied for studying a simulated system of kiwifruit juice. It was shown that the aggregates of pectin molecules in pectin solution were dispersing tubular globules. Particles in mixed solution of pectin and bovine serum albumin had two states: one was circular in photo, the other contained single particle or aggregates of several particles. Pectin decreased the interaction between protein and polyphenol and strengthened the stability of mixed solution of pectin, protein and polyphenol. Chitosan flocculated mixed solution of pectin, protein and polyphenol, and the floccule was white, having a network structure.

用果胶-蛋白质-丹宁酸混合体系作为猕猴桃原果汁的模拟体系研究结果表明：1、果胶溶液中果胶分子聚集物呈中空球形均匀分散；2、果胶／牛血清白蛋白混合溶液中的球形粒子有两种：粒子中央光线可透过，照片上呈环形；粒子的中央包含有单个或多个球形粒子的聚集体，认为粒子的中央是蛋白质，外层是果胶；3、果胶的存在抑制或减弱了蛋白质-丹宁酸之间的相互作用，果胶起到防止蛋白质-多酚产生沉淀的作用，增强了溶液的稳定性；4、壳聚糖加入果胶-蛋白质-丹宁酸混合溶液中时，可以絮凝果胶-蛋白质-丹宁酸混合溶液，溶液中出现白色的网状絮凝物。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作： 1 回顾了直线电机发展历史，电机的驱动技术演变，特别是针对直线电机的驱动，简要介绍了直线感应电机驱动电梯的优点和不同结构类型，对传统的v／f控制，基于空间矢量法的v／f控制，矢量控制，和基于电压空间矢量的直接转矩控制进行了比较，确立了本课题的研究主题：直线感应电机双边直推式驱动电梯的全数字变频控制。

The article combines with the treatment of a superficial civil air defense work to introduce how the grouting method to improve the performance of the backfill soil.

文章从治理漂浮人防工事的角度提出了注浆技术在改善回填土性质方面的应用，并详细阐述了注浆技术的施工流程。