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The purified N protein was used to coat 96-well plate, each step was optimized, such as coating concentration of recombinant nucleocapsid protein, scample dilutim, chromogen and stop solution as well as concentration.

在此基础上，以纯化的重组N蛋白作为包被抗原，对各种条件进行优化（如抗原的包被，作用时间及底物的选择），确定了判定标准，建立了检测PRRS抗体的间接ELISA方法。

It was found that the change of surface potential of the purple membrane resulting from acetylation induced the change of local protein conformation Which could be delocalized all over the protein to a great extent and affected the nature of binding sites of retinal and the structure of the retinal chromophore.

结果表明：酰化引起的表面电位的改变诱导了蛋白局部构象的改变。这种改变在很大程度上离域到整个蛋白分子中而影响了视黄醛结合位点的性质及生色团的结构，从而导致可观察的光谱变化。

The long-term opening of mitochondria permeability transition pore results in the release of cytochrome C, apoptosis inducing factors, Ca2+ and other apoptosis factors into cytoplasm, which can destruct the whole cell structure and function by activating the main member of apoptosis protein family——caspase, or destroying the intranuclear chromoplasm independently, or affecting other protein relying on Ca2+, and finally lead to the formation of apoptotic body.

线粒体是真核生物能量和代谢的中心，也是细胞凋亡信号传导途径中起关键作用的细胞器，越来越多的研究表明，在凋亡信号的刺激下，线粒体发生膨胀，其外膜膜电位降低，外膜破裂后释放出膜间隙的诸多凋亡效应因子，在细胞凋亡中起着主开关的作用。

In this article, we studied some components in giant panda seminal plasma and their relationship with semen characteristics.The protein bands were separated by SDS-PAGE, platelet-activating factor acetylhydrolase activity and glutamic-aspartic aminotransferase activity were detected by ELASE and chymic colorimetry respectively. The results are as followed:Twelve protein bands in giant panda seminal plasma were identified by SDS-PAGE, the molecular weights were 74.9、59.6、 56.2、 54.7、 47.3、 38.2、 30.8、 23.8、 18.4、 16.8、14.0 and 13.4kDa respectively, six of them (74.9, 59.6, 56.2, 47.3, 30.8 and 13.4kDa) werepresent in all samples. However, there was no relationship was found between the abnormalprotein bands appeared in several samples and semen characteristics in our experiment.

本研究采用SDS-PAGE对大熊猫精浆蛋白电泳谱带特性进行分析，使用酶联免疫方法检测精浆血小板激活因子乙酰水解酶活性、化学比色法检测精浆谷草转氨酶活性，并初步探讨了这些物质与大熊猫精液质量的关系，结果显示：(1)大熊猫精浆SDS-PAGE电泳共分离得到12条蛋白谱带，其分子量分别为74.9kDa、59.6kDa、56.2kDa、54.7kDa、47.3kDa、38.2kDa、30.8kDa、23.8kDa、18.4kDa、16.8kDa、14.0kDa和13.4kDa，其中74.9kDa、59.6kDa、56.2kDa、47.3kDa、30.8kDa、13.4kDa谱带为所有个体大熊猫精浆样品所共有。

The optimal conditions of the pepsin hydrolysis of frame protein in catfish Claris lareza were investigated using a general-purpose rotation unitized design of the second power of four factors, and the content of soluble peptides was determined in the hydrolytic products. The Optimization of Design Expert 7.1.2 revealed that antibacterial activity of the frame protein hydrolysates was controlled by regulation of four process conditions.

采用四因子二次通用旋转设计，以抑菌率为测定指标，对胃蛋白酶水解革胡子鲶Claris lareza鱼骨蛋白的条件进行了优化，同时测定了酶解产物中可溶性肽的含量，以分析抑菌率与可溶性肽含量之间的相关关系。

The recognition mechanisms of GGR and TCR to UVB-induced photodamages were summarized: Xeroderma Pigmentosum group C, the recognition factor in GGR, executed its function avoiding direct contacting with lesions; the conversion between monomer and dimer of Cockayne syndrome B protein was underlined in the recognition of TCR; ubiquitylation mediated by DNA damage binding protein played an important role in NER recognization at chromatin level; moreover, the fate of the recognition factors was also discussed.

识别因子XPC通过一种不直接识别损伤本身的机制在GGR识别过程中发挥作用；在TCR识别过程中强调了关键因子CSB单体及二聚体两种形式的转换。在染色质水平上，DDB介导的泛素化作用是NER识别过程中重要的调控要素。另外，完成使命的识别因子的最终走向也是NER途径中的一个重要环节。通过分析上述生化过程，较清楚地总结了GGR及TCR对UVB导致的光损伤的识别机制。

Many studies show codon usage in protein-coding sequence is relevant to tertiary structure of the protein.

很多研究表明蛋白质编码序列中密码子的选择和蛋白质结构相关。

CWhen the fusion protein was co-culture with nematode,the analysis of fluorescence microscope showed the digestive system and the coelom of nematode could be detected green fluorescence, and the fluorescence intensity was time-and protein concentration-dependent.

其三，融合蛋白喂食线虫发现蛋白进入其整个消化道及其原体腔，且与喂食时间与蛋白浓度呈正相关关系。

In particular, transgenic maize expressing higher levels of a protein designed to exhibit increased toxicity toward Coleopteran pests deliver superior levels of insect protection and are less likely to sponsor development of populations of target insects that are resistant to the insecticidally active protein.

特别是，设计一种蛋白以表现出对鞘翅目害虫提高的毒性，表达较高水平的这种蛋白的转基因玉米表现出优越的昆虫防治水平，并不太可能引起产生抗所述杀虫活性蛋白的目标昆虫群体。

Using the obtained antiserum as the first antibody, and the monoclonal antibody 4C11 as the collator, the specificity of the antiserum was tested by Western blotting and indirect-ELISA with the Yak recombinant mature PrP, GST fusion protein, the extract of brain and the bacterium protein.

以自制抗血清为一抗，单抗4C11为对照，对牦牛重组成熟朊蛋白、GST蛋白、脑组织提取物和菌体蛋白进行Western blotting检测和间接ELISA试验，验证自制抗血清的特异性。

As of Tuesday, Google's results were still censored in China.

截至周二，谷歌的搜索结果仍受中国审查。

In order to make the positive action increase and negative one decrease, the sub-forces of the social factors must be adjusted to form a centripetal force.

在这一过程中，人的主体性发挥是社会有机体健康发展的灵魂。