查询词典 protein

When total protein was assayed by 2-D PAGE andstained with sliver, about 2000 protein spots with a pI of pH 3-10 and Mrof 12-120 kDa could be detected. All these protein spots were excised foridentification, among which 213 spots corresponding to 192 differentproteins were identified undoubtful by searching the mass spectra againstthe SwissProt/TrEMBL database.

我们利用双向凝胶电泳对小鼠卵巢蛋白进行了分离，在考马斯亮兰染色后将所有可见的蛋白点切取下，经胰蛋白酶消化以后用MALDI-TOF质谱仪进行鉴定。

Our results indicate that it is essential to consider electronic polarizations of the substrates in order to understand different binding affinities in protein-protein and protein-ligand binding.

我们的结果表明在对于蛋白质和蛋白质以及蛋白质和配体的结合自由能，结合强度的研究中，考虑极化效应的影响是必须的。

Imbalanced protein andprotein supplied in excess of maximal daily retention rate of protein is deaminated and thenet yield of energy can be considered to join the protein-free digestible energy intake togive a flow of metabolizable energy.

研究表明，对同一品种而言，公鸡的自由采食量一般要高于母鸡；对同一性别而言，则现代的品种要高于较早的品种。本文还提出了适用于现代品种肉仔鸡的自由采食量预测公式。

In this study, we cloned the BFV gag gene into prokaryotic expression vector pET28a and purified the denaturalized Gag protein. The protein was used to immunize BALB/c mouse to produce antiserum, which could specifically recognize the BFV Gag protein in BFV-infected cells through western blot assay.

本文将BFV gag基因克隆入原核表达载体pET28a中，纯化得到了变性的Gag蛋白，用于免疫BALb/c小鼠获得抗血清，进行了Western blot杂交和免疫荧光实验。

Effects of microbial transglutaminase on the tensile properties of plastic made from soy protein isolates are studied. The results show that the tensile strength and Young modulus of the protein plastic are increased from 9.2MPa and 265.4MPa of the control, which is not treated with TG, to 14.7MPa and 390.1Mpa respective, which is treated with TG at 50℃ for 60min. High molecular weight protein conjugates are formed with TG treatment, and they are responsible for the enhanced tensile properties, denaturalizing temperature and enthalpy values.

研究了微生物谷氨酰氨转胺酶对大豆分离蛋白塑料拉伸性能的影响，发现在50℃时转氨酶对大豆分离蛋白催化聚合反应60 min，形成的高分子聚合物使大豆分离蛋白的抗拉强度和杨氏模量分别由未经处理时的9.2 MPa和265.4 MPa增加到14.7 MPa和390.1 MPa，同时增加了蛋白质的变性温度和热焓值。

In Part 1 of our research, BGSCs were sorted through immunomagnetic beads marking by CD133 and cultured in vitro, and character as a stem cell was identified by stem cell markers (CD133 and Nestin) and differentiated cell markers [ microtubule-associated protein 2(MAP2), glial acidic fibrillary protein and myelin basic protein] , ultrastructure observing with electron microscopeand engrafting to severe combined immunodeficiency mice for tumorigenesis test. The results were as following: Only a small subset of CD133+ glioma cells in glioma cell lines and fresh specimens from various pathologic grade could express stem cell markers CD133 and Nestin, view ultrastructure of a stem cell and be capacity of serial passage in culture. These CD133+ cells possese a marked capacity for multipotent differentiation and could differentiate into tumor cells expressing MAP2,β-TubulinⅢ, GFAP and MBP; When engrafted into SCID mice, they can generate and form tumors that phenotypically resembl the tumor from the patient.

在本课题中，在第一部分实验中采用以CD133为标志的免疫磁珠法从人脑胶质瘤组织和细胞株中分离脑胶质瘤干细胞并进行体外培养，通过免疫荧光技术检测干细胞标志物CD133、Nestin，诱导分化后检查分化细胞标志物MAP2、GFAP、MBP以及电镜超微结构观察和移植SCID鼠致瘤实验，对其干细胞特性加以鉴定，得到如下结果：不同病理分级的新鲜胶质瘤标本和胶质瘤细胞株中存在一小部分CD133+的胶质瘤细胞，能表达干细胞的标志物CD133和Nestin，符合干细胞的超微结构特点，体外培养能连续传代；具有多向分化潜能：诱导分化后能产生MAP2、β-TubulinⅢ、GFAP、MBP染色阳性的细胞；移植SCID鼠后能形成与亲本肿瘤表型一致的移植瘤。

The shorter variant mHemk2 is 1696 bp in length encoding a putative mouse protein of 14.7 kDa, consisting of 138 amino acid residues.In protein data bank annotations, Hemk has previously been classed among methyltransferases methylating N6-adenine or N4-cytidine in DNA because of the presence of S-adenosyl-L-methionine binding motif and of an NPPY motif in the protein.

在蛋白数据库的注释中，由于在Hemk蛋白中存在S-腺苷-L-甲硫氨酸结合基序和一个NPPY基序，因此，在蛋白库的说明中已经将Hemk蛋白归类于能甲基化DNA中N6-腺嘌呤或N4-胞嘧啶的甲基转移酶。

The results showed that spring wheat with plastic film mulches was able to improve the content of chlorophyll and protein of leaves in the earlier growing period and the average increase per day of the content of chlorophyll and protein of the mulched wheat increased by 31.49% and 7.94% than that of unmulched wheat respectively; but it could accelerate the decomposition of chlorophyll and protein in the later period.

结果表明，采用地膜覆盖栽培方法，在春小麦生长前期能明显提高叶片叶绿素和蛋白质的含量（日均增加量分别比露地春小麦高31.49%和7.94%），但后期又会加速其降解速率；MDA含量和细胞质膜相对透性均表现为覆膜处理高于露地处理（日均增加量分别比露地春小麦高71.96%~11.78%和2.42%~9.57%）。

The expression amount of the target protein is improved by three to ten times according to different target protein, different multiplicities of infection, different protein harvesting times and different consumptions of culture medium.

依不同目的蛋白，不同感染复数，不同蛋白收获时间及不同培养基使用量，目的蛋白表达量增加3～10倍。

BACKGROUND: Protein kinase A signaling has long been known to play an important role in cardiac function. Dysregulation of the protein kinase A system, caused by mutation of the protein kinase A regulatory subunit gene PRKAR1A, causes the inherited tumor syndrome Carney complex, which includes cardiac myxomas as one of its cardinal features.

背景：长期以来，蛋白激酶A信号途径被认为在心功能中起着重要作用，蛋白激酶A调节亚单位基因PRKAR1A的突变导致蛋白激酶A系统失调，引起遗传性肿瘤综合征－Carney合成，其中心脏粘液瘤作为其主要特征。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。