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Inhibition of PKCdelta by rottlerin or knockdown of TG2 protein by a TG2-specific siRNA resulted in a marked increase in autophagy shown by presence of autophagic vacuoles in the cytoplasm, formation of the acidic vesicular organelles, membrane association of microtubule-associated protein 1 light chain 3 (LC3) with autophagosomes, and a marked induction of LC3-II protein, important hallmarks of autophagy, and by electron microscopy.

抑制PKCdelta的rottlerin或击倒的TG2蛋白质的TG2特定的siRNA导致显着增加，表明自噬存在自泡在细胞质，形成酸性囊泡细胞，膜协会微管相关蛋白1轻链3（ LC3 ）与autophagosomes ，并显着诱导LC3 -Ⅱ蛋白的重要标志吞噬，并通过电子显微镜。

The result of BLASTx showed that Maasr1 was homologous with Oryza sativa abscisic acid-inducible and stress-inducible protein (Asr1) mRNA (86%),Saccharum officinarum SoDip22 mRNA for drought inducible 22 kD protein (84%),Lilium longiflorum anth (A23) mRNA (86%), and Prunus armeniaca abscisic stress ripening protein homolog mRNA(82%).

用Maasr1基因作BLASTn比对发现它可能与水稻ABA和胁迫诱导蛋白(ASR1)的mRNA有86%的同源，与甘蔗干旱胁迫蛋白(SoDip22)的mRNA有84%的同源，与百合花A23基因的mRNA有86%的同源，与野黑杏脱落胁迫成熟蛋白的mRNA有82%的同源。

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

In short, this paper two methods of protein extraction studies, the initial establishment of the Tenebrio molitor protein extraction methods and the best technology, the development of Tenebrio molitor for the future of protein resources to provide a theoretical basis.

总之，本论文通过两种蛋白质提取方法的比较研究，初步确立了黄粉虫中蛋白质的最佳提取方法和工艺，为以后开发黄粉虫蛋白质资源提供了理论基础。

NodD binds to and bends target promoters through anchoring two tandem and individual specific DNA sites. NodD functions as a tetramer, which has a V-shaped main body. Tetrameric NodD is to change its own conformation rather than its oligomeric forms in response to small signal molecules. The specific interaction between each NodD DNA-binding domain and each specific DNA site does not alter itself in spite of naringenin induction, and the induced conformational change is transferred from protein to DNA. Only the DNA conformation incited by induced NodD is competent for RNA polymerase to form the transcriptional open complex. It cannot be excluded that NodD may have protein-to-protein contacts with RNA polymerase, and that the NodD conformational change may also directly contribute to the transcriptional open complex formation. However, the NodD conformational change itself cannot serve as the determinant of the transcriptional molecular switch.

通过研究，我们提出了初步的NodD操纵子激活模型：第一，四聚体是NodD蛋白的功能单位，它通过铆钉两个串联的相对独立的DNA靶位点结合被诱导的启动子；第二，小分子配基的结合是改变NodD四聚体的构象而不是引发不同形式的寡聚体，在我们的模型中，NodD四聚体缩小其V形主体的弯折角，进而缩短其DNA结合功能域的间距；第三，小分子信号的诱导并没有改变NodD的DNA结合域和其DNA靶位点的相互作用，NodD的构象改变由蛋白质经其双铆钉位点传递给DNA；第四，只有诱导状态的NodD激发的DNA构象才能有效地使RNA聚合酶形成转录开放复合物；第五，不排除NodD与RNA聚合酶可能有直接的相互接触位点，不排除NodD构象的改变可能直接有利于RNA聚合酶形成转录开放复合物，但是我们认为NodD构象改变本身不是充当转录激活开关的决定因素。

The binding behavior and kinetics of uncoupling protein with its ligand, the Fourier-transformation infrared spectra of uncoupling protein and its changes during ligand-binding process, the inhibition of Guanosine 5＇-Tetraphosphate (G-4-P) and Adenine-β-Darabinofuranoside 5＇-triphosphate on the binding of uncoupling protein with GTP were studied.

本文对解偶联蛋白与配基的结合及其结合动力学，解偶联蛋白及其与配基结合过程中的傅里叶变换红外光谱及其光谱变化，鸟嘌呤核苷四磷酸（G-4-P）和9-β-D-阿拉伯糖基呋喃5＇-三磷酸对解偶联蛋白与GTP结合的影响进行了研究。

SGK is a novel member of serine/threonine protein kinase family that is transcriptionally regulated. As an adaptor or scaffold protein, 14-3-3 protein plays important role in a variety of signal transduction pathways.

SGK是丝氨酸／苏氨酸蛋白激酶家族的一个新成员。14-3-3蛋白作为榫头蛋白或支架蛋白，在许多信号传导途径中起重要作用。

Differentially expressed protein spots were identified by Tandem MS as RNA binding protein regulatory subunit, Proteasome subunit beta type 7 precursor, and Translationally controlled tumor protein.

进一步选择了3个组间差异表达蛋白点进行串联质谱分析，分别鉴定为RNA结合蛋白调节亚基、蛋白酶体β亚基7型前体和翻译调控肿瘤蛋白。

SUMO has been shown to covalently modify a large number of proteins, reversible modification by SUMO regulates nucleocytoplasmic translocalization, protein-DNA binding activity, protein-protein interaction, transcriptional regulation, DNA repair, and genome organization.

SUMO与靶蛋白之间这种可逆的共价连接，在核质运输、DNA 与蛋白质结合活性、蛋白质之间相互作用、转录调控、DNA 修复以及维持基因组稳定等方面均发挥着重要的调节作用。

Therefore,a sequence specific artificial zinc finger proteinis created when replacing the amino acids in the specific sites of C2H2 zinc finger protein and then fusing the protein to other activation or repression domains,while remaining the backbone of the zinc finger protein unchange...

ZFP可以介导靶基因的转录调控，抑制或激活特定基因的表达与配体依赖的靶基因激活或抑制；对DNA进行修饰，如人造限制性内切酶，重组酶，整合酶；抗病毒感染等。因此，人造锌指蛋白应用前景广阔，研究价值显著，是未来人类基因治疗的革命性的工具。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。