查询词典 protein-rich

Calpain system is probably the major proteolytic in protein degradation,which plays an important role in myofibrillar protein degradation.

近年的研究表明，calpain是细胞质中主要的蛋白水解酶，在肌原纤维蛋白降解中起着重要的作用。

The ASK1 (ARABIDOPSIS SKP1-LIKE) protein is a critical component of the SCF (Skp1-Cullin-F box protein) ubiquitin ligase complexes that recruit target proteins for degradation by the 26S proteosome.

这些研究丰富了人们对nsLTP基因功能的认识，为进一步克隆其受体基因并最终阐明nsLTP介导的信号传导途径奠定了基础。

Effect of protein and lipid in rice on starch physicochemical characteristics, difference between the region of non-chalkiness and chalkiness of rice, dynamic variation of cell structure and CaM distribution on rice spike were expounded in this study. It was found that protein and lipid in rice had important effect on starch gelatinization, there were distinct differences between the region of non-chalkiness and chalkiness of rice, vascular bundle system in rice spike was changed during grain filling, difference quality of different position of rice spike was more distinct in dense spike rice for more powerful vascular bundle system, the development of rice seeds was corresponded to pericarp development, difference quality of different position of rice spike was also related to transport function of rachilla besides activity of "source", and calmodulin relative activity was closely related to grain filling velocity and enginery properties.

通过实验发现：稻米中蛋白质、脂肪对淀粉的糊化过程有着较大的影响；精米的非垩白与垩白部位以及心白与腹白部位之间在蛋白质、氨基酸含量及组成方面存在着明显差异：水稻穗部大维管束面积以及主穗轴维管束数目在灌浆过程中存在明显的动态变化趋势；密穗型水稻强势粒的维管组织比散穗型水稻发达，致使其强、弱势粒维管组织差异明显加大；果皮的发育与籽粒发育状况是相适应的：强势粒和弱势粒在灌浆生理方面的差异，不仅表现在籽粒本身的&库活性&上，而且也与其着生小穗轴的输导功能有关；CaM相对活性与水稻灌浆速率、机能特征间存在着密切联系。

Reen fluorescent protein-CPK30 (GFPCPK30) fusion protein studies revealed the localization of AtCPK30 to both cell wall and plasma membrane.

FP-CPK30融合蛋白的亚细胞定位研究结果表明：CPK30蛋白定位在细胞壁和细胞膜上。

With ultrasonic wave and lysozyme, the recombination N protein is split from bacteria. We dissolve it in guanidine hydrochloride, purify it with Ni〓 affinity chromatography column and renature it in vitro. By testing the antigen of the purified N protein, it shows that its activity is very high.

建立了用超声波和溶菌酶裂解菌体，用盐酸胍溶解，Ni〓亲合层析柱纯化及体外复性等方法，经检测证明纯化的VSV重组核蛋白抗原具有较高的活性。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

The identified proteins are involved in a variety of cellular process including several zinc finger proteins relevant to transcription regulation, such as zinc finger 198, 263, 14, 224, zf6, novel protein similar to transcriptional represser CTCF, and kruppel-like zinc finger protein; two members of the ADAMs (a disintegrin and metalloprotease domain) family; two members of integrin family; several proteins involved in the signal transduction, cell-cycle control, chromatin remodeling and transcription repression; and also some proteins of cell skeleton and some with unknown functions.

鉴定出 的蛋白包括多个与转录调控相关的锌指蛋白，如锌指蛋白198、263、14、224、zf6、转录抑制因子cTcF样蛋白、幻肚pple样锌指蛋白等：两个含金属蛋白酶结构域和整合素结合结构域的家族成员ADAM28和ADAM17；两个整合素家族成员pZ整合素和含十个EGF样结构域的整合素；与细胞信号转导通路有关的蛋白；与细胞周期调控有关的蛋白；与染色体重塑和基因转录抑制有关的蛋白；细胞骨架蛋白以及其他功能未明的蛋白等。

Amino acid mutant site A43V locates in N-terminal headpiece of represser protein which binds specifically to DNAand amino acids mutant sites S93L, S102N, G315S, Q180R, M2491, I283T all locate in the core region of represser protein which binds to IPTG.

氨基酸突变位点A43V位于阻遏蛋白氨基末端，是阻遏蛋白和操纵基因的结合区；S93L、S102N、G315S、Q180R、M249I、I283T均位于阻遏蛋白核心区，即阻遏蛋白和IPTG结合区。

Based on the idea of coarse-grained description in physics, a new encoding method with grouped weight for protein sequence is presented, and applied to protein structural class prediction associated with component-coupled algorithm. The average rate of correct recognition is 99.72% in Resubstitution test and 91.09% in Jack-knife test for standard set of 359 proteins.

从氨基酸的物化特性出发，利用物理学中&粗粒化&思想，提出了一种蛋白质序列的分组重量编码方法（Encoding Based on Grouped Weight，简记为EBGW），并结合组分耦联算法进行结构型预测的研究。

Several methods of plant tissue protein extraction (TCA-acetone precipitation method, phenol method, urea method, Zhou Han-tao described method, and phenol method improved by the authors) were applied to extract total protein from Rhizophora stylosa root, and then after SDS-PAGE, a subsequent 2-DE analysis was carried out with the optimized conditions for 2-DE .

分别采用TCA-丙酮沉淀法、酚法、尿素法、周涵韬法和笔者建立的酚改良法，提取红海榄根部总蛋白质，并进行了SDS-PAGE分析和双向电泳体系的优化。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐