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protein-rich相关的网络例句

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METHODS: NE has been shown to be a potent stimulator for neonatal rat ventricular myocyte hypertrophy in vitro. We used the activator of PPAR γ, the inhibitor of protein kinase C chelerythrine to influence protein content.3H?

在培养新生大鼠心肌细胞中,采用RSG,PKC的激动剂佛波醇酯,NE和PKC的阻断剂白屈菜季氨碱观察罗格列酮在NE和PMA诱导心肌肥大中对PKC活性和c?

However it was shown its eventually inhibiting effect on apoptotic myocyte cells during I〓 R〓h (P<0.05); The PKC inhibitors chelerythrine and scutellarein have no effect on expression of Fas protein, whereas they can significantly upregulate the expression of Bcl-2 protein.

4PKC激活剂PMA可下调Fas基因的蛋白表达,对Bcl-2蛋白的表达也有抑制作用,它对缺血30min、再灌注24h后心肌凋亡细胞的最终影响为抑制效应(P<0.05),而PKC抑制剂CHE、灯盏花素对Fas基因蛋白表达无明显干预影响,但对Bcl-2蛋白表达呈显著上调作用,故对心肌细胞凋亡的抑制作用更为明显(P<0.01)。

The 20-HETE-induced increases in [Ca2+]i, whole-cell L-type Ca2+ channel currents and the amplitudes of Ca2+ transients were blocked by extracellular application of chelerythrine (an inhibitor of protein kinase C, PKC) and H-89 (an inhibitor of cAMP-dependent protein kinase, PKA).

上述20-HETE诱导的[Ca2+]i、全细胞L-型钙离子通道电流、钙瞬变幅度的增加等效应,可被蛋白激酶C抑制剂白屈菜红碱和cAMP依赖蛋白激酶抑制剂H-89阻滞。

Then the reaction rates of IgE and IgG in asthmatic patients serum to chenopodium album pollen were counted by immunoblotting. RESULTS: Twelve bands of protein were obtained through SDS PAGE. The molecular weight of each band was 92, 63.1, 61, 52, 45, 43, 39.7, 38.9, 34, 31.6, 28.4 and 18.5~12 ku. Immunobloting identified 4 protein bands recognized by sIgE, molecular weights being 92, 34, 31.6 and 18.5~12 ku, which reacted with 70%, 50%, 80% and 90% of asthmatic patient serum.

结果: 藜草花粉经电泳后得到12条蛋白质区带,分子质量依次为92, 63.1, 61, 52, 45, 43, 39.7, 38.9, 34, 31.6, 28.4和18.5~12 ku;免疫印迹可见4条sIgE反应带,蛋白分子质量依次为92, 34, 31.6和18.5~12 ku;与患者血清反应率分别是70%, 50%, 80%和90%;与血清sIgG结合的反应带有8条,蛋白分子质量为92, 61, 52, 45, 43, 39.7, 31.6和18.5~12 ku;与患者血清反应率分别是80%, 40%, 10%, 20%, 80%, 10%, 10%和100%。

The expression of the chimeric protein was shown by fluorescent microscope. The chimeric protein only existed in cytoplasm.

转染HEK293细胞后,荧光显微镜检测显示融合蛋白仅在细胞浆中表达。

Select chorioallantoic membrane vascular extract protein of different time points , Detect FGF2 and TGF-β2 protein in chorioallantoic membrane of this three-stage by Western blotting, judge whether to establish a model of dynamic vascular development successful.

选取不同时间点的尿囊膜血管,提取蛋白,用Western blotting技术检测FGF2和TGF-β2蛋白在血管发育的这三个阶段的表达量的变化,判断动态血管发育模型是否建立成功。

Methods The plasma levels of protein C activity and protein C antigen were measured using chromogenic assay and ELISA, respectively.

分别用发色底物法和ELISA测定血浆蛋白C活性和抗原。

OsCH2 protein in rice has 85%,57%,57%,60%, 58% and 61% homologous withzea, gentiana, coffea, lycopersicon, citrus and crocus respectively. OsHSP82 protein has 95%,94%,90%,90% and 89% homologous with wheat,barley, tobacco,tomato and arabidopsisrespectively.

水稻OsCH2蛋白与玉米、龙胆草、阿拉比卡咖啡豆、番茄、甜橙、番红花蛋白同源性分别为85%、57%、57%、60%、58%和61%,OsHSP82蛋白与小麦、大麦、烟草、番茄和拟南芥蛋白同源性分别为95%、94%、90%、90%和89%。

Recombinant adenovirus was identified by polymerase chain reColon cancer cell line SW480 was infected with recombinant adenovirus Ad-p27mt, and expression of p27 protein was detected by Western blot.of expressed p27 protein after Ad-p27mt transfected colon cancer cell.novirus framework plasmid pAdeasy-1 with pShuttle-CMV-hp27mt, 30%combinant adenovirus DNA contained the target gene.

Ad-p27mt的聚合酶链反应检测Ad-p27mt转染大肠癌细胞后的p27mt的表达。结果:①用含pShuttle-CMV-hp27mt转化含pAdeasy-1的超感受态BJ5183后,可获得约30%的阳性重组质粒。②聚合酶链反应检测表明重组腺病毒DNA中含有目的基因。重组腺病毒滴度为7.95×1015pfu/L。

Using the intracellular recording technique for monitoring the excitatory postsynaptic potentials of CA1 pyramidal neuron and delivering an activator of protein kinase C and/or inhibitors of PKC and 〓/calmodulin-dependent protein kinase Ⅱ into postsynaptic cell in hippocampal slices, we studied the role of postsynaptic PKC and CaMKⅡ in long-term potentiation induced by high frequency stimulation at the CA1 synapses made by Schaffer collateral/commissural and perforant pathway afferents.

用电生理学方法在大鼠海马脑片CA1区锥体细胞记录Schaffer侧枝和穿通通路到CA1神经元突触的兴奋性突触后电位,通过送蛋白激酶抑制剂和激活剂进入突触后细胞,我们研究了突触后蛋白激酶C和依赖钙/钙调蛋白的Ⅱ型蛋白激酶在高频刺激诱导的长时程增强产生和维持中的作用。

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