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The N protein concentration in the supernatants of virus-infected cells were reduced at different levels in all ER and cytoplasma localized intracellular antibody expressing cell lines, however, the intracellular N protein synthesis in above cell lines was not significantly affected.

利用病毒微量滴定免疫荧光方法,对细胞内抗体与病毒的增殖关系进行了研究,证实无论在内质网还是在胞质,抗核蛋白细胞内抗体都能够抑制病毒的增殖,具有抗病毒活性。

The optimal diagnostic model was developed by support vector machine algorithm with two protein peaks at Mass/Charge 3939 and 5909 respectively. The expression of protein peak at m/z 3939 was higher in chronic eczema and neurodermatitis than in MF, while that at m/z 5909 appeared to express in an opposite way.

结果 从15例蕈样肉芽肿与15例慢性湿疹、神经性皮炎患者的血清蛋白质谱中共检测出329个蛋白峰,从中筛选出30个表达差异有统计学意义(P 0.01)的蛋白标志物。

Nighty rats were randomly divided into the groups of control, myocardial infarction and doxycycline, which were given orally 48 hours before and 48 hours after MI with 30mg/Kg per day. Protein and mRNA extraction was done on left ventricular samples containing scar and myocardium together. Samples were assayed from 1 day to 4 weeks post-Mi. The activity of MMPs was measured by zymography and collagen amount by the method of chloramine T, the ratio of II III collagen was assessed by immunohistochemical stain. Protein and mRNA of MMP-2, 9, TIMP-1 were determined by Western-Blot and RT-PCR.

分为对照组、心梗组和治疗组(D组,术前两天至术后两天,口服Doxycycline,30mg/kg/天),分别于术后第一天、术后一周、术后两周、术后四周取心肌组织,采用免疫组化测定胶原含量和Ⅰ/Ⅲ胶原比例,酶谱法测定心梗后MMP-2,9活性蛋白的表达规律,Western Blotting进一步确定酶谱法中所消化条带蛋白的属性,逆转录-聚合链反应法测定心梗后MMP-2,9和TIMP-1的mRNA的变化规律,运用Acuson Sequoia 512超声心动图机分别测定心梗组、治疗组在两周和四周时左室前壁、后壁厚度以及左心室舒张末内径和左室射血分数。

In this paper, the complete nucleotide sequence of watermelon mosaic virus Chinese Isolate was determined. On the basis, the HC-Pro protein and CP protein both are the factors for aphid transmission were cloned and expressed in vitro, and their antiserum were prepared. By far western blot, viral receptors were identified in aphids, and their interactions were studied. The molecular mechanism of nonpersistent transmission was further elucidated.

本文完成了我国西瓜花叶病毒的基因组全序列的测定,在此基础上,克隆和体外表达了蚜传因子HC-Pro和CP蛋白,制备了相应抗体,利用Far western blot技术鉴定了其蚜虫体内的受体蛋白,并对三者互作关系进行研究,进一步阐明了非持久性传毒的分子机制。

Ellman reagent was used to determine the contents of nonprotein SH, total protein SH and total protein -bound SH in the above lens.

方法44个人类晶体按年龄大小被分为5组,其中10个白内障晶体被分成2组。

mdm2 gene expression atmrna and protein levels,p53 protein and eb virus dna were detected by nonradioactive in situ hybridization ,immunohistochemistry and polymerase chain reaction separately in 46 cases of npc tissuesand 12 cases of chronic inflammation of nasopharyngeal epithelium.

运用非同位素原位杂交、免疫组化和多聚酶链反应技术对46例npc、12例慢性鼻咽炎症粘膜分别进行mdm2 mrna、mdm2蛋白、p53蛋白及ebv-dna的检测。

Results: No protein peak was detected in thirty urine samples of the control group, hut protein peaks were detected in seventy urine samples of the case group with the exception of two and among them 16 were selective proteinuria, 50 were nonselective proteinuria and 2 were overflow proteinuria.

用微流控芯片电泳对30例尿蛋白定性试验为阴性的对照组和70例尿蛋白定性在++以上的病例组进行检测,以白球蛋白的峰面积比值判断蛋白尿的选择性,并与美国Helena琼脂糖凝胶电泳结果比较。

Results:No protein peak was detected in thirty urine samples of the control gruop, but protein peaks were detected in seventy urine samples of the case group with the exception of two and among them 16 were selective proteinuria, 50 were nonselective proteinuria and 2 were overflow proteinuria.

用微流控芯片电泳对30例尿蛋白定性试验为阴性的对照组和70例尿蛋白定性在++以上的病例组进行检测,以白球蛋白的峰面积比值判断蛋白尿的选择性,并与美国Helena琼脂糖凝胶电泳结果比较。

Total protein decreases because the objective is to remove as much unwanted or nonspecific protein as possible.

蛋白质总量的减少是因为要尽可能地移除不想要的或非特定蛋白质的目的。

AIM: To investigate the expression of runx3 gene mRNA and protein in human gastric carcinoma and surrounding normal tissue and its correlation with clinicopathological parameters. METHODS: RNA and protein were extracted from 60 human gastric carcinoma and surrounding normal tissue specimens.

采用逆转录多聚合酶链反应和Western Blot技术对临床60例胃癌新鲜标本及其周围正常粘膜组织中runx3基因和蛋白的表达进行检测,并对其表达和各临床病理参数的关系进行分析。

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