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It was found from the study that selenium and protein contents in foxtail millet landraces germplasms exhibited wide genetic diversity, some foxtail millet landraces with higher selenium and protein contents were identified and they will be useful for quality breeding and germplasm innovation.

谷子品种资源的硒和蛋白质含量存在较为丰富的遗传变异,在鉴定评价基础上,筛选出了一批品质优异的谷子种质资源,为富硒和高蛋白优质品种的培育和种质创新奠定了基础。

Selenium and protein contents were analyzed and evaluated in foxtail millet landraces originated from different regions of China. The method for selenium determination was Hydride Generation Atomic Fluorescence Spectrometry, and the method for protein determination was semi-micro Kjeldahl method.

对中国谷子地方品种资源进行硒和蛋白质含量的测定与评价,以了解谷子品种资源中硒和蛋白质含量的变异及其与地理分布的关系,为谷子种质创新和育种提供基础。

The main results are shown as follows:1.Screening freezing tolerant cultivars and lines of chrysanthemumTwenty seven lines of chrysanthemum grown outdoors were screened using \'injury rate\' and \'injury index\'.There were 7 cultivars and lines,which were relatively high tolerant to freezing treatment,i.e.,\'Jinbuhuan\',\'Xuetao\',\'Fuli\',\'Donglin 4\',\'Donglin 6\',\'Donglin 9\', and \'Donglin 13\'.There were 3 cultivars and lines,which were less tolerant,i.e.,\'Fenmeigui\', \'Donglin 1\',and \'Donglin 2\';The determination of electrolyte leakage,and contents of free proline,MDA,CAT,fructose,glucose and soluble sugars on 7 lines of chrysanthemums at different stages freezing showed that \'Xuetao\' and \'Donglin 4\' were more tolerant than others, and that especially \'Donglin 4\' was able to regulate its osmotic pathway to alleviate the \'injury degree\' caused by freezing conditions.2.Protein expression profile of chrysanthemum under low temperature stressTwo-dimensional liquid chromatography was applied to fractionate and analyze total protein taken from \'Donglin 4\' leaves grown at low temperature and at normal condition. Chromatography peaks of fraction were transformed to gel graph by ProteoVue software.

主要研究结果如下:1、露地菊抗冻品种和品系的筛选利用受伤率和伤害指数两项指标对27个露地菊品系进行耐低温筛选,筛选出'金不换\'、'雪涛\'、'富丽\'、'东林4号\'、'东林6号\'、'东林9号\'、'东林13号\'等7个耐低温品系,同时发现'粉玫瑰\'、'东林1号\'、'东林2号\'三个品系的耐低温能力最差;通过对不同自然低温处理时期,7个品系的电导率、丙二醛含量、脯氨酸含量、过氧化氢酶、果糖、葡萄糖和可溶性糖等指标的测定发现,'雪涛\'和'东林4号\'的耐低温能力要强于其它品系,尤其是'东林4号\'对低温的调节能力较强,在低温胁迫下,能够调节渗透途径减轻低温的伤害。2、低温胁迫下,露地菊蛋白质表达谱分析利用二维液相色谱技术,分离了'东林4号\'露地菊品系的低温处理和对照样品的总蛋白,通过ProteoVue软件分析发现:处理样品与对照相比共有蛋白差异点36个,其中显著增强表达的蛋白差异点有26个。

Objective To study the effects and significance of Bax protein and heat shock protein 70 (HSP70) in prevention of insulitis and diabetes by complete Freund's adjuvant in female NOD mice.

目的 研究Bax蛋白和热休克蛋白70在完全弗氏佐剂预防NOD鼠胰岛炎和糖尿病中作用及意义。

This technique was modified as follows: the small rubber particles were purified and diluted to give a protein concentration of an absorbance at 600 nm of about 2.0~2.5; 0.5% basic fuchsin was prepared by being dissolved in 50 mmol/L Tris-HCl(pH7.4); the reaction mixtures were incubated for 30 min at 25 ℃, and centrifuged for 5 min at a speed of 5 000 r/min; and B-serum can serve as the protein samples for the positive control of rubber particle aggregation.

几个关键参数为:(1)使用纯化的小橡胶粒子,适宜浓度为在600 nm处的OD值为2.0~2.5;(2)用50 mmol/L Tris-HCl(pH7.4)配制0.5%碱性品红;(3)25 ℃条件下孵育30 min;(4)5 000 r/min离心5 min;(5)B乳清可作为阳性对照的蛋白质样品。

Fusion protein A protein that is made by fusing together two or more different proteins.

融合蛋白通过融合两个或多个不同蛋白产生的蛋白质。

The recombinant protein Pep10 conjugated with GAMP showed strong carrier effect, which provided a novel route for the development of ideal carrier protein.

制备的重组蛋白Pep10与A群脑膜炎球菌多糖偶联能发挥较强的载体效应,为研究理想载体蛋白提供了新的思路。

Coli mainly in a form of inclusion body. The expressed product contained about 25% of total somatic protein and showed good reactogenicity as proved by Western blot. The IgG level against GAMP induced by GAMP-CRM197 conjugate was significantly higher than those by GAMP and by the mixture of CAMP and rCRM197. Repeated immunization with the conjugate induced immunopotentiation, indicating the effect of CRM197 as a carrier protein.

结果重组CRM197在大肠杆菌中主要以包涵体形式表达,表达量占菌体总蛋白的25%左右;Western blot证明重组rCRM197具有良好的反应原性;各针接种后,结合物诱生的多糖特异性IgG水平均显著高于GAMP组和GAMP+rCRM197混合物组,具有较强的免疫原性;多次接种产生了免疫增强效应,重组CRM197具有载体蛋白的作用。

Results Both GAMP derivatives and GAMP-protein conjugates showed GAMP antigen specificity. Compared with GAMP alone, the GAMP-protein conjugates induced high serum anti-GAMP IgG antibody level and strong bactericidal activity in vitro. The conjugate also induced immunological memory.

结果 多糖衍生物、多糖-蛋白质结合物均具有GAMP抗原特异性;结合物免疫小鼠可诱生较单独多糖更高水平的血清抗GAMP IgG抗体和更强的体外杀菌活性,并能产生免疫记忆。

The invention relates to a compound formula for supplement protein, which is mainly made from soy bean protein powder, garden stuff fiber and lecithin.

本发明公开了一种用于补充蛋白质的复合配方,它主要由大豆蛋白粉、果蔬纤维、卵磷脂加制成。

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