查询词典 protein-rich

After fermentation and product purification, we got some purified fusion protein SOD-Thyα1. And the Enterokinase digestion of fusion protein was also studied.

经发酵和纯化得到了融合蛋白SOD-Thyα1的纯品，并对融合蛋白的肠激酶切割特性进行了初步研究。

After the His·Tag fusion protein was cleaved by the enterokinase, intact biologically active PAT was released from the fusion protein and was purified to homogeneity with Ni2+ affinity chromatography .

用肠激酶切除了融合蛋白的融合部分之后再一次通过金属鏊和层析，经过透析后获得了 PAT 纯品。

The expressed IL-29 fusion protein was purified by Ni-NTA affinity chromatography and the fusion tag was removed from IL-29 fusion protein by cleavage with enterokinase.

纯化后的融合蛋白经肠激酶切割和回收后，所得目的蛋白（IL-29）纯度大于96％，该蛋白N-端序列与理论值一致，其抗病毒活性与IFN-α2b相当。

The fused insecticidal protein may delay the process of insects?developing resistance. After investigation of the secondary structure and action mode of Bt insecticidal protein and Cowpea trypsin proteinase inhibitor, as well as the action mechanism of insect enterokinase, we devised the fusion gene on the base of maintaining their functions respectively. The method follows: CpTI gene is linked to 3?-end of GFM crylA gene by a nucleotide sequence encoding cleavage site of insect enterokinase.

根据Bt杀虫蛋白Cry1A和豇豆胰蛋白酶抑制剂的高级结构、杀虫机理及昆虫体内肠激酶的作用机理，本着尽量不影响二者功能的原则，设计出了构建Cry1A和CpTI融合杀虫基因CryCI的方案，即将CpTI基因的编码序列连接到GFM Cry1A基因编码区3'端，并在其间用一段编码肠激酶切割位点的序列相连。

III For constructing the expression vector of a fusion protein and obtain a target protein with full identity on aa sequence of a natural 13- 1,3-1 ,4-glucanase, with the recombined plasmid DNA harbouring the target gene as template, the primers designed with restriction sites for both terminals and enterokinase recognition site, followed by PCR amplification, was induced to the target gene.

为构建融合蛋白表达载体和获得与天然蛋白质序列完全一致的目的蛋白，以含有目的基因的重组质粒DNA为模板，设计引物时加入两端酶切位点及肠激酶裂解位点，通过PCR扩增引入目的基因中，测序结果表明接头和读框正确。

After thermal induction, no specific recombinant protein band in SDS-PAGE was found, but G-CSF activity was detectable. Therefore, a new recombinant plasmid pBVHG2 expressing hG-CSF hybrid protein with additional 8 amino acids which could be cut off specifically with the help of mucosal enterokinase at the N terminus of hG-CSF was constructed.

含此质粒菌株虽然表达菌体裂解后可测得明显的生物学活性，但SDS-PAGE仍未见特异表达产物带；因此，再应用相同方法，在hG-CSF cDNA突变体5′端增加24核苷酸对的FLAG肽编码序列，构建了hG-CSF杂交蛋白（hG-CSF天然蛋白N末端增加8aa的FLAG肽，后者可由肠激肽酶切除）的表达质粒pBVHG2。

Methods: The expression of p16 protein and nm 23 protein were detected by an immunohistochemistory assay in 130 cases of tissue samples including 30 normal ovary, 50 ovarian benign epithelioma and 50 ovarian epithelial carcinoma.

免疫组化SP法检测30例正常卵巢，50例卵巢上皮性良性肿瘤及50例卵巢癌组织中P16蛋白和nm23蛋白，并分析其阳性表达与肿瘤的关系。

Methods: The expression of P16 protein and nm23 protein were detected by streptavidin peroxidase conjugated method in 78 cases of tissue samples including 12 normal ovary,14 ovarian benign epithelioma and 52 OEC.

免疫组化S P法检测12 例正常卵巢，14 例卵巢上皮性良性肿瘤及52 例卵巢癌组织中 P16 蛋白和nm 23 蛋白的表达。

The protein encoded by RBB1 contained two conserve domain ATP-synt-DE-N and AtpC from 3 to 83 and from 1 to 133. All above showed that the full length cDNA RBB1 encoded ATP synthase CF1 epsilon chain protein The plant resistant mechanism is very sophisticated and one of the mainly responses is the metabolic change.

植物的抗病机理非常复杂，其主要反应之一就是代谢上的变化，例如，在利用cDNA微阵列对水稻抗稻瘟病近等基因系的分析中，检测到了很多与光合作用和糖代谢功能有关的差异表达片段；在小麦中也筛选到一个植物中ATP酶具有高度同源性的cDNA序列可能与小麦抗白粉病相关。

The invention further includes pharmaceutical compositions comprising the fusion protein and methods of using the fusion protein and/or the pharmaceutical compositions, for example to stimulate erythropoiesis in subjects in need of therapy.

本发明进一步包括包含所述融合蛋白的药物组合物，和使用所述融合蛋白和/或所述药物组合物(例如在需要治疗的受试者中刺激红细胞生成)的方法。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷