查询词典 protein-rich

With the optimization of the cultivated conditions, the recombinant fusion protein staphylokinase-hirudin was over-expressed in E.coli BL21(DE3), and the final cell density reach 115g wet cell weight per liter, the target protein is about 1.1~1.2g/L accounting for more than 30% of the total protein. The culture process in 5L fermentor was performed on 40L fermentor successfully, and indicated that the process was easy scalable.

通过对培养条件的不断优化，重组葡激酶-水蛭素融合蛋白在大肠杆菌BL21(DE3)里得到了高效表达，菌体密度最终达到115g/L以上，可溶性重组融合蛋白占菌体总蛋白的30%以上，含量约为1.1~1.2g/L.5L发酵罐的发酵工艺参数在40L发酵罐中进行了放大培养，结果表明该工艺能有效的放大，可适用于工业生产。

In this research, the spider silk from the major ampullate gland of native Nephila pilipes was collected via the forced-silking apparatus at the reeling speed of 3 m/min. The obtained silk was dissolved in hexafluoro-isopropanol. The influence of pH and temperature over the secondary structure of the silk protein in solution state was examined via Circular Dichroism. The effect of silk concentration and electrical field strength on the structure conformation of silk protein during film casting process was investigated via FT-IR. During the drying process, the increase of silk concentration and the presence of electric field tend to form β-sheet and β-turn. Finally, the dried film of silk protein was treated with different temperatures and cation solutions to study the post-treatment effects.

本研究所采用之蜘蛛丝蛋白质系取自本土品种人面蜘蛛之大囊壶腺体，以3 m/min之卷丝速度强迫取丝(forced-silking)收集蜘蛛丝，再将纤维溶於六氟异丙醇溶剂中配制成溶液，以圆二色光谱仪分析在不同 pH 值与温度条件下丝蛋白质在溶液中之二级结构变化，再将蜘蛛丝溶液以不同浓度及电场强度下乾燥成薄膜后，以傅立叶红外线光谱仪检测分析丝蛋白质在固体状态下之二级结构，最后以不同温度加温、离子溶液浸泡等条件对乾燥所得之薄膜进行后处理，并分析其二级结构之变化。

Patients who are sensitive to protein,whose own body sensitive,due to one-time edibling a great deal of food that contains protein,such as egg,which is easy to suffer protein sensitive urticaria,diarrhea,bellyache,skin anthema and so on.

对蛋白质过敏一些本身体质过敏的病人，由于一次性摄人大量的含蛋白质食品，如蛋类等，易发生蛋白过敏性荨麻疹，出现腹泻、腹痛、皮肤出疹等现象。

AVI may be protein matrix and it possesses neither a membrane boundary nor an internal structure,its formation is the result of the anthocyanins transported into the vacuole bind with a protein matrix. In vacuole, AVI is irregular and jelly-like in shape. In AVIs, the attachment of anthocyanins to the matrix protein is likely to be via H-bonds to a sterically restricted site. AVI is suggested to act as vacuolar anthocyanin "trap", preferentially for anthocyanidin 3, 5-diglycosides or acylted anthocyanins. The emergence of AVI can enhance color intensity and results in the "blueness" of color in the vacuole.

花色苷液泡包涵体可能具备蛋白质基质，既无膜包裹又无内部结构，其形成是转运进液泡的花色苷与蛋白质基质结合的结果；液泡里的花色苷液泡包涵体形状不规则，象果冻；在花色苷液泡包涵体中，花色苷可能通过氢键连接于蛋白质基质的一个有限空间位点；花色苷液泡包涵体被认为是液泡中花色苷的&陷阱&，优先摄取花色素3，5-二糖苷或酰化的花色苷；花色苷液泡包涵体的存在可增加液泡色彩的强度并导致&蓝化&。

In the process of root cutting, the root accumulated a lot of soluble sugar and soluble protein, which provided material and energy for the cell differentiation. As root differentiating, soluable protein was continuously used up. During 10 to 20 days, the content of soluble sugar and soluble protein in the basipetal top and end of rootkept comparatively stable, then declined as adventitious buds and roots growing. 40 days later, they kept invariable, because the newly formed bud can photosynthesize and the newly formed root can absorb nutrient from outside. The dynamic balances of endogenous hormone regulate adventitious buds and roots of the root. During callus growing and adventitious bud differentiating.

上部根段在细胞分化前期积累了大量的可溶性糖，为后期的分化提供能源物质，同时积累了大量的可溶性蛋白质，为愈伤组织的产生提供能源，而后随着分化，可溶性蛋白质不断被消耗。10～20d根段上部及下部的可溶性糖、可溶性蛋白质含量保持在相对稳定的水平变化不大，后期随着不定芽及不定根的生长下降较快。40d后，新形成的芽已经能够进行光合作用，新形成的根也可从外界吸收营养物质，可溶性糖、可溶性蛋白质含量基本保持不变。

Results showed:(1) The airway responsiveness of smoke control group was higher than that of normal control group, the airway responsiveness of smoke + exercise group was lower than that of smoke control group;(2) Plasma level of cortisol determined immediately after exercise was higher than that determined before exercise, no significant difference occurs between that determined next day morning after all exercise finished in contrast to that determined before exercise;(3) HE staining showed, there was severe chronic pulmonary inflammatory response in smoke control group, which was reduced in the smoke + exercise group;(4) chronic cigarette smoke downregulated the protein and mRNA expression of BKca in smooth muscles of bronchi and bronchioli, exercise increased the mRNA expression of BKca;(5) chronic cigarette smoke downregulated the protein and mRNA expression of Kv1. 5 in smooth muscles of bronchi and bronchioli, exercise increased both the protein and mRNA expression of Kv1. 5 in contrast to smoke control group in bronchioli and did not effect the expression of Kv1. 5 in bronchi;(6) chronic cigarette smoke induced upregulation of FIZZ1/RELMα in bronchial smooth muscle cells, exercise decreased these effect.

结果：(1)吸烟对照组的气道反应性明显增高，吸烟加运动应激可明显降低大鼠的气道反应性；(2)吸烟运动组运动后血浆皮质醇浓度明显高于运动前，但全部运动结束后次日晨测定的血浆皮质醇浓度与运动前无明显差异；(3)HE染色显示，吸烟对照组肺组织出现明显的慢性炎症反应，吸烟加运动应激组比吸烟对照组炎症反应轻；(4)慢性吸烟可降低大鼠大气道和小气道BKca mRNA和蛋白表达，而吸烟加运动应激可使BKca mRNA表达上调，但对小气道的蛋白表达无影响；(5)慢性吸烟可降低大鼠大气道和小气道Kv1.5蛋白和mRNA表达，在小气道吸烟加运动应激可减轻这种作用，大气道则无作用；(6)吸烟使支气管平滑肌的FIZZ1/RELMα蛋白表达明显增强，而吸烟加运动组的RELMα蛋白及mRNA均明显减弱。

Cloned one virus binding protein from Rhopalosiphum maize Yangling biotype through PCR technique, using designed specific primers based on this protein partially sequence. In nucleotide and amino acid sequenece levels, this protein was identified as GroEL protem of endosymbiotic bacterium (Buchnera sp.) of aphid.

以玉米蚜杨凌生物型为材料，设计特异性引物采用PCR的方法在国内首先克隆了一种玉米蚜体内参与传毒的共生菌groEL基因，序列测定结果表明：玉米蚜杨凌生物型共生菌groEL基因全长为1647bp。

However, dexamethasooe did not seem to affect the sustained current and the sustained component of the biphasic current induced by ATP. The in hibitory effect of dexamethasonc on ATP induced currents was blocked by glucocorticoid receptor antagonist RU38486 (10μmol/L) and protein kinase A inhibitor H-89 (10μmol/L), but not by G protein inhibitor GDP-β-S (0.2mmol/L) and protein kinase C inhibitor chelerythrine chloride (10μmol/L).

地塞米松对ATP激活电流的快速抑制作用可被糖皮质激素受体拮抗剂RU38486(10μmol/L)、蛋白激酶A抑制剂H-89(10μmol/L)阻断，而G蛋白激活抑制剂GDP-β-S(0.2mmol/L)、蛋白激酶C抑制剂Chelerythrine chloride(10μmol/L)对地塞米松的快速抑制作用无明显影响。

Objective To study the expression of monocyte chemotactic protein-1(MCP-1)mRNA and protein induced by advanced oxidation protein productsin smooth muscle cells.

目的 研究晚期氧化蛋白产物对血管平滑肌细胞单核细胞趋化蛋白-1表达的影响。

Results The levels of HSP 70 mRNA and HSP 70 protein expression were significantly different among the 5 groups. Ketamine induced marked HSP 70 mRNA and HSP 70 protein expression in the posterior cingulated cortex. Propofol itself did not induce HSP 70 gene expression in this brain region. Propofol significantly inhibited ketamine-induced HSP 70 mRNA and HSP 70 protein expression in the posterior cingulate cortex in a dose-dependent manner.

结果氯胺酮可明显诱导HSP70 mRNA与HSP70蛋白在大鼠后扣带回皮质区的表达；异丙酚自身不能诱导HSP70基因的表达；预先给予异丙酚可显著抑制氯胺酮诱导的HSP70 mRNA和HSP70蛋白在这一区域的表达，且抑制效应呈剂量依赖性。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。