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The EBA of protein with the anion exchanger Streamline DEAE was examined by investigating the effect of liquid flow rate, liquid viscosity and protein concentration. A modified mathematical model was established to describe protein adsorption behavior in the expanded bed chromatography by taking into account the axial size classification of the adsorbent within the bed.

研究了阴离子交换吸附剂 Streamline DEAE 的蛋白质吸附特性及其膨胀床的蛋白质动态吸附行为,考察了液相粘度、流速和蛋白质浓度对色谱穿透曲线的影响。

The results showed that:(1) The relative weight gain, special growth rate, feed conversion rate, feed protein efficiency and protein deposition rate were improved significantly (P.05) by supplementing compound acidifier in common carp diet.(2) The protease activities in the liver pancreas, fore and middle intestines increased significantly (P.05) by supplementing 0.3% compound acidifier. The activities of amylase and lipase were improved after the supplementation, but not at significant level.(3) Supplementation of compound acidifier in common carp diet did not significantly affect the fish body protein, lipid, ash, moisture contents, coefficient of fatness and viscerosomatic index.

结果表明:1添加复合酸化剂显著(P.05)提高鲤鱼的相对增重率、特定生长率、饵料转化效率、饲料蛋白质效率以及饲料蛋白质沉积率等生长性能指标;2添加质量分数0.3%的复合酸化剂时,鲤鱼肝胰脏、前肠和中肠的蛋白酶活性显著提高(P.05),酸化剂组的淀粉酶和脂肪酶活性有升高的趋势,但与对照组差异不显著;3鱼体的粗蛋白质、粗脂肪、粗灰分、水分、肥满度及内脏比等品质指标在各组间差异不显著。

Bioenergy transfer appears in the motor-protein, actin, actomyosin, collagen-protein and so forth, so thatα-helical protein with three-chain becomes the object of study.

由于生物能量传递是在马达蛋白,肌动蛋白以及胶原蛋白等中进行的。

The preliminary structure analyzing indicated that this protein was a hydrophilic protein; comparised of Batrachuperus pachunii β-microseminoprotein precursor-like protein sequence to other speices, 10 cystenies and other 15 aminooacids were conserved and located at the same positions as those in amniote β-microseminoprotein.

初级结构分析表明,该蛋白属于亲水性蛋白;多重序列比较显示,其氨基酸序列中的10个半胱氨酸位点及15个其他氨基酸位点与高等脊椎动物β-microseminoprotein中同种氨基酸有相同的位点。

The yolk proteins were collected through the anatomization of the maiden eggs and the eggs in different embryo development stages. The yolk proteins were separated by two-dimensional electrophoresis and followed the image analysis. Over 203 protein spots were found to exist in the yolk protein of silkworm eggs. Some different patterns of yolk protein spots were found in silkworm of various varieties. While, little variation of yolk proteins was detected during different development stages of any silkworm variety.

采用直接解剖蚕卵获取卵黄蛋白,结合蛋白质双向电泳和图像分析,对家蚕处女蛾卵及不同胚胎发育阶段蚕卵的卵黄蛋白质进行了研究,发现家蚕卵黄蛋白有 203 个蛋白质斑点;不同家蚕品种之间卵黄蛋白的组成存在一些差异;同一品种不同发育时期胚胎卵黄蛋白的组成基本保持不变。

Finally, the fusion protein was expressed in E coli DH5α under induction by arabinose. And the fusion protein was 65 kD and account for 30% of the total protein of the bacteria.

SDS-PAGE电泳分析显示,融合蛋白分子量约65 kD,与预期蛋白分子量一致,并且目的蛋白占菌体蛋白的30%。

To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中,苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变,并筛选在自生生长时期、共生生长时期的表型,以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因,我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法,发现lsrA基因主要在根瘤侵染区开始表达,表达时序也在侵染阶段左右,但晚于bacA基因表达;LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体,nifA和fixK基因的转录水平降低,nifH基因的转录被完全阻断,因此LsrA蛋白为根瘤发育所必需,是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因,并研究了它的调节特性。oxyR突变后,苜蓿中华根瘤菌对过氧化氢敏感性提高,适应性降低。

Superscript 15N tracing results suggested that the protein in "201" rice straw could be effectively transformed into fish body protein and mice body protein, The digestibility of fodder, the (superscript 15)N recovery rate of animal body and the absorption of fodder protein were 13.8%, 9.6%, 24.49% and 16.5%, 6.0%, 47.2% higher than those of common rice straw respectively. When grass carp and mice were fed with "201" rice straw, the (superscript 15)N recovery rates of animal manure were 3.25%, 6.5% lower than those of common rice straw, respectively.

上标 15N示踪结果表明,&201&稻草中的蛋白质能够有效地转化为鱼体蛋白和小白鼠的体蛋白;&201&稻草饲养草鱼和小白鼠的饲料消化率、动物体(上标 15)N回收率、动物吸收饲料蛋白分别比一般稻草高13.8%、9.6%、24.49%和16.5%、6.0%、47.2%,而动物粪便的(上标 15)N回收率却比一般稻草低3.25%和6.5%。

The prediction equations of nutritional requirements about digestible energy, metabolizable energy, digestible crude protein, and protein deposited for stall-feeding growing crossbred Boer goats were:DE, kJ/d=588.31W0.75 9.25W 879.9 r=0.5712, p=0.013ME, kJ/d=482.42W0.75 7.57AW 721.57 r=0.5713, p=0.013DCP, g/d=(0.03AW 1.27) W0.75 r=0.9869, p=0.0131 PD, g/d=(0.03W 0.03) W0.75 r=0.9993, p=0.0007 When the average daily gain stage is from 0 to 200g/d,for the stall-feeding growing crossbred Boer goats, aged at 3~6 months, Metabolic rate of gross energy of the diets is 0.55; digestibi I ity of crude protein is 0.56; The protein requirement of per live weight gain for meat goats is 0.31 g PD/g gain .

舍饲波杂肉羊生长期消化能、代谢能、可消化粗蛋白质、沉积蛋白质需要量的估测模型分别为: DE,kJ/d=588.31W~(0.75) 9.25△W 879.9 r=0.5712,p=0.013 ME,kJ/d=482.42W~(0.75) 7.57△W 721.57 r=0.5713,p=0.013 DCP,g/d=(0.03△W 1.27)W~(0.75) r=0.9869,p=0.0131 PD,g/d=(0.03△W 0.03)W~(0.75) r=0.9993,p=0.0007 3~6月龄舍饲生长期波杂肉羊增重范围为0~200g/d时,日粮总能代谢率平均为0.55,粗蛋白质的消化率平均为0.56;每增重1g体沉积蛋白质需要量为0.31g。

The aim of the USN network is to clarify the function and structure of certain protein complexes, while the remit of the SMP Protein project is to investigate disease-related protein-protein interactions on a molecular basis.

超微结构网研究计划的目标就是彻底了解确定蛋白质复合体的功能和结构,而系统方法平台蛋白质计划的目标则是在分子基础上对与疾病相关的蛋白质与蛋白质相互作用进行研究。

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