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protein-rich相关的网络例句

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Specific protein bands were observed in cellular and extracellular protein electrophoresis for both strains 593 and 18.There were also different protein expression in certain protein bands.It is indicated that a significant difference existed between the proteome of strains 593 and 18.This difference may contribute to the different cariogenic potential of these two strains.

两菌株的菌体和菌外可溶性蛋白电泳图谱中均可见各自特异表达的蛋白条带,且部分相同位置的蛋白条带在蛋白表达量上也存在差异,表明两菌株的蛋白组存在明显差异,该差异可能是导致其致龋力差异的原因之一。

At concentration of 2% PEG8000, 30% microsomal protein containing 10% of total P450 was precipitated. At concentration of 5% PEG8000, 55% microsomal protein containing 20% of total P450 was precipitated. At concentration of 8% PEG8000, 78% microsomal protein containing 30% of total P450 was precipitated. At concentration of 11% PEG8000, 85% microsomal protein containing 70% of total P450 was precipitated.

当PEG的浓度为2%时,约30%的微粒体蛋白和10%的P450被沉淀;PEG为5%时,约55%的微粒体蛋白和20%的P450被沉淀;PEG为8%时,约78%的微粒体蛋白和30%的P450被沉淀;PEG为11%时,约85%的微粒体蛋白和70%的P450被沉淀。

The deleted mutant PAP gene was also cloned into yeast secreted expression pPIC9K vector to form pPIC9K~3, then the vector was transferred into Pachia pastoris GS115 strain. The specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50-60 U g per millilitre measured by UV-absorbed methods in the supernatant of the medium via high density fermentation. SDS-PAGE results showed that there was one protein band in the gel which molecular weight was about 34Ku . The protein could specific react with France PAP antiserum in Western-blotting. Activity tests revealed t

将缺失型PAP基因克隆于酵母分泌型表达载体PPICgK构成重组载体,然后导入毕赤酵母(P8chia nastoris)菌株GSlls细胞中,在甲醇的诱导下,经过酵母高密度发酵进行PAP的表达,经SDS-PAGE分析,结果表明,在培养基上清液中含有一明显的特异性蛋臼条带,大小为34Ku,经Western-blotting分析,该蛋白与法国PAP抗血清有特异性反应,体外活性检测表明该蛋白对TMV的侵染性具有高度的抑制性,说明该 PAP基因在毕赤酵母 GS中也得到了正确表达。

Woodchucks . The HBV genome has four genes: pol, env, pre-core and X that respectively encode the viral DNA-polymerase, envelope protein, pre-core protein (which is processed to viral capsid) and protein X. The function of protein X is not clear but it may be involved in the activation of host cell genes and the development of cancer.

乙型肝炎病毒基因组有四个基因:波兰,环境,前核心和X ,分别编码病毒DNA聚合酶,包膜蛋白,前核心蛋白和蛋白质十大功能蛋白X是尚不清楚,但它可能参与激活宿主细胞的基因和癌的发展。

And objective parameters (texturized degree, color, hardness, springiness, chewing, water absorption, yield, etc.) were investigated in the high moisture soy protein extrusion process, and the statistical models of system and objective parameters were built, using the step-by-step regression analysis; A comprehensive evaluation of the products, and the process optimization of high moisture extrusion were obtained,using factor analysis; Secondly, residence time distribution and soy isoflavones losses kinetics were studied in the high moisture extrusion; Finally, chemical bonding process, the micro-structure and protein secondary structure changes were investigated in the texturization of soy protein, then the mechanism assumptions of texturization soy protein by moisture extrusion were proposed.

本文对大豆蛋白高水分挤压组织化技术和机理进行了研究,研究内容包括:首先采用系统分析法,研究了大豆蛋白高水分挤压过程中,挤压机操作参数(螺杆转速、物料水分、喂料速度和机筒温度)对系统参数(系统压力、扭矩、单位机械能等)、目标参数(组织化度、色泽、硬度、弹性、咀嚼性、吸水率、产量等)的影响规律,建立了各因变参数的统计模型;采用因子分析法对产品进行了综合评价,并对高水分挤压组织化工艺进行了优化;其次,研究了大豆蛋白高水分挤压中的停留时间分布以及大豆异黄酮的损失动力学;最后,研究了大豆蛋白组织化过程中化学键、微观结构和蛋白质二级结构的变化规律,提出了大豆蛋白高水分挤压组织化的机理假设。

The protein interaction domains was delineated at the N-terminal 50-amino-acid fragment of HCV core protein and the C-terminus of p53. Confocal analysis also revealed that these two proteins colocalize in subnuclear granules and peri-nuclear region. Transfection experiments using a p53-responsive reporter plasmid in HuH-7, Hep3B, HepG2 and H1299 demostrated that full-length HCV core protein could elicit a positive or negative effect on the p53-mediated transcriptional activation depending on the concentration of the HCV core protein.

更且利用p53蛋白C端删除55-95个胺基酸之质体与含p53蛋白结合区之报导基因和表现全长HCV核心蛋白三者之质体於H1299细胞株进行共同转染时,与全长p53蛋白比较,发现HCV核心从增强全长p53蛋白之转活化能力转为抑制p53蛋白缺乏C端55-95个胺基酸之转活化能力,因实验室已有结果证实活体外及活体内缺乏C端55个及75个胺基酸之p53蛋白无法与HCV核心蛋白进行结合(Kao, unpublished data)。

Because protein synthesis costs energy while protein canalso be used as a source of available energy,there is an interaction during growthbetween the utilization of dietary protein-free energy and protein.

在自由采食量的预测方面,提出了用累计采食能量对时间进行多项式拟合的方法。该方法可以避免预测自由采食量时的系统偏差,并可应用到对模型的调试和验证中去。

Results Well-resolved,reproducible 2-DE patterns of SGC7901/VCR and SGC7901 were established.Silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.Gels stained with colloidal Coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.

结果 获得了背景清晰、重复性好的双向凝胶电泳图谱,两种染色凝胶相比,硝酸银染色在样品少时显示更佳,过量则影响图像质量,而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加,并无明显拖尾。

Results: In cellular soluble protein electrophoresis,specific band about 75 KDa was observed in 593 strain.The differences of protein expression at 85,60,50,40KDa between 593 strain and 18 strain were observed.In extracellular soluble protein electrophoresis,specific bands at 75 KDa and 40 KDa were observed in 593 strain;specific band at 50 KDa was observed in 18 strain.The difference of protein expression amount in about 60KDa between 593 strain and 18 strain was observed.

结果:菌体可溶性蛋白电泳图中见593号菌株在75 KDa左右存在特异表达的蛋白条带,且二者在85、60、50、40 KDa左右蛋白表达量上存在差异;菌外可溶性蛋白电泳图中见593号菌株在75、40 KDa左右有特异表达的蛋白条带,而18号菌株在50 KDa左右有特异表达的蛋白条带,二者在60 KDa左右蛋白表达量上存在差异。

The authors determined glutenin protein,soluble in acid glutenin protein, surplus protein content in 21 species of wheat and indexes of rheological characteristics and made sedimentation and bread baking test. By using stepwise regression and path analysis methods,it was further proved that prediction of bread baking quality of wheat by Surplus protein content is more precise than that by sedimentaion and approximately equal to that by the score of flour. So the authors suggest that the direct selection of ...

本试验测定了21个小麦品种的几种蛋白含量以及流变学特性指标,作了沉淀试验和面包烘烤试验,借助于逐步回归分析和通径分析方法,进一步验证了用剩余蛋白含量预测小麦烘烤品质与评价值相当,比沉淀值准确的结论,并建议在小麦品质育种及面包烘烤品质分析中,加强对剩余蛋白含量的直接选择。

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