查询词典 protein-rich

Protein in breast milk has 27%α-lactalbumin, and milk α-lactalbumin protein only full 4%, a-whey protein to provide amino acids closest to breast milk composition, protein enhance biological utilization, lower total protein, so as to effectively reduce the burden on the kidneys.

母乳中的蛋白质有27%是α-乳清蛋白，而牛奶中的α-乳清蛋白仅占全部蛋白质的4%，a-乳清蛋白能提供最接近母乳的氨基酸组合，提高蛋白质的生物利用度，降低蛋白质总量，从而有效减轻肾脏负担。

The result showed that : soybean protein and corn protein which were primarily improving the capability of mulsification were selected as the appropriate protein material for protein bar, 10% of saccharose was appended and 20% of moisture was adjusted, perfect protein bar was available by extrusion .

结果表明采用乳化性大豆蛋白和乳化性玉米蛋白为蛋白原料，添加10%的蔗糖和调整含水量为20%，利用挤压膨化可得到较为理想的蛋白棒。

The persent of each component protein can be measured :total protein 52.72 per, albumin 14.36 per, globin 9.30per, titanium 12.87 per , alcoholy protein 2.29per, alkalize protein 2.62per, hawless protein 6.08per.

结果发现：脱脂蝇蛆粉的总蛋白质含量为52.72%，清蛋白14.36%，球蛋白9.30%，小分子蛋白12.87%，醇溶蛋白2.29%，碱溶蛋白2.62%，难溶蛋白6.08%。

The p53 gene structural abnormality and mdm2 protein overexpression might be two factors causing p53 protein overexpresion in NPC. The function of p53 proteins produced by various structural anormality of p53 gene might be different in activating MDM2. The overexpression of p53 or mdm2 protein, particularly both cooperation, might play an important role during NPC genesis.Key words: Nasopharyngeal neoplasms; p53 gene; p53 protein; mdm2 protein

p53基因结构改变和mdm2蛋白过度表达是影响NPCp53蛋白过度表达的两个因素；不同类型p53基因结构改变所产生的p53蛋白激活MDM2的功能并不相同；p53和mdm2蛋白过度表达，尤其两蛋白协同作用，可能在NPC发生中起重要作用。

With the development of molecular biology, based on yeast two-hybrid, many techniques such as yeast one-hybrid, reverse two-hybrid, three-hybrid, and extranuclear two-hybrid were set up to be applied in the research on the interaction between protein-protein, protein-DNA, protein-RNA, and protein-ligand.

随着分子生物学的发展，在酵母双杂交的基础上又建立起了酵母单杂、反向双杂交、三杂交及核外双杂交等多项技术，并已经成功地运用于蛋白质之间、蛋白质与DNA、RNA和配体之间相互作用的研究。

Because of no signal from N-terminal protein sequencing for 43kDa protein directly, a resulting 18kDa peptide from the partial cleavage of 43kDa protein by BrCN had its N-terminal sequences of AFTFKK determined by N-terminal protein sequencing assay. Then, the 43kDa protein was convinced to the enzyme of 3phosphate glycerate kinase (PGK1) by searching YPD.

由于43kDa蛋白直接进行蛋白质氨基端序列测定时没有信号，因而用溴化氰部分化学裂解43kDa蛋白，得到的18 kDa多肽经蛋白质氨基端序列测定，得到其氨基端前6个氨基酸残基序列为AFTFKK，通过计算机检索YPD，确定43 kDa蛋白是3—磷酸甘油酸激酶（PGK1）。

In the second trial, five groups of diets with isonitrigenous (40% protein) and isoenergetic 20 MIJg^(-1 gross energy and different relativity of essential amino acid balance with soybean protein replacing 0.0%, 13.5%, 27.0%, 40.5% and 54% of fish meal protein, were formulated to determine optimal replacement level of soybean protein to fish meal protein.

实验Ⅰ以褐鱼粉为蛋白源，配制5个蛋白水平(31.04%、35.51%、40.89%、46.62%、50.33%)的等能、等必需氨基酸平衡关联度的半精制饲料，探讨翘嘴红鲌对饲料蛋白的需求；经过8周饲养，实验Ⅰ的结果表明，饲料蛋白含量对翘嘴红鲌的增重率、饲料效率和蛋白效率具有显著影响。

Egr-1 mRNA and Egr-1 protein hadn't been found in the normal vein. The expressions of Egr-1 mRNA and Egr-1 protein had biphasic changes. By reverse transcription-PCR and in situ hybridization, we found that the level of Egr-1 mRNA rose at 1 hour after graft, the expression of Egr-1 mRNA was (35±7)%. Decline at hour 6, 24 and day 3, the positive rates of Egr-1 mRNA were (8±2)%,(8±6)% and (8±4)% respectively. Reincrease at day 7, a peak at day 28, the positive rate of Egr-1 mRNA was (45±6)%(compared with other phase, P＜0.01). At day 42, the expression of Egr-1 mRNA declined again. Immunohistochemical staining and Western blot revealed Egr-1 protein had expressed at hour 2 early phase, the expression of Egr-1 protein was (30±5)%, and until to hour 6. The level of Egr-1 protein was decrease at hour 24 and day 3, the positive rates were (7±3)% and (7±8)% respectively.

结果 自体静脉移植后，Egr-1 mRNA和Egr-1蛋白的表达呈双相变化，即移植后1 h， Egr-1 mRNA表达迅速升高，阳性率为（35±7）%，6 h、24 h及3 d时下降到较低水平，阳性率分别为（8±2）%、（8±6）%和（8±4）％，7 d时又再升高，28 d时达高峰，阳性率为（45±6）%，此与其余各时点比较差异均有统计学意义（P＜0.01），42 d时，Egr-1 mRNA的表达再次下降；移植早期（2 h）即有Egr-1蛋白的表达，阳性率为（30±5）%，并持续至6 h，24 h～3 d表达下降到较低水平，阳性率分别为（7±3）%和（7±8）%，7 d时又再升高，至移植后28 d，Egr-1蛋白的表达阳性率达到高峰，为（40±9）%，此与其余各时点比较差异有统计学意义（P＜0.01）。

①The collected clinical Acinetobacter baumannii isolates were more seriously resistable to Ciprofloxacin than to Imipenem;②The antibiotic resistance of clinical Acinetobacter baumannii isolates may be associated with the decline of CarO protein expression;③There is some relationship between CarO protein and ciprofloxacin-resistant Acinetobacter baumannii strains,it proves that CarO protein supports bacteria on multi-drug resistance;④CarO protein may be related to cell signalling;⑤The difference in CarO protein functional sites and structure between the sensitive strains and the resistant strains may be related to bacterial resistance.

①收集的临床鲍曼不动杆菌对环丙沙星的耐药情况较亚胺培南严重；②CarO蛋白的表达下调可能与临床鲍曼不动杆菌的耐药性相关；③CarO蛋白与鲍曼不动杆菌耐环丙沙星有一定相关性，佐证了CarO蛋白可能与细菌多重耐药有关；④CarO蛋白可能与细胞信号传导有关；⑤敏感菌株与耐药菌株的CarO蛋白功能位点和内部结构有差异，可能与细菌耐药有关。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐