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Pokeweed antiviral protein, a 29-kD protein isolated from Phytolacca americana, inhibits translation by catalytically removing a specific adenine residue from the large rRNA of the 60s subunit of eukaryotic ribosomes. In addition to its ribosome-inactivating ability, PAP has potent antiviral activity against many plants and animal viruses.

美洲商陆抗病毒蛋白(pokeweed antiviral proteins,PAPs),是在美洲商陆的不同组织或不同生长阶段生成的一类具功能的单链核糖体失活蛋白(ribosome-inactivating proteins,RIP),是天然的广谱抗病毒生物活性物资,能抗多种植物和动物病毒。

The PILE protein was successfully purified and initially established a ELISA detection methods for the diagnosis of Legionnaires disease which used the type Ⅳ pilin protein PILE as the coating antigen.

成功纯化了Ⅳ型菌毛蛋白PILE,初步建立了一种以Ⅳ型菌毛蛋白PILE为包被抗原的诊断嗜肺军团菌病的ELISA检测方法。

Methods The type Ⅳ pilin protein PILE was purified by HisTrapTM HP affinity columns ,then the purified protein was examined by SDS-PAGE and Western blot.

使用HisTrapTM HP亲和层析柱纯化Ⅳ型菌毛蛋白PILE,并对纯化蛋白进行SDS-PAGE和Westernblot鉴定。

The plastein reaction is considered to involve the formation of polypeptides by a reversal of the usual peptide bond hydrolysis to produce "resynthesized" protein or polypeptides from a hydrolysate of protein.

类蛋白反应通常认为是酶水解的逆反应,是指在一定条件下,通过蛋白酶的催化作用将高浓度的蛋白质水解物与蛋白酶一起加热处理,生成一种类似高分子蛋白质物质的反应。

Fifty-two protein peaks in the sera were significantly different between 57 patients with tuberculous pleuritis and 70 controls(P<0.01).The protein peak at m/z 5643 was highly expressed in 47.4%(27/57) sera from the patients with tuberculous pleuritis,and lowly expressed in 4 sera from the patients with non-TB pleuritis.

因此,选择5643 m/z蛋白作为潜在的诊断标志物,经RP-HPLC分离、纯化,并利用MALDI-TOF-MS及LC-MS/MS进行鉴定,5643 m/z目标蛋白质是粘蛋白,又称为α-1-酸性糖蛋白。57例结核性胸膜炎患者血清和70例对照血清蛋白指纹图谱比较,有52个差异蛋白峰(p<0.01)。

The contents of chlorophyll were also decreased. New protein spots were induced and some protein spots were lost in root, plumular axis, chloroplast, when seedlings were treated with 1.0 mmol/L Cd(superscript 2+). A positive relationship was observed between the inhibition of seedling growth and changes in biochemical characteristics.

在1.0mmol/L Cd(上标 2+)溶液处理下,与对照相比,黄瓜幼苗的根、胚轴、叶绿体中的蛋白质组分发生变化,即蛋白缺失、新蛋白合成以及蛋白表达量的上调或下降,同时其生化性状(蛋白质组分、叶绿素含量)的变化与幼苗生长抑制呈正相关。

After induced with IPTG, the proteins expressed by different fragments were analyzed by SDS-PAGE, and the results showed that all these truncated proteins could be expressed in E. coli BL21. Bioassay result against Plutella xylotella showed that the active fragment of Cry1Ba3 protein was located between amino acid No. 22 and No. 655. As the insecticidal activity of the truncated protein containing amino acid No. 54-655 was declined significantly, and No. 22-627 and No.

采用浸叶法检测这些蛋白片段对小菜蛾的杀虫活性,研究结果表明含有第1~685位和含有第22~655位氨基酸的蛋白片段对小菜蛾的毒性,与全长Cry1Ba3蛋白相比,没有改变;含有第54~655位氨基酸的蛋白片段对小菜蛾的毒力明显降低;而含有第22~627位和含有第85~655位氨基酸的蛋白片段完全丧失了对小菜蛾的活性。

Total protein content in serum maintained the highest level (P<0.10) and urea concentration in serum maintained the lowest level (P<0.10) similar to the condition in primiparous gilts which might mean higher protein efficiency to maternal body and fetus.

2经产妊娠母猪血清雌激素浓度在妊娠期维持相对高水平;孕激素妊娠前期维持相对高水平,后期维持相对低水平,其繁殖性能较好,与初产母猪的情况一致。

Calpain system is probably the major proteolytic in protein degradation,which plays an important role in myofibrillar protein degradation.

近年的研究表明,calpain是细胞质中主要的蛋白水解酶,在肌原纤维蛋白降解中起着重要的作用。

The ASK1 (ARABIDOPSIS SKP1-LIKE) protein is a critical component of the SCF (Skp1-Cullin-F box protein) ubiquitin ligase complexes that recruit target proteins for degradation by the 26S proteosome.

这些研究丰富了人们对nsLTP基因功能的认识,为进一步克隆其受体基因并最终阐明nsLTP介导的信号传导途径奠定了基础。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?