查询词典 protein-free

The most of the product became soluble from inclusion body by lowering induction temperature. At the same time the gene was cloned into vector pMAL-p2X, which was translated in E. coli TB1 and expressed a fusion protein. We optimized the induction time and achieved high expression. But there was no obvious product secreted in the periplasm extract. Almost all fusion protein is expressed soluble in cytoplasm.

利用融合型表达载体pTYB11-EFE融合型表达，对诱导剂IPTG的浓度和诱导温度进行了优化，通过降低诱导温度使产物由包含体变成可溶性融合型产物；利用融合型分泌表达载体pMAL-p2X构建了表达载体pMAL-p2X-EFE-7~#，对诱导时间进行了优化，获得了较高的表达量，但发现产物没有明显的分泌表达，绝大部分是以可溶形式存在于胞内。

The structure and form of peritrophic membraneof Helicoverpa armigera were observed by electron microscopy.The type of protein,the content of protein and carbohydrate in PM were analyzed.

应用电子显微技术和生化技术，研究了棉铃虫Helicoverpaarmigera围食膜的超微结构和表面形态，初步分析了围食膜蛋白质的种类，测定了蛋白质和糖的含量。

However, the Cry1Ac-GFP fusion protein only accreted with peritrophic membrane of resistant strain and showed fluorescence, which suggested that the binding of peritrophic membrane with Cry1Ac toxin protein may result in resistance of larvae of H. armigera to Cry1Ac insecticidal crystal proteins.

比较Cry1Ac杀虫蛋白敏感和抗性棉铃虫幼虫中肠的发光部位，敏感棉铃虫幼虫的中肠围食膜已经消失，肠壁细胞发出强烈的荧光，而抗性棉铃虫的围食膜较健全并发出荧光。

In order to explain the toxic mechanism, it studied the toxicity effect of each chemical for Chl-a and protein and finds that chlorobenzene has the most toxicity to Chl-a and protein, bringing much phaeophytin and effecting photosynthesis efficiency.

探讨了六种化学品对小球藻的致毒机理，发现氯苯对小球藻的叶绿素a含量及蛋白质含量的影响最严重，并且在化学品的胁迫作用下产生大量的脱镁叶绿素a，影响藻类的光合作用效率。

To facilitate the applications of biologically functional protein in drug design, it is necessary to have a thorough understanding of their conformations and their interactions with the receptors. NMR spectroscopy has the unique ability to retrieve information about 3D structures of proteins and their interactions with the target proteins in solution. Many NMR techniques have been used to study a number of pharmaceutically important proteins and characterize their interactions with the targets. In this review, we will introduce these NMR methods and show how to utilize them for protein drug design.

为了设计具有生物功能性的蛋白质来当作药物，我们必须对其蛋白质结构与其受体在结合时的作用有完整清楚的了解，而核磁共振光谱具有独特的能力，可以获得溶液状态下蛋白质结构以及蛋白质与标的蛋白质交互作用间的资讯，目前已有许多核磁共振光谱技术运用到蛋白质药物的研究，并确认它们与其标的蛋白质间的作用模式，笔者将在本文中介绍这些技术，以及如何运用它们来获取重要资讯，俾设计蛋白质药物。

In the scorch stage, average yield of withered grass of second and third year growth prematurity type of Phleum pratense L. is 10672.5 kg/hm2, by cradling one time per year, average yield of wind hay grass is 7575 kg/hm2, the plant average height of selective colony is 160.0cm, the plant height of colony is rather equality. In the course of general nutrition composition analysis, discover that nutrition value of late-maturity type is higher than nutrition value ofprematurity type, coarse fibre and coarse protein and coarse fat of late-maturity type are 10.7%, 3.58%, 24.82% respectively, coarse fibre and coarse protein and coarse fat of prematurity type are 9.84%, 2.61%, 27.59% respectively, dry matter content of prematurity type is 92.63%, dry matter content of late-maturity type is 92.22%.

在枯黄期，第二、第三年生的早熟类猫尾草一年收割一次，枯草平均产量为10672.5 kg/hm~2，风干草平均产量为7575 kg/hm~2，选育出的群体平均高度为160.0cm，群体内植株株高较均一；在常规营养成分的分析中发现晚熟类的营养价值稍高于早熟类，晚熟类的粗蛋白是10.7％，粗脂肪是3.58％，粗纤维是24.82％，早熟类的粗蛋白是9.84％，粗脂肪是2.61％，粗纤维是27.59％，早熟类干物质含量92.63％，晚熟类干物质含量92.22％。

The protein which act on the phosphated tyrosine receptor kinase is phosphoinsitol 3 kinase , phospholipase C gamma and connective protein Shc, at last the signals can be transferred.

与酪氨酸磷酸化的Trk A相互作用的蛋白质是磷脂酶Cγ和磷脂酰肌醇(-3)激酶(PI3K)，及连接蛋白质Shc，最终传播NGF信号。

Erve growth factor can combine with its" receptor (one of tyrosine protein kinase receptor coded by proto - oncogene tyrosine kinase, TrkA), Causing the molecule of receptors to gather on the cell surface, and the tyrosine protein in cytoplasmic domain may be activated, three tyrosine base in the kinase domain of its" receptor and two tyrosine base at other domain be phosphated, thus, phosphated tyrosine receptor kinase became the bracket which absorb all kinds of connective proteins and kinases.

GF与其酪氨酸受体激酶受体(由原癌基因trk编码的一种酪氨酸蛋白激酶受体，TrkA)相结合，引起受体分子在细胞表面发生二聚体化，然后受体细胞内结构上酪氨酸活性被激活，使受体自身结构中位于激酶结构域的三个酪氨酸残基及在此结构域外的两个酪氨酸残基发生自身磷酸化，这样，磷酸化的酪氨酸受体激酶A便成为吸收各种连接蛋白质和酶的支架。

Focusing on non-phototropic hypocotyls 3 (NPH3), a protein known to be essential for phototropic responses, they examined its phosphorylation, the addition or removal of a phosphate group to the protein molecule.

小组主要分析了一种对于趋光性非常关键的蛋白NPH3，他们研究了这一蛋白的磷酸化作用。

Background:C-reactive protein is a phylogenetically highly conserved plasma protein,with homologs in vertebrates and many invertebrates,that participates in the systemic response to inflammation.

背景：C-反应蛋白是一种高度保守的血浆蛋白，在脊椎动物和多种非脊椎动物中存在同系物，参与全身炎症反应。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐