查询词典 protein-free

Methods Skp2 protein and p27 protein were examined by using immunohistochemical SP staining method in 48 cases of osteosarcoma and 16 cases of osteochondroma.

目的 探讨骨肉瘤中Skp2和p27蛋白的表达与患者临床病理和预后的关系以及两种蛋白之间的相关性。

Methods Skp2 protein and p27 protein were examined by using immunohistochemical SP staining method in 48 cases of osteosarcoma and 16 cases of osteochondroma.

用免疫组织化学SP法检测16例骨软骨瘤和48例骨肉瘤组织中Skp2和p27蛋白的表达情况。

Methods: Immunohistochemistry was used to detect the expressions of PTTG protein and c-myc protein in 37 osteosarcoma and 12 osteoenchondroma.

采用免疫组织化学的方法及图像分析技术检测37例骨肉瘤和12例骨软骨瘤中PTTG、c-myc蛋白的表达。并与骨肉瘤主要临床病理学参数及转移进行比较。

The recombinant protein was purified by Ni-NTA affinity chromatography and gel filtration chromatography. The inbred C57BL/6 strains and outbred KM strains of mice were subcutaneously immunized with the protein formulated by either adjuvant ISA720 or aluminium hydroxide.

表达产物通过Ni-NTA亲和层析和凝胶过滤层析方法纯化，再分别与ISA720和Al3佐剂乳化后，免疫C57BL/6和昆明小鼠，通过尾蚴膜反应和ELISA反应检测特异性抗体。

Methods:rThe protein from persons'ovarial carcinoma tissue and normal ovary was drawn,and the spots of protein were identified with the Swiss-Prot database.

取人卵巢正常及恶性肿瘤组织，提取蛋白质，进行双向电泳分离，银染获得其蛋白质电泳分离图谱对蛋白质斑点进行初步鉴定。

We generated transgenic mice that overexpress a heat shock protein 27 (Hsp27) and then to determine the effects of heat shock protein 27 on the isoproterenol-induced cardiac hypertrophy in early period.

通过转基因小鼠心肌特异性高表达人热休克蛋白27(Hsp27)模型，探讨人Hsp27对异丙肾上腺素(isoproteronol，ISO)诱导小鼠心脏是否存在早期抑制心肌肥厚的保护作用。

The aim of this study was to investigate the expression of mPGES-2 protein in mouse oviduct during the early pregnancy, pseudopregnancy, and estrous cycle by immunohistochemistry and Western blot. The regulation of mPGES-2 protein in mouse oviducts was also studied under delayed implantation, steroid hormonal treatments and oviductal ligation.

本实验主要利用早期妊娠、假孕、输卵管结扎、发情周期、激素调节以及延迟着床等实验模型，结合免疫组织化学和Western blot 的实验方法，详细研究了mPGES-2 蛋白在小鼠输卵管中的表达与调节。mPGES-2 蛋白主要定位于不同时期的伞部和壶腹部的上皮纤毛细胞中，在伞部和壶腹部肌层以及峡部和宫管连接处的上皮和肌层中未检测到mPGES-2 蛋白的表达。

Objective: to express OMP2b antigen protein of OMP36 from out membrane protein of Brucella ovis in XinJiang and study the probability for it as molecular bacterin and diagnose antigen.

以表达新疆绵羊种布鲁氏菌外膜抗原蛋白OMP36的表达蛋白OMP2b，探索其作为诊断抗原和分子疫苗的可能性。

The changes of muscle protein content and pH values were observed. Results Thymus and spleen coefficients in high and low dosage group were 9.1±2.3, 6.3±1.1, respectively, and those of in the controll were 6.5±1.6, 3.4±0.5.The contents of muscle protein (g/100 g) in high, medium, and low dose groups were 24.68±0.55, 22.48±0.54, 21.22±1.25, respectively, lower than that of the control (31.83±2.36, P.05). Paramorphia of lymphocyte in high dose ciprofloxacin treated group was observed.

结果 对照组胸腺和脾脏系数分别为(9.1±2.3)，(6.3±1.1)mg/g，高剂量组胸腺和脾脏系数分别为(6.5±1.6)，(3.4±0.5)mg/g，与对照组比较，差异均有统计学意义（P.05或P.01）；而中、低剂量组的脾脏指数与对照组比较，差异均有统计学意义(P.05)；环丙沙星各剂量组与对照组比较，肌肉pH值变化不明显；环丙沙星高、中、低剂量肌肉蛋白质含量分别为(24.68±0.55)，(22.48±0.54)，(21.22±1.25)g/100 g，与对照组(31.83±2.36)g/100 g比较明显下降(P.05)；与对照组比较，高剂量组脾淋巴细胞形态异常。

Methods Male New Zealand white rabbits were exposed to pentavalent arsenate and trivalent arsenite 5 mg/L for 18 weeks, respectively. Levels of thioredoxin, protein and non-protein sulfhydryl and the activity of thioredoxin reductase and glutathione reductase in liver, and the levels of inorganic arsenic, monomethylarsonic acid and dimethylarsinic acid in blood, urine, and hair were measured after that.

家兔以自由饮水方式慢性暴露于无机3价砷及5价砷，18周后，测定肝脏组织中蛋白巯基、非蛋白巯基、硫氧还蛋白水平，以及硫氧还蛋白还原酶、谷胱甘肽还原酶活性，同时检测血、尿及毛发中无机砷及其代谢产物甲基砷和二甲基砷。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐