查询词典 protein-free

The 20-HETE-induced increases in [Ca2+]i, whole-cell L-type Ca2+ channel currents and the amplitudes of Ca2+ transients were blocked by extracellular application of chelerythrine (an inhibitor of protein kinase C, PKC) and H-89 (an inhibitor of cAMP-dependent protein kinase, PKA).

上述20-HETE诱导的[Ca2+]i、全细胞L-型钙离子通道电流、钙瞬变幅度的增加等效应，可被蛋白激酶C抑制剂白屈菜红碱和cAMP依赖蛋白激酶抑制剂H-89阻滞。

Then the reaction rates of IgE and IgG in asthmatic patients serum to chenopodium album pollen were counted by immunoblotting. RESULTS: Twelve bands of protein were obtained through SDS PAGE. The molecular weight of each band was 92, 63.1, 61, 52, 45, 43, 39.7, 38.9, 34, 31.6, 28.4 and 18.5~12 ku. Immunobloting identified 4 protein bands recognized by sIgE, molecular weights being 92, 34, 31.6 and 18.5~12 ku, which reacted with 70%, 50%, 80% and 90% of asthmatic patient serum.

结果： 藜草花粉经电泳后得到12条蛋白质区带，分子质量依次为92， 63.1， 61， 52， 45， 43， 39.7， 38.9， 34， 31.6， 28.4和18.5~12 ku；免疫印迹可见4条sIgE反应带，蛋白分子质量依次为92， 34， 31.6和18.5~12 ku；与患者血清反应率分别是70%， 50%， 80%和90%；与血清sIgG结合的反应带有8条，蛋白分子质量为92， 61， 52， 45， 43， 39.7， 31.6和18.5~12 ku；与患者血清反应率分别是80%， 40%， 10%， 20%， 80%， 10%， 10%和100%。

The expression of the chimeric protein was shown by fluorescent microscope. The chimeric protein only existed in cytoplasm.

转染HEK293细胞后，荧光显微镜检测显示融合蛋白仅在细胞浆中表达。

Select chorioallantoic membrane vascular extract protein of different time points , Detect FGF2 and TGF-β2 protein in chorioallantoic membrane of this three-stage by Western blotting, judge whether to establish a model of dynamic vascular development successful.

选取不同时间点的尿囊膜血管，提取蛋白，用Western blotting技术检测FGF2和TGF-β2蛋白在血管发育的这三个阶段的表达量的变化，判断动态血管发育模型是否建立成功。

Methods The plasma levels of protein C activity and protein C antigen were measured using chromogenic assay and ELISA, respectively.

分别用发色底物法和ELISA测定血浆蛋白C活性和抗原。

OsCH2 protein in rice has 85%,57%,57%,60%, 58% and 61% homologous withzea, gentiana, coffea, lycopersicon, citrus and crocus respectively. OsHSP82 protein has 95%,94%,90%,90% and 89% homologous with wheat,barley, tobacco,tomato and arabidopsisrespectively.

水稻OsCH2蛋白与玉米、龙胆草、阿拉比卡咖啡豆、番茄、甜橙、番红花蛋白同源性分别为85％、57％、57％、60％、58％和61％，OsHSP82蛋白与小麦、大麦、烟草、番茄和拟南芥蛋白同源性分别为95％、94％、90％、90％和89％。

Recombinant adenovirus was identified by polymerase chain reColon cancer cell line SW480 was infected with recombinant adenovirus Ad-p27mt, and expression of p27 protein was detected by Western blot.of expressed p27 protein after Ad-p27mt transfected colon cancer cell.novirus framework plasmid pAdeasy-1 with pShuttle-CMV-hp27mt, 30％combinant adenovirus DNA contained the target gene.

Ad-p27mt的聚合酶链反应检测Ad-p27mt转染大肠癌细胞后的p27mt的表达。结果：①用含pShuttle-CMV-hp27mt转化含pAdeasy-1的超感受态BJ5183后，可获得约30％的阳性重组质粒。②聚合酶链反应检测表明重组腺病毒DNA中含有目的基因。重组腺病毒滴度为7.95×1015pfu/L。

Using the intracellular recording technique for monitoring the excitatory postsynaptic potentials of CA1 pyramidal neuron and delivering an activator of protein kinase C and/or inhibitors of PKC and 〓/calmodulin-dependent protein kinase Ⅱ into postsynaptic cell in hippocampal slices, we studied the role of postsynaptic PKC and CaMKⅡ in long-term potentiation induced by high frequency stimulation at the CA1 synapses made by Schaffer collateral/commissural and perforant pathway afferents.

用电生理学方法在大鼠海马脑片CA1区锥体细胞记录Schaffer侧枝和穿通通路到CA1神经元突触的兴奋性突触后电位，通过送蛋白激酶抑制剂和激活剂进入突触后细胞，我们研究了突触后蛋白激酶C和依赖钙/钙调蛋白的Ⅱ型蛋白激酶在高频刺激诱导的长时程增强产生和维持中的作用。

The determination of reducing sugar ,chitinase, soluble protein content of three cell inclusions to Hyphae within the cells and found that the different trend over time: the reducing sugar content of synergistic mixture so that in 25 h, the decline for the contrast of 18.91%; chitinase activity in between 5~20 h increased; soluble protein contently increased by the subsequent and decline in the short term.

测定菌丝细胞渗漏的还原糖、几丁质酶、可溶性蛋白等三种细胞内含物含量，发现其随时间变化趋势不同：增效组合使还原糖含量在25 h时，下降为对照的18.91%；几丁质酶活性在5~20 h之间增加；可溶性蛋白含量短期内上升随后下降。

GdCl3(10mg/kg) or the same volume of NS was continually injected of vein at 48h and 24h before LPS(5mg/kg) was injected in the male kunming species mouse. Then took out of liver or isolated KCs 30 minute after LPS was injected and assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of liver or KCs in vivo.Ⅱ. Isolated and cultured KCs of mouse, 1 h pretreatment with GdCl3; Culture medium with LPS(100ng/ml) was added and continuatively incubated 30 minute. Assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of KCs in vitro and detection effect of GdCl3 on its phagocytosis function in respectively.

Ⅰ 雄性昆明种小鼠在注射LPS（5mg/kg）前48h、24h，分别静脉注射GdCl3（10mg/kg ）或等量的生理盐水，于LPS或生理盐水注射后30min，分别取出肝脏或分离KCs，检测肝脏或KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平；Ⅱ体外培养小鼠KCs经GdCl3（100μM）预处理1h，加入含LPS（100ng/ml）的DMEM培养基继续孵育30min，分别检测体外培养KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平和GdCl3对其吞噬、分泌功能的影响。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。