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It is the first time to clone the BiVP gene from the bumblebee, Bombus ignitus and express it in Spodoptera frugiperda Sf-9 cell. The purified recombinant protein fulfilled the needs of further studying the bioactivity of this protein.

首次在红光熊蜂中克隆到蜂毒蛋白酶基因,在昆虫杆状病毒表达系统中进行了表达,并对表达产物进行了纯化,为深入研究BiVP的生物活性奠定了基础。

Aim: this article studied the separation condition of vitro gel of Brasenia schrebeVs tender leaves and its basic component, the separation rule of Zinc in various organs and biomacromolecule of Brasenia schrebeVs tender leaves, that laid the groundwork for deep-processing Brasenia schrebei; At the same time, the purification of protein rich in Zinc laid the groundwork for studying its structure, characteristics and the binding organism between Zinc and protein.

目的:研究莼菜嫩叶体外胶质的分离条件和其基本组成以及锌在莼菜各器官和嫩叶生物大分子中分布的规律,为莼菜深加工打下基础;分离纯化富锌蛋白,为研究其结构、性质以及锌与莼菜蛋白质的结合机制打下基础。

Canavalia ensiformis protein has relatively high nutritional value,but the unprocessed Canavalia ensiformis protein contains a number of anti nutritional factors such as hemagglutinin ,trypsin inhibitors that limit its used as human food and animal feed.

洋刀豆蛋白是一种具有较高营养价值的植物蛋白质,但是其中含有的一些抗营养因子,如血球凝集素(ConcanavalinA ,简称ConA)、胰蛋白酶抑制剂等,限制了它在人类食用植物蛋白和动物饲料中的应用,其中ConA是最主要的抗营养因子。

Optimum nutritional level of concentrated feeds of adult capreolus: optimum protein level of concentrated feeds of adult male capreolus in service period was 23.73%,concentrate of energy was 53.69g. To overwintering and normal development, adult male capreolus in service period needed digestible protein 53.69g, digestible energy 38.03MJ per day.

成年狍精饲料适宜营养水平:成年公狍配种期精饲料较适宜的蛋白质水平为23.73%、能量浓度为17.19,为保证公狍安全越冬及狍正常发育,成年公狍配种期每天需要供给可消化蛋白质53.69g,可消化能38.03MJ。

The result of protein concentration analysis, activity detection and sodium dodecyl sulphate - polyacrylamide gel electrophoresis demonstnated that protein content of IgG purified by ammonium sulfate is higher than that of caprylic acid - ammonium sulfate, but the purity and activity of IgG purified by caprylic acid - ammonium sulfate is far higher than that of ammonium sulfate.

本研究中对辛酸-硫酸铵法和硫酸铵法提取鸭血清中IgG进行了比较,经蛋白含量测定和SDS-PAGE电泳分析表明,虽然硫酸铵法提取蛋白含量要比辛酸-硫酸铵法高,但辛酸-硫酸铵法提取IgG的纯度和活性都明显高于硫酸铵法。

For the paramyxoviruses, the fusion protein catalyses this membrane merger and entry step, and it has been postulated that the F protein undergoes complex refolding during this process.

涉及这一过程的很多病毒蛋白是高度&专业&的。

Photosystem Ⅰ is a pigment-protein complex embedded in the photosynthetic membrane, which includes more than ten protein subunits, and catalyses the transfer of electrons from the PC to the Fd through a series of electron transfer components.

光系统Ⅰ是整合於光合膜上的由多个蛋白亚基组成的色素蛋白复合物,它在光合电子传递链中催化电子从PC经过一系列电子传递体到Fd的传递。

Twenty-three isolates of gray leaf spot pathogen, Cercospora zeae-maydis, collected from growing area of Northern China were clustered based on their soluble protein and isozyme profiles obtained through native PAGE in order to base physiologically the pathogen differentiation at protein or isozyme level.

利用聚丙烯酰胺凝胶电泳技术,对采自北方玉米主产区的23个玉米灰斑病菌菌株进行可溶性蛋白质和同工酶电泳图谱分析及聚类分析,从蛋白质和酶学的多态性水平上分析玉米灰斑病菌的生理分化特征。

METHODS: NE has been shown to be a potent stimulator for neonatal rat ventricular myocyte hypertrophy in vitro. We used the activator of PPAR γ, the inhibitor of protein kinase C chelerythrine to influence protein content.3H?

在培养新生大鼠心肌细胞中,采用RSG,PKC的激动剂佛波醇酯,NE和PKC的阻断剂白屈菜季氨碱观察罗格列酮在NE和PMA诱导心肌肥大中对PKC活性和c?

However it was shown its eventually inhibiting effect on apoptotic myocyte cells during I〓 R〓h (P<0.05); The PKC inhibitors chelerythrine and scutellarein have no effect on expression of Fas protein, whereas they can significantly upregulate the expression of Bcl-2 protein.

4PKC激活剂PMA可下调Fas基因的蛋白表达,对Bcl-2蛋白的表达也有抑制作用,它对缺血30min、再灌注24h后心肌凋亡细胞的最终影响为抑制效应(P<0.05),而PKC抑制剂CHE、灯盏花素对Fas基因蛋白表达无明显干预影响,但对Bcl-2蛋白表达呈显著上调作用,故对心肌细胞凋亡的抑制作用更为明显(P<0.01)。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在,'歼灭战perfuming自己与胡努特,那麼,他来到和SAT 。

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