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To predict the effect of site-specific mutation on protein stability and function has been an important issue of protein science.

预测特定位置突变所引起的蛋白质稳定性改变是生物学上的重要议题,将序列及结构资讯有效的转换为能量参数,将有助於蛋白质稳定性及功能的分析。

Methods The β-sitosterol and/or total protein of Pinella were incubated at different concentrations with cervical cancer cell line SiHa. The effects of β-sitosterol and/or total protein of Pinella on the viability of cells were tested by MTT assay.

在子宫颈癌细胞SiHa的培养基中加入不同浓度的β-谷甾醇和/或掌叶半夏总蛋白培养1~7d,采用MTT比色法检测细胞的活性。

Results When sodium arsenite treatment and cigarette smoke solution treatment were used together or separately, protein expression of Bcl-2 decreased; Protein expression of Bax and the number of apoptotic cells increased.

结果 亚砷酸钠和香烟烟气溶液单独作用或者联合作用都能够使细胞Bcl-2蛋白表达减少、Bax蛋白表达增加、凋亡细胞数增加。

Results When sodium arsenite treatment and cigarette smoke solution treatment were used together or separately,protein expression of Bcl2 decreased;Protein expression of Bax and the number of apoptotic cells increased.

利用免疫印迹法检测细胞的Bcl2和Bax表达,利用磷脂酰丝氨酸外翻分析法检测各组细胞凋亡,同时采用2×2析因设计的方差分析研究两者的交互作用。

Soya is a high quality protein on its own which can be regarded as equal to meat protein.

大豆是一种优质蛋白本身可以被视为相当於肉类蛋白质。

By partitioning the protein space in spherical polar coordinates, the space density of the main elements in protein molecules could be obtained easily and quickly.

实验结果表明,该方法可作为各种结构蛋白质相似性比较的辅助手段,不仅能较好地反映蛋白质分子的空间结构特征,而且还有令人满意的时间复杂性,对大分子的相似性比较及进一步分类将有重要意义。

The result indicates that apple seed is rich in protein arid fat, the content of crude protein in fuji and new red star apple seed is 48.85% and 49.55%, respectively, and the content of crude fat is 23.69% and 24.32%, respectively. In addition, apple seed oil is rich in unsaturated fatty acids, especially oleic acid and linoleic acid.

结果表明,富士和新红星苹果籽中富含粗蛋白和粗脂肪,粗蛋白含量分别为48.85%和49.55%,粗脂肪含量分别为23.69%和24.32%;苹果籽油中富含不饱和脂肪酸,尤其是油酸和亚油酸,两者在富士苹果籽油中含量分别为37.49%和51.40%,在新红星苹果籽油中含量分别为38.55%和50.70%。

Rice stem borer resistive to Bt is not selected out by rearing with high concentration Bt protein in short time and low concentration Bt protein in long time.

采用高浓度Bt制剂短时饲喂和低浓度Bt制剂整代饲喂两种途径对二化螟抗Bt品系进行了初步的选育,饲养的三代二化螟均没有对Bt制剂产生抗性。

When EGTA (Ca〓 chelator), Verapmil and LaCl〓(Ca〓 channel blockers) were used to treat tomato fruit at green mature and strawberry fruit at white stage with ethylene, they could reverse ethylene-induced increase in ethylene production in tomato and strawberry, PG activity, lycopene content, soluble protein content in cell wall in tomato and degradation in soluble protein in strawberry, which indicated blocking Ca〓 channel in plasma membrane or chelating Ca〓 can decrease intracellular Ca〓, further inhibite ethylene-induced maturation, ripening and senescence of fruit.

用质膜钙通道阻断剂异博定、钙通道Ca〓竞争性抑制剂以及Ca〓专一性螯合剂与乙烯一起处理绿熟期番茄果实和乳白期草莓果实,均可抑制乙烯诱导番茄和草莓的乙烯生成、番茄PG活性的提高、番茄红素含量的增加和细胞壁可溶性蛋白含量的增加以及草莓可溶性蛋白的分解,表明阻断质膜Ca〓通道或螯合胞外Ca〓以减少胞内Ca〓能抑制乙烯对果实的催熟作用,间接证明乙烯对果实成熟衰老的诱导与胞外Ca〓内流引起的胞质Ca〓浓度的增加密切相关,表明乙烯信号转导可能与钙信使有关,调节胞质Ca〓浓度可调节乙烯对果实的催熟作用。

We established baculovirus expression vectors of 49ku ES structural protein gene and obtained recombinant protein.

本实验将本地毛形线虫ES抗原的49ku结构基因构建到杆状病毒表达载体上,并在昆虫细胞中得到表达。

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Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。