查询词典 protein-free

Namely, we assume that there is the repulsive force between amino acid residue pairs in the protein molecule, which this force prevents the any two residues, which occur far apart in the amino acid sequence of the protein, come together in a contact.

即，假设蛋白质内的氨基酸残基之间存在着一种排斥力，它阻碍着蛋白质氨基酸序列中相隔较远的任意两个残基彼此靠拢到一个接触点。

The following conclusions have been reached, milk has an obvious absorption in the mid-infrared band, and the locations of the absorption peak are consistent with the attributes of molecule functional group analysis and the information from FOSS Company; the effects of the factors on the repeatability are below 3‰; For the quantitative analysis, the method of factorial design is applied to optimize the calibration set. The outliers are determined considering the residual variance distribution. The correlations are 0.990 and 0.986 for fat and protein, respectively. The Root Mean of Square Error of Prediction is 0.121 and 0.111 for fat and protein, respectively. With the application of the methods of wavelengths selection, the numbers of wavelength is decreased greatly from 3301 to 100. The speed is accelerated and the accuracy of the calibration is improved to some extent.

结果表明：牛奶在中红外的吸收特性明显，吸收峰的位置与分子结构的谱带归属以及乳品分析仪器的成功厂商FOSS公司提供的资料基本一致；各种因素对光谱测量影响的CV值基本可以控制在3‰以下；在定量检测中，通过析因设计方法优选校正集样品，结合样品的残差图确定奇异点，脂肪模型和蛋白模型的相关系数分别为0.990和0.986，预测均方根误差分别为0.121和0.111，而在采用波长选择对模型进行优化后，波长从3301个减为100个以内，加快了运算的速度，同时还在一定程度上提高了模型的精度。

The cloning, sequencing, homological blasting and Northern blotting results of 5 desiccation-induced cDNAs and 3 phosphate induced cDNAs implied that signal transduction induced by desiccation, regulatory gene cascades and functional genes such as G protein, protein kinase, VP3and MAD3-like genes might be involved in dehydration in the resurrection plant Boea hygrometrica.

对其中5个脱水特异诱导表达的cDNA（包括可能与复苏能力有关的cDNA）和3个磷酸盐处理诱导表达的cDNA进行克隆测序、同源性探测和Northern杂交检测表明，牛耳草脱水过程中诱导表达的基因可能涉及到脱水胁迫的信号转导、调节基因的级联作用（VP3，MAD3样基因等）、结构基因产物调节细胞结构在脱水胁迫中的稳定性等。

1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异；采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异；采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

A protein of the retina, especially the protein constituent of rhodopsin,that makes up one of the visual pigments.

视蛋白视蛋白，尤指由视黄醛蛋白组成的元素，它是组成视色素的元素之一

Inherited mutations in rhodopsin, the light sensing protein of rods cells, are the single most common cause of one form of RP and the majority of these mutations cause protein misfolding.

遗传突变的视紫红质，光传感蛋白的燃料棒细胞，单是最常见的一种形式的反相和大多数这些突变导致蛋白质错误折叠。

Ribosome affect the protein synthesis, we could understand the situation of protein synthesis using this method.

核糖体核蛋白影响着蛋白质的合成，通过对小鼠肝细胞核糖体蛋白含量的测定可以了解核糖体参与蛋白合成的情况。

Objective: To study the expression and purification of a fusion protein of ricin A chain and green fluorescent protein.

目的：表达蓖麻毒素A链与绿色荧光蛋白的融合蛋白，用於蓖麻毒素A链在细胞内的转运机理研究。

Ricin is one kind of toxic protein that can inhibit the protein synthesis.

蓖麻毒素是一种毒蛋白，能抑制蛋白质合成。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。