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The protein, known as bone morphogenetic protein, or BMP, is used to spur bone growth.

这种被称为骨形成蛋白的人造蛋白质被用于刺激骨骼生长。

OBJECTIVE: To explore the feasibility to construct human bone morphogenetic protein-2 eukaryotic expression vector labeled with green fluorescent protein.

目的:验证构建绿色荧光蛋白标记的人骨诱导形成蛋白2真核表达载体的可行性。

Results The recombinant MPT 64 protein existed in inclusion bodies of E.coli.and amounted to 20%- 30% of totle cell protein.

具有天然结构和高纯度的重组融合蛋白有可能成为有效的结核病血清学诊断的候选抗原。

Protein multiple sequence alignment is an important bioinformatics tools. It has important applications in biological evolution analysis and protein structure prediction.

蛋白质多序列比对是一种重要的生物信息学工具,在生物的进化分析以及蛋白质的结构预测方面有着重要的应用。

Hexon protein gene of egg drop syndrome virus, named strain E05 which was isolated from breeding muscovy duck with irregular egg dropping was amplified by PCR and cloned into pMD18-T vector. The recombinant plasmids were identified by digested restriction endonuclease and PCR. The result showed that the nucleotide length of hexon protein gene encoding 910 amino acids was 2733 bp.

运用PCR技术从表现产蛋异常的种番鸭分离鉴定的减蛋综合征病毒 E05株中分段扩增六邻体蛋白基因,分别与克隆载体pMD18-T连接后,转化感受态细胞,对经PCR鉴定和酶切鉴定均为阳性的克隆进行测序,结果表明,E05株六邻体蛋白基因长度为2733 bp,共编码910个氨基酸。

The recombinant baculovirus was transfected into sf-9 cells by CellFectin reagent. SDS-PAGE and Western-blot analysis showed that the expressed protein was 37ku in molecular mass and exhibited specific immunological activity. These results provide foundation for development of a subunit vaccine with the expressed σC protein against Muscovy duck reovirus infection.

在脂质体介导下将rBacmid-σC转染sf-9昆虫细胞,获得重组杆状病毒rBV-σC;SDS-PAGE和Western-blot分析表明,在sf-9细胞中表达了分子质量约37ku的σC蛋白,该蛋白能与原核表达的σC蛋白免疫小鼠血清发生特异性免疫反应,这为以表达的σC蛋白为抗原的亚单位疫苗的研制奠定了基础。

Heme iron, one of two types of iron found in foods, is bound to either the blood protein hemoglobin or the muscle protein myoglobin.

食物中的两种铁的类型之一的血红素铁与血红蛋白和肌红蛋白均有关。

The N protein concentration in the supernatants of virus-infected cells were reduced at different levels in all ER and cytoplasma localized intracellular antibody expressing cell lines, however, the intracellular N protein synthesis in above cell lines was not significantly affected.

利用病毒微量滴定免疫荧光方法,对细胞内抗体与病毒的增殖关系进行了研究,证实无论在内质网还是在胞质,抗核蛋白细胞内抗体都能够抑制病毒的增殖,具有抗病毒活性。

The optimal diagnostic model was developed by support vector machine algorithm with two protein peaks at Mass/Charge 3939 and 5909 respectively. The expression of protein peak at m/z 3939 was higher in chronic eczema and neurodermatitis than in MF, while that at m/z 5909 appeared to express in an opposite way.

结果 从15例蕈样肉芽肿与15例慢性湿疹、神经性皮炎患者的血清蛋白质谱中共检测出329个蛋白峰,从中筛选出30个表达差异有统计学意义(P 0.01)的蛋白标志物。

Nighty rats were randomly divided into the groups of control, myocardial infarction and doxycycline, which were given orally 48 hours before and 48 hours after MI with 30mg/Kg per day. Protein and mRNA extraction was done on left ventricular samples containing scar and myocardium together. Samples were assayed from 1 day to 4 weeks post-Mi. The activity of MMPs was measured by zymography and collagen amount by the method of chloramine T, the ratio of II III collagen was assessed by immunohistochemical stain. Protein and mRNA of MMP-2, 9, TIMP-1 were determined by Western-Blot and RT-PCR.

分为对照组、心梗组和治疗组(D组,术前两天至术后两天,口服Doxycycline,30mg/kg/天),分别于术后第一天、术后一周、术后两周、术后四周取心肌组织,采用免疫组化测定胶原含量和Ⅰ/Ⅲ胶原比例,酶谱法测定心梗后MMP-2,9活性蛋白的表达规律,Western Blotting进一步确定酶谱法中所消化条带蛋白的属性,逆转录-聚合链反应法测定心梗后MMP-2,9和TIMP-1的mRNA的变化规律,运用Acuson Sequoia 512超声心动图机分别测定心梗组、治疗组在两周和四周时左室前壁、后壁厚度以及左心室舒张末内径和左室射血分数。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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